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Occurrence and Diversity of Listeria monocytogenes Isolated from Two Pig Manure Treatment Plants in France

The presence of Listeria monocytogenes in piggery effluents intended for irrigation crops may be a source of bacterial dissemination in agriculture. The occurrence and diversity of L. monocytogenes in the farm environment were examined in two pig manure treatment systems (S1 and S2). Samples collect...

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Autores principales: Denis, Martine, Ziebal, Christine, Boscher, Evelyne, Picard, Sylvie, Perrot, Morgane, Nova, Meryl Vila, Roussel, Sophie, Diara, Arnaud, Pourcher, Anne-Marie
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Japanese Society of Microbial Ecology / Japanese Society of Soil Microbiology / Taiwan Society of Microbial Ecology / Japanese Society of Plant Microbe Interactions / Japanese Society for Extremophiles 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9763045/
https://www.ncbi.nlm.nih.gov/pubmed/36372433
http://dx.doi.org/10.1264/jsme2.ME22019
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author Denis, Martine
Ziebal, Christine
Boscher, Evelyne
Picard, Sylvie
Perrot, Morgane
Nova, Meryl Vila
Roussel, Sophie
Diara, Arnaud
Pourcher, Anne-Marie
author_facet Denis, Martine
Ziebal, Christine
Boscher, Evelyne
Picard, Sylvie
Perrot, Morgane
Nova, Meryl Vila
Roussel, Sophie
Diara, Arnaud
Pourcher, Anne-Marie
author_sort Denis, Martine
collection PubMed
description The presence of Listeria monocytogenes in piggery effluents intended for irrigation crops may be a source of bacterial dissemination in agriculture. The occurrence and diversity of L. monocytogenes in the farm environment were examined in two pig manure treatment systems (S1 and S2). Samples collected over the course of one year consisted of manure, the liquid fraction of treated manure (lagoon effluent), and soil surrounding the lagoon. L. monocytogenes was enumerated using the Most Probable Number (MPN) method, serotyped by PCR, genotyped by pulsed-field gel electrophoresis (PFGE), and sequenced for multilocus sequence typing (MLST). L. monocytogenes was detected in 92% of manure samples and in approximately 50% of lagoon effluent and soil samples. Concentrations ranged between 5 and 10(3) MPN 100‍ ‍mL(–1). Serogroups IIa, IIb, and IVb were identified. Diversity was high with 44 PFGE profiles (252 isolates) and 17 clonal complexes (CCs) (96 isolates) with higher diversity in manure at site S1 supplied by four farms. Some PFGE profiles and CCs identified in manure or in pig feces from a previous study were also detected in lagoons and/or soil, reflecting pig L. monocytogenes circulation throughout the manure treatment and in the vicinity of the sampling sites. However, some PFGE profiles and CCs were only found in the lagoon and/or in soil, suggesting an origin other than pigs. The present study highlights the limited ability of biological treatments to eliminate L. monocytogenes from pig manure. The persistence of some PFGE profiles and CCs throughout the year in the lagoon and soil shows the ability of L. monocytogenes to survive in this type of environment.
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spelling pubmed-97630452023-01-03 Occurrence and Diversity of Listeria monocytogenes Isolated from Two Pig Manure Treatment Plants in France Denis, Martine Ziebal, Christine Boscher, Evelyne Picard, Sylvie Perrot, Morgane Nova, Meryl Vila Roussel, Sophie Diara, Arnaud Pourcher, Anne-Marie Microbes Environ Regular Paper The presence of Listeria monocytogenes in piggery effluents intended for irrigation crops may be a source of bacterial dissemination in agriculture. The occurrence and diversity of L. monocytogenes in the farm environment were examined in two pig manure treatment systems (S1 and S2). Samples collected over the course of one year consisted of manure, the liquid fraction of treated manure (lagoon effluent), and soil surrounding the lagoon. L. monocytogenes was enumerated using the Most Probable Number (MPN) method, serotyped by PCR, genotyped by pulsed-field gel electrophoresis (PFGE), and sequenced for multilocus sequence typing (MLST). L. monocytogenes was detected in 92% of manure samples and in approximately 50% of lagoon effluent and soil samples. Concentrations ranged between 5 and 10(3) MPN 100‍ ‍mL(–1). Serogroups IIa, IIb, and IVb were identified. Diversity was high with 44 PFGE profiles (252 isolates) and 17 clonal complexes (CCs) (96 isolates) with higher diversity in manure at site S1 supplied by four farms. Some PFGE profiles and CCs identified in manure or in pig feces from a previous study were also detected in lagoons and/or soil, reflecting pig L. monocytogenes circulation throughout the manure treatment and in the vicinity of the sampling sites. However, some PFGE profiles and CCs were only found in the lagoon and/or in soil, suggesting an origin other than pigs. The present study highlights the limited ability of biological treatments to eliminate L. monocytogenes from pig manure. The persistence of some PFGE profiles and CCs throughout the year in the lagoon and soil shows the ability of L. monocytogenes to survive in this type of environment. Japanese Society of Microbial Ecology / Japanese Society of Soil Microbiology / Taiwan Society of Microbial Ecology / Japanese Society of Plant Microbe Interactions / Japanese Society for Extremophiles 2022 2022-11-12 /pmc/articles/PMC9763045/ /pubmed/36372433 http://dx.doi.org/10.1264/jsme2.ME22019 Text en 2022 by Japanese Society of Microbial Ecology / Japanese Society of Soil Microbiology / Taiwan Society of Microbial Ecology / Japanese Society of Plant Microbe Interactions / Japanese Society for Extremophiles. https://creativecommons.org/licenses/by/3.0/This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Regular Paper
Denis, Martine
Ziebal, Christine
Boscher, Evelyne
Picard, Sylvie
Perrot, Morgane
Nova, Meryl Vila
Roussel, Sophie
Diara, Arnaud
Pourcher, Anne-Marie
Occurrence and Diversity of Listeria monocytogenes Isolated from Two Pig Manure Treatment Plants in France
title Occurrence and Diversity of Listeria monocytogenes Isolated from Two Pig Manure Treatment Plants in France
title_full Occurrence and Diversity of Listeria monocytogenes Isolated from Two Pig Manure Treatment Plants in France
title_fullStr Occurrence and Diversity of Listeria monocytogenes Isolated from Two Pig Manure Treatment Plants in France
title_full_unstemmed Occurrence and Diversity of Listeria monocytogenes Isolated from Two Pig Manure Treatment Plants in France
title_short Occurrence and Diversity of Listeria monocytogenes Isolated from Two Pig Manure Treatment Plants in France
title_sort occurrence and diversity of listeria monocytogenes isolated from two pig manure treatment plants in france
topic Regular Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9763045/
https://www.ncbi.nlm.nih.gov/pubmed/36372433
http://dx.doi.org/10.1264/jsme2.ME22019
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