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Tear-derived exosomal biomarkers of Graves’ ophthalmopathy
Graves’ ophthalmopathy (GO), the most frequent extrathyroidal manifestation of Graves’ disease (GD), can lead to a significant decline in the quality of life in patients. Exosomes, which contain proteins, lipids and DNA, play important roles in the pathological processes of various diseases. However...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Frontiers Media S.A.
2022
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9763563/ https://www.ncbi.nlm.nih.gov/pubmed/36561758 http://dx.doi.org/10.3389/fimmu.2022.1088606 |
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author | Shi, Ting-Ting Zhao, Ru-Xuan Xin, Zhong Hou, Zhi-Jia Wang, Hua Xie, Rong-Rong Li, Dong-Mei Yang, Jin-Kui |
author_facet | Shi, Ting-Ting Zhao, Ru-Xuan Xin, Zhong Hou, Zhi-Jia Wang, Hua Xie, Rong-Rong Li, Dong-Mei Yang, Jin-Kui |
author_sort | Shi, Ting-Ting |
collection | PubMed |
description | Graves’ ophthalmopathy (GO), the most frequent extrathyroidal manifestation of Graves’ disease (GD), can lead to a significant decline in the quality of life in patients. Exosomes, which contain proteins, lipids and DNA, play important roles in the pathological processes of various diseases. However, their roles in Graves’ ophthalmopathy are still unclear. We aimed to isolate exosomes and analyze the different exosomal proteins. Tear fluids were collected from twenty-four GO patients, twenty-four GD patients and sixteen control subjects. The numbers of tear exosomes were assayed using nanoparticle tracking analysis. A Luminex 200 kit and ELISA kit were used to confirm the different cytokine concentrations in serum. Extraocular muscle from GO patients and controls was extracted, and western blotting was used to assay the levels of Caspase-3 and complement C4A. Our study demonstrated that the number of tear exosomes differ from GD patients and control. The expression levels of cytokines, including IL-1 and IL-18, were significantly increased in the tear exosomes and serum from GO patients compared with GD patients and controls. The levels of the exosomal proteins Caspase-3, complement C4A and APOA-IV were significantly increased in GO patients compared to GD patients and controls. Orbital fibroblasts from GO patients showed significantly higher levels of Caspase-3 and complement C4A than those from controls. The levels of serum APOA-IV in GO patients were significantly higher than those in GD patients and controls. Specific proteins showed elevated expression in tear exosomes from GO patients, indicating that they may play important roles in GO pathogenesis. |
format | Online Article Text |
id | pubmed-9763563 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-97635632022-12-21 Tear-derived exosomal biomarkers of Graves’ ophthalmopathy Shi, Ting-Ting Zhao, Ru-Xuan Xin, Zhong Hou, Zhi-Jia Wang, Hua Xie, Rong-Rong Li, Dong-Mei Yang, Jin-Kui Front Immunol Immunology Graves’ ophthalmopathy (GO), the most frequent extrathyroidal manifestation of Graves’ disease (GD), can lead to a significant decline in the quality of life in patients. Exosomes, which contain proteins, lipids and DNA, play important roles in the pathological processes of various diseases. However, their roles in Graves’ ophthalmopathy are still unclear. We aimed to isolate exosomes and analyze the different exosomal proteins. Tear fluids were collected from twenty-four GO patients, twenty-four GD patients and sixteen control subjects. The numbers of tear exosomes were assayed using nanoparticle tracking analysis. A Luminex 200 kit and ELISA kit were used to confirm the different cytokine concentrations in serum. Extraocular muscle from GO patients and controls was extracted, and western blotting was used to assay the levels of Caspase-3 and complement C4A. Our study demonstrated that the number of tear exosomes differ from GD patients and control. The expression levels of cytokines, including IL-1 and IL-18, were significantly increased in the tear exosomes and serum from GO patients compared with GD patients and controls. The levels of the exosomal proteins Caspase-3, complement C4A and APOA-IV were significantly increased in GO patients compared to GD patients and controls. Orbital fibroblasts from GO patients showed significantly higher levels of Caspase-3 and complement C4A than those from controls. The levels of serum APOA-IV in GO patients were significantly higher than those in GD patients and controls. Specific proteins showed elevated expression in tear exosomes from GO patients, indicating that they may play important roles in GO pathogenesis. Frontiers Media S.A. 2022-12-06 /pmc/articles/PMC9763563/ /pubmed/36561758 http://dx.doi.org/10.3389/fimmu.2022.1088606 Text en Copyright © 2022 Shi, Zhao, Xin, Hou, Wang, Xie, Li and Yang https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Immunology Shi, Ting-Ting Zhao, Ru-Xuan Xin, Zhong Hou, Zhi-Jia Wang, Hua Xie, Rong-Rong Li, Dong-Mei Yang, Jin-Kui Tear-derived exosomal biomarkers of Graves’ ophthalmopathy |
title | Tear-derived exosomal biomarkers of Graves’ ophthalmopathy |
title_full | Tear-derived exosomal biomarkers of Graves’ ophthalmopathy |
title_fullStr | Tear-derived exosomal biomarkers of Graves’ ophthalmopathy |
title_full_unstemmed | Tear-derived exosomal biomarkers of Graves’ ophthalmopathy |
title_short | Tear-derived exosomal biomarkers of Graves’ ophthalmopathy |
title_sort | tear-derived exosomal biomarkers of graves’ ophthalmopathy |
topic | Immunology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9763563/ https://www.ncbi.nlm.nih.gov/pubmed/36561758 http://dx.doi.org/10.3389/fimmu.2022.1088606 |
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