Identification of spermatogenesis-associated changes in DNA methylation induced by maternal exposure to chemicals in male germ cells

It is now recognized that maternal environmental factors, including chemical exposure and nutritional conditions, alter DNA methylation patterns in fetal germ cells, subsequently affecting germ cell development as well as offspring phenotypes. Here, we describe steps for detecting DNA methylation ch...

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Detalles Bibliográficos
Autores principales: Tando, Yukiko, Matsui, Yasuhisa
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9763944/
https://www.ncbi.nlm.nih.gov/pubmed/36595925
http://dx.doi.org/10.1016/j.xpro.2022.101912
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author Tando, Yukiko
Matsui, Yasuhisa
author_facet Tando, Yukiko
Matsui, Yasuhisa
author_sort Tando, Yukiko
collection PubMed
description It is now recognized that maternal environmental factors, including chemical exposure and nutritional conditions, alter DNA methylation patterns in fetal germ cells, subsequently affecting germ cell development as well as offspring phenotypes. Here, we describe steps for detecting DNA methylation changes in mouse germ cells isolated from both embryonic and spermatogenic stages after maternal exposure to a chemical compound. For complete details on the use and execution of this protocol, please refer to Tando et al. (2021).(1)
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spelling pubmed-97639442022-12-21 Identification of spermatogenesis-associated changes in DNA methylation induced by maternal exposure to chemicals in male germ cells Tando, Yukiko Matsui, Yasuhisa STAR Protoc Protocol It is now recognized that maternal environmental factors, including chemical exposure and nutritional conditions, alter DNA methylation patterns in fetal germ cells, subsequently affecting germ cell development as well as offspring phenotypes. Here, we describe steps for detecting DNA methylation changes in mouse germ cells isolated from both embryonic and spermatogenic stages after maternal exposure to a chemical compound. For complete details on the use and execution of this protocol, please refer to Tando et al. (2021).(1) Elsevier 2022-12-09 /pmc/articles/PMC9763944/ /pubmed/36595925 http://dx.doi.org/10.1016/j.xpro.2022.101912 Text en © 2022 The Author(s) https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Protocol
Tando, Yukiko
Matsui, Yasuhisa
Identification of spermatogenesis-associated changes in DNA methylation induced by maternal exposure to chemicals in male germ cells
title Identification of spermatogenesis-associated changes in DNA methylation induced by maternal exposure to chemicals in male germ cells
title_full Identification of spermatogenesis-associated changes in DNA methylation induced by maternal exposure to chemicals in male germ cells
title_fullStr Identification of spermatogenesis-associated changes in DNA methylation induced by maternal exposure to chemicals in male germ cells
title_full_unstemmed Identification of spermatogenesis-associated changes in DNA methylation induced by maternal exposure to chemicals in male germ cells
title_short Identification of spermatogenesis-associated changes in DNA methylation induced by maternal exposure to chemicals in male germ cells
title_sort identification of spermatogenesis-associated changes in dna methylation induced by maternal exposure to chemicals in male germ cells
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9763944/
https://www.ncbi.nlm.nih.gov/pubmed/36595925
http://dx.doi.org/10.1016/j.xpro.2022.101912
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