A modified hydrogel production protocol to decrease cellular content

PURPOSE: To analyze the cytotoxicity and cell in porcine-derived decellularized skin matrix. METHODS: We analyzed the effect of multiple decellularization processes by histological analysis, DNA quantification, and flow cytometry. Subsequently, we analyzed the most appropriate hydrogel concentration...

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Detalles Bibliográficos
Autores principales: Braga, Gabriela Catão Diniz, Camargo, Cristina Pires, Harmsen, Martin Conrad, Correia, Aristides Tadeu, Souza, Sonia, Seelaender, Marilia, Nunes, Viviane Araujo, dos Santos, Jeniffer Farias, Neri, Elida Adalgisa, Valadão, Iuri Cordeiro, Moreira, Luiz Felipe Pinho, Gemperli, Rolf
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia 2022
Materias:
Original Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9762429/
https://www.ncbi.nlm.nih.gov/pubmed/36542042
http://dx.doi.org/10.1590/acb371005
Descripción
Sumario:PURPOSE: To analyze the cytotoxicity and cell in porcine-derived decellularized skin matrix. METHODS: We analyzed the effect of multiple decellularization processes by histological analysis, DNA quantification, and flow cytometry. Subsequently, we analyzed the most appropriate hydrogel concentration to minimize cytotoxicity on fibroblast culture and to maximize cell proliferation. RESULTS: After the fourth decellularization, the DNA quantification showed the lowest DNA concentration (< 50 ng/mg). Histological analysis showed no cell components in the hydrogel. Moreover, hematoxylin and eosin showed a heterogeneous structure of collagen fibers. The best hydrogel concentration ranged from 3 to 25%, and there was no significant difference between the 24 hours and seven days. CONCLUSIONS: The process of hydrogel production was effective for removing cells and DNA elements. The best hydrogel concentration ranged from 3 to 25%.

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