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High Electrochemiluminescence from Ru(bpy)(3) (2+) Embedded Metal–Organic Frameworks to Visualize Single Molecule Movement at the Cellular Membrane

Direct imaging of single‐molecule and its movement is of fundamental importance in biology, but challenging. Herein, aided by the nanoconfinement effect and resultant high reaction activity within metal–organic frameworks (MOFs), the designed Ru(bpy)(3) (2+) embedded MOF complex (RuMOFs) exhibits br...

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Detalles Bibliográficos
Autores principales: Li, Binxiao, Huang, Xuedong, Lu, Yanwei, Fan, Zihui, Li, Bin, Jiang, Dechen, Sojic, Neso, Liu, Baohong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9762315/
https://www.ncbi.nlm.nih.gov/pubmed/36328787
http://dx.doi.org/10.1002/advs.202204715
Descripción
Sumario:Direct imaging of single‐molecule and its movement is of fundamental importance in biology, but challenging. Herein, aided by the nanoconfinement effect and resultant high reaction activity within metal–organic frameworks (MOFs), the designed Ru(bpy)(3) (2+) embedded MOF complex (RuMOFs) exhibits bright electrochemiluminescence (ECL) emission permitting high‐quality imaging of ECL events at single molecule level. By labeling individual proteins of living cells with single RuMOFs, the distribution of membrane tyrosine‐protein‐kinase‐like7 (PTK7) proteins at low‐expressing cells is imaged via ECL. More importantly, the efficient capture of ECL photons generated inside the MOFs results in a stable ECL emission up to 1 h, allowing the in operando visualization of protein movements at the cellular membrane. As compared with the fluorescence observation, near‐zero ECL background surrounding the target protein with the ECL emitter gives a better contrast for the dynamic imaging of discrete protein movement. This achievement of single molecule ECL dynamic imaging using RuMOFs will provide a more effective nanoemitter to observe the distribution and motion of individual proteins at living cells.