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Establishment of quantitative nested-PCR of Abelson interactor 1 transcript variant-11
Abelson interactor 1 (ABI1), which presents 18 Transcript Variants (TSV), plays an important role in CRC metastasis. Different ABI1-TSVs play synergistic or antagonistic roles in the same pathophysiological events. ABI1 Transcript Variant-11 (ABI1-TSV-11) functionally promotes lymph node metastasis...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9764186/ https://www.ncbi.nlm.nih.gov/pubmed/36561701 http://dx.doi.org/10.1016/j.heliyon.2022.e12119 |
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author | Lin, Tingru Wu, Na Guo, Jingzhu Li, Mei Zhong, Zhaohui Yu, Weidong |
author_facet | Lin, Tingru Wu, Na Guo, Jingzhu Li, Mei Zhong, Zhaohui Yu, Weidong |
author_sort | Lin, Tingru |
collection | PubMed |
description | Abelson interactor 1 (ABI1), which presents 18 Transcript Variants (TSV), plays an important role in CRC metastasis. Different ABI1-TSVs play synergistic or antagonistic roles in the same pathophysiological events. ABI1 Transcript Variant-11 (ABI1-TSV-11) functionally promotes lymph node metastasis of left-sided colorectal cancer (LsCC) and is an independent molecular marker to evaluate the prognosis of patients with LsCC. However, there is still lack of a quick and accurate method to detect the expression of ABI-TSV-11, distinguishing ABI1-TSV-11 from other 17 TSVs. To establish a rapid method specific for ABI1-TSV-11detection, we developed a quantitative nested-PCR method composed of pre-amplification regular PCR using ABI1 universal primer pair and the followed Real Time (RT)-qPCR using ABI1-TSV-11 specific primer pair spanning exon-exon junction. ABI1-TSV-11-overexpressed SW480 and LoVo cell lines were used to verify the quantitative nested-PCR assay, and the sequencing data was used to evaluate the accuracy of ABI1-TSV-11 quantitative nested-PCR assay. The detection limit was 5.24×10(4) copies/ml. ABI1-TSV-11 quantitative nested-PCR provides a new technical means for the detection of ABI1-TSV-11. |
format | Online Article Text |
id | pubmed-9764186 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Elsevier |
record_format | MEDLINE/PubMed |
spelling | pubmed-97641862022-12-21 Establishment of quantitative nested-PCR of Abelson interactor 1 transcript variant-11 Lin, Tingru Wu, Na Guo, Jingzhu Li, Mei Zhong, Zhaohui Yu, Weidong Heliyon Research Article Abelson interactor 1 (ABI1), which presents 18 Transcript Variants (TSV), plays an important role in CRC metastasis. Different ABI1-TSVs play synergistic or antagonistic roles in the same pathophysiological events. ABI1 Transcript Variant-11 (ABI1-TSV-11) functionally promotes lymph node metastasis of left-sided colorectal cancer (LsCC) and is an independent molecular marker to evaluate the prognosis of patients with LsCC. However, there is still lack of a quick and accurate method to detect the expression of ABI-TSV-11, distinguishing ABI1-TSV-11 from other 17 TSVs. To establish a rapid method specific for ABI1-TSV-11detection, we developed a quantitative nested-PCR method composed of pre-amplification regular PCR using ABI1 universal primer pair and the followed Real Time (RT)-qPCR using ABI1-TSV-11 specific primer pair spanning exon-exon junction. ABI1-TSV-11-overexpressed SW480 and LoVo cell lines were used to verify the quantitative nested-PCR assay, and the sequencing data was used to evaluate the accuracy of ABI1-TSV-11 quantitative nested-PCR assay. The detection limit was 5.24×10(4) copies/ml. ABI1-TSV-11 quantitative nested-PCR provides a new technical means for the detection of ABI1-TSV-11. Elsevier 2022-12-07 /pmc/articles/PMC9764186/ /pubmed/36561701 http://dx.doi.org/10.1016/j.heliyon.2022.e12119 Text en © 2022 The Author(s) https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). |
spellingShingle | Research Article Lin, Tingru Wu, Na Guo, Jingzhu Li, Mei Zhong, Zhaohui Yu, Weidong Establishment of quantitative nested-PCR of Abelson interactor 1 transcript variant-11 |
title | Establishment of quantitative nested-PCR of Abelson interactor 1 transcript variant-11 |
title_full | Establishment of quantitative nested-PCR of Abelson interactor 1 transcript variant-11 |
title_fullStr | Establishment of quantitative nested-PCR of Abelson interactor 1 transcript variant-11 |
title_full_unstemmed | Establishment of quantitative nested-PCR of Abelson interactor 1 transcript variant-11 |
title_short | Establishment of quantitative nested-PCR of Abelson interactor 1 transcript variant-11 |
title_sort | establishment of quantitative nested-pcr of abelson interactor 1 transcript variant-11 |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9764186/ https://www.ncbi.nlm.nih.gov/pubmed/36561701 http://dx.doi.org/10.1016/j.heliyon.2022.e12119 |
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