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Production and purification of recombinant monoclonal antibodies from human cells based on a primary sequence
There are challenges to using commercially available antibodies generated in animals, including concerns with reproducibility, high costs, and ethical issues. Here, we present a protocol for generating and purifying recombinant antibodies from human HEK293 suspension culture cells from a primary seq...
| Autores principales: | , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Elsevier
2022
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9763751/ https://www.ncbi.nlm.nih.gov/pubmed/36595892 http://dx.doi.org/10.1016/j.xpro.2022.101915 |