Targeted integration in CHO cells using CRIS-PITCh/Bxb1 recombinase–mediated cassette exchange hybrid system

ABSTRACT: Recombinant Chinese hamster ovary (CHO) cell line development for complex biotherapeutic production is conventionally based on the random integration (RI) approach. Due to the lack of control over the integration site and copy number, RI-generated cell pools are always coupled with rigorou...

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Detalles Bibliográficos
Autores principales: Ghanbari, Samaneh, Bayat, Elham, Azizi, Masoumeh, Fard-Esfahani, Pezhman, Modarressi, Mohammad Hossein, Davami, Fatemeh
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Berlin Heidelberg 2022
Materias:
Applied Genetics and Molecular Biotechnology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9763083/
https://www.ncbi.nlm.nih.gov/pubmed/36536089
http://dx.doi.org/10.1007/s00253-022-12322-1

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