Protocol to colonize gnotobiotic mice in early life and assess the impact on early life immune programming

Understanding how changes in gut microbiota in early life impact immune programming can be difficult to study due to variations in the assembly of the microbiota. In this protocol, we describe how to colonize gnotobiotic/germ-free mice in early life with different microbiota community types (e.g., P...

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Detalles Bibliográficos
Autores principales: Lynn, Miriam A., Ryan, Feargal J., Tee, Yee Chern, Lynn, David J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9763935/
https://www.ncbi.nlm.nih.gov/pubmed/36595955
http://dx.doi.org/10.1016/j.xpro.2022.101914
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author Lynn, Miriam A.
Ryan, Feargal J.
Tee, Yee Chern
Lynn, David J.
author_facet Lynn, Miriam A.
Ryan, Feargal J.
Tee, Yee Chern
Lynn, David J.
author_sort Lynn, Miriam A.
collection PubMed
description Understanding how changes in gut microbiota in early life impact immune programming can be difficult to study due to variations in the assembly of the microbiota. In this protocol, we describe how to colonize gnotobiotic/germ-free mice in early life with different microbiota community types (e.g., PAMI and PAMII). We detail several assays to determine whether differential colonization alters immune programming in early life. We also describe how to propagate mouse fecal microbiota transplant material if the donor fecal sample is limited. For complete details on the use and execution of this protocol, please refer to Lynn et al. (2021).(1)
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spelling pubmed-97639352022-12-21 Protocol to colonize gnotobiotic mice in early life and assess the impact on early life immune programming Lynn, Miriam A. Ryan, Feargal J. Tee, Yee Chern Lynn, David J. STAR Protoc Protocol Understanding how changes in gut microbiota in early life impact immune programming can be difficult to study due to variations in the assembly of the microbiota. In this protocol, we describe how to colonize gnotobiotic/germ-free mice in early life with different microbiota community types (e.g., PAMI and PAMII). We detail several assays to determine whether differential colonization alters immune programming in early life. We also describe how to propagate mouse fecal microbiota transplant material if the donor fecal sample is limited. For complete details on the use and execution of this protocol, please refer to Lynn et al. (2021).(1) Elsevier 2022-12-09 /pmc/articles/PMC9763935/ /pubmed/36595955 http://dx.doi.org/10.1016/j.xpro.2022.101914 Text en © 2022 The Author(s) https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Protocol
Lynn, Miriam A.
Ryan, Feargal J.
Tee, Yee Chern
Lynn, David J.
Protocol to colonize gnotobiotic mice in early life and assess the impact on early life immune programming
title Protocol to colonize gnotobiotic mice in early life and assess the impact on early life immune programming
title_full Protocol to colonize gnotobiotic mice in early life and assess the impact on early life immune programming
title_fullStr Protocol to colonize gnotobiotic mice in early life and assess the impact on early life immune programming
title_full_unstemmed Protocol to colonize gnotobiotic mice in early life and assess the impact on early life immune programming
title_short Protocol to colonize gnotobiotic mice in early life and assess the impact on early life immune programming
title_sort protocol to colonize gnotobiotic mice in early life and assess the impact on early life immune programming
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9763935/
https://www.ncbi.nlm.nih.gov/pubmed/36595955
http://dx.doi.org/10.1016/j.xpro.2022.101914
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