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The Cytostatic Action of Dinitrosyl Iron Complexes with Glutathione on Escherichia coli Cells Is Mediated by Nitrosonium Cations Released from These Complexes
This study demonstrates a bacteriostatic effect of binuclear dinitrosyl iron complexes with glutathione on Escherichia coli TN300 cells. It has been quantified by the colony formation assay. The bacteriostatic effect exerted by these complexes increases considerably in the presence of diethyldithioc...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Pleiades Publishing
2022
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9762666/ https://www.ncbi.nlm.nih.gov/pubmed/36567970 http://dx.doi.org/10.1134/S0006350922050207 |
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author | Vanin, A. F. Telegina, V. I. Mikoyan, V. D. Tkachev, N. A. Vasilieva, S. V. |
author_facet | Vanin, A. F. Telegina, V. I. Mikoyan, V. D. Tkachev, N. A. Vasilieva, S. V. |
author_sort | Vanin, A. F. |
collection | PubMed |
description | This study demonstrates a bacteriostatic effect of binuclear dinitrosyl iron complexes with glutathione on Escherichia coli TN300 cells. It has been quantified by the colony formation assay. The bacteriostatic effect exerted by these complexes increases considerably in the presence of diethyldithiocarbamate. Our results suggest that this effect is caused by the intense release of nitrosonium cations, NO(+), from the complexes, which decompose under the action of diethyldithiocarbamate. A similar effect is observed when E. coli cells are treated with diethyldithiocarbamate 40 min after the addition of sodium nitrite or S-nitrosoglutathione. Notably, the level of dinitrosyl iron complexes observed in the bacterial cells due to the effects of sodium nitrite or S-nitrosoglutathione is almost the same as that obtained after treatment with glutathione-containing complexes. The bacteriostatic effects of the NO molecules released from nitrite or S-nitrosoglutathione during their brief interaction with bacteria were significantly smaller than the bacteriostatic effect of NO(+). We deduce therefrom that the nitrosonium cations released from DNICs are responsible for the observed bacteriostatic effect of these complexes in E. coli cells. |
format | Online Article Text |
id | pubmed-9762666 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Pleiades Publishing |
record_format | MEDLINE/PubMed |
spelling | pubmed-97626662022-12-20 The Cytostatic Action of Dinitrosyl Iron Complexes with Glutathione on Escherichia coli Cells Is Mediated by Nitrosonium Cations Released from These Complexes Vanin, A. F. Telegina, V. I. Mikoyan, V. D. Tkachev, N. A. Vasilieva, S. V. Biophysics (Oxf) Cell Biophysics This study demonstrates a bacteriostatic effect of binuclear dinitrosyl iron complexes with glutathione on Escherichia coli TN300 cells. It has been quantified by the colony formation assay. The bacteriostatic effect exerted by these complexes increases considerably in the presence of diethyldithiocarbamate. Our results suggest that this effect is caused by the intense release of nitrosonium cations, NO(+), from the complexes, which decompose under the action of diethyldithiocarbamate. A similar effect is observed when E. coli cells are treated with diethyldithiocarbamate 40 min after the addition of sodium nitrite or S-nitrosoglutathione. Notably, the level of dinitrosyl iron complexes observed in the bacterial cells due to the effects of sodium nitrite or S-nitrosoglutathione is almost the same as that obtained after treatment with glutathione-containing complexes. The bacteriostatic effects of the NO molecules released from nitrite or S-nitrosoglutathione during their brief interaction with bacteria were significantly smaller than the bacteriostatic effect of NO(+). We deduce therefrom that the nitrosonium cations released from DNICs are responsible for the observed bacteriostatic effect of these complexes in E. coli cells. Pleiades Publishing 2022-12-19 2022 /pmc/articles/PMC9762666/ /pubmed/36567970 http://dx.doi.org/10.1134/S0006350922050207 Text en © Pleiades Publishing, Inc. 2022, ISSN 0006-3509, Biophysics, 2022, Vol. 67, No. 5, pp. 761–767. © Pleiades Publishing, Inc., 2022.Russian Text © The Author(s), 2022, published in Biofizika, 2022, Vol. 67, No. 5, pp. 942–950. This article is made available via the PMC Open Access Subset for unrestricted research re-use and secondary analysis in any form or by any means with acknowledgement of the original source. These permissions are granted for the duration of the World Health Organization (WHO) declaration of COVID-19 as a global pandemic. |
spellingShingle | Cell Biophysics Vanin, A. F. Telegina, V. I. Mikoyan, V. D. Tkachev, N. A. Vasilieva, S. V. The Cytostatic Action of Dinitrosyl Iron Complexes with Glutathione on Escherichia coli Cells Is Mediated by Nitrosonium Cations Released from These Complexes |
title | The Cytostatic Action of Dinitrosyl Iron Complexes with Glutathione on Escherichia coli Cells Is Mediated by Nitrosonium Cations Released from These Complexes |
title_full | The Cytostatic Action of Dinitrosyl Iron Complexes with Glutathione on Escherichia coli Cells Is Mediated by Nitrosonium Cations Released from These Complexes |
title_fullStr | The Cytostatic Action of Dinitrosyl Iron Complexes with Glutathione on Escherichia coli Cells Is Mediated by Nitrosonium Cations Released from These Complexes |
title_full_unstemmed | The Cytostatic Action of Dinitrosyl Iron Complexes with Glutathione on Escherichia coli Cells Is Mediated by Nitrosonium Cations Released from These Complexes |
title_short | The Cytostatic Action of Dinitrosyl Iron Complexes with Glutathione on Escherichia coli Cells Is Mediated by Nitrosonium Cations Released from These Complexes |
title_sort | cytostatic action of dinitrosyl iron complexes with glutathione on escherichia coli cells is mediated by nitrosonium cations released from these complexes |
topic | Cell Biophysics |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9762666/ https://www.ncbi.nlm.nih.gov/pubmed/36567970 http://dx.doi.org/10.1134/S0006350922050207 |
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