Cargando…

Establishment of quantitative nested-PCR of Abelson interactor 1 transcript variant-11

Abelson interactor 1 (ABI1), which presents 18 Transcript Variants (TSV), plays an important role in CRC metastasis. Different ABI1-TSVs play synergistic or antagonistic roles in the same pathophysiological events. ABI1 Transcript Variant-11 (ABI1-TSV-11) functionally promotes lymph node metastasis...

Descripción completa

Detalles Bibliográficos
Autores principales: Lin, Tingru, Wu, Na, Guo, Jingzhu, Li, Mei, Zhong, Zhaohui, Yu, Weidong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9764186/
https://www.ncbi.nlm.nih.gov/pubmed/36561701
http://dx.doi.org/10.1016/j.heliyon.2022.e12119
_version_ 1784853222206210048
author Lin, Tingru
Wu, Na
Guo, Jingzhu
Li, Mei
Zhong, Zhaohui
Yu, Weidong
author_facet Lin, Tingru
Wu, Na
Guo, Jingzhu
Li, Mei
Zhong, Zhaohui
Yu, Weidong
author_sort Lin, Tingru
collection PubMed
description Abelson interactor 1 (ABI1), which presents 18 Transcript Variants (TSV), plays an important role in CRC metastasis. Different ABI1-TSVs play synergistic or antagonistic roles in the same pathophysiological events. ABI1 Transcript Variant-11 (ABI1-TSV-11) functionally promotes lymph node metastasis of left-sided colorectal cancer (LsCC) and is an independent molecular marker to evaluate the prognosis of patients with LsCC. However, there is still lack of a quick and accurate method to detect the expression of ABI-TSV-11, distinguishing ABI1-TSV-11 from other 17 TSVs. To establish a rapid method specific for ABI1-TSV-11detection, we developed a quantitative nested-PCR method composed of pre-amplification regular PCR using ABI1 universal primer pair and the followed Real Time (RT)-qPCR using ABI1-TSV-11 specific primer pair spanning exon-exon junction. ABI1-TSV-11-overexpressed SW480 and LoVo cell lines were used to verify the quantitative nested-PCR assay, and the sequencing data was used to evaluate the accuracy of ABI1-TSV-11 quantitative nested-PCR assay. The detection limit was 5.24×10(4) copies/ml. ABI1-TSV-11 quantitative nested-PCR provides a new technical means for the detection of ABI1-TSV-11.
format Online
Article
Text
id pubmed-9764186
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher Elsevier
record_format MEDLINE/PubMed
spelling pubmed-97641862022-12-21 Establishment of quantitative nested-PCR of Abelson interactor 1 transcript variant-11 Lin, Tingru Wu, Na Guo, Jingzhu Li, Mei Zhong, Zhaohui Yu, Weidong Heliyon Research Article Abelson interactor 1 (ABI1), which presents 18 Transcript Variants (TSV), plays an important role in CRC metastasis. Different ABI1-TSVs play synergistic or antagonistic roles in the same pathophysiological events. ABI1 Transcript Variant-11 (ABI1-TSV-11) functionally promotes lymph node metastasis of left-sided colorectal cancer (LsCC) and is an independent molecular marker to evaluate the prognosis of patients with LsCC. However, there is still lack of a quick and accurate method to detect the expression of ABI-TSV-11, distinguishing ABI1-TSV-11 from other 17 TSVs. To establish a rapid method specific for ABI1-TSV-11detection, we developed a quantitative nested-PCR method composed of pre-amplification regular PCR using ABI1 universal primer pair and the followed Real Time (RT)-qPCR using ABI1-TSV-11 specific primer pair spanning exon-exon junction. ABI1-TSV-11-overexpressed SW480 and LoVo cell lines were used to verify the quantitative nested-PCR assay, and the sequencing data was used to evaluate the accuracy of ABI1-TSV-11 quantitative nested-PCR assay. The detection limit was 5.24×10(4) copies/ml. ABI1-TSV-11 quantitative nested-PCR provides a new technical means for the detection of ABI1-TSV-11. Elsevier 2022-12-07 /pmc/articles/PMC9764186/ /pubmed/36561701 http://dx.doi.org/10.1016/j.heliyon.2022.e12119 Text en © 2022 The Author(s) https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Research Article
Lin, Tingru
Wu, Na
Guo, Jingzhu
Li, Mei
Zhong, Zhaohui
Yu, Weidong
Establishment of quantitative nested-PCR of Abelson interactor 1 transcript variant-11
title Establishment of quantitative nested-PCR of Abelson interactor 1 transcript variant-11
title_full Establishment of quantitative nested-PCR of Abelson interactor 1 transcript variant-11
title_fullStr Establishment of quantitative nested-PCR of Abelson interactor 1 transcript variant-11
title_full_unstemmed Establishment of quantitative nested-PCR of Abelson interactor 1 transcript variant-11
title_short Establishment of quantitative nested-PCR of Abelson interactor 1 transcript variant-11
title_sort establishment of quantitative nested-pcr of abelson interactor 1 transcript variant-11
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9764186/
https://www.ncbi.nlm.nih.gov/pubmed/36561701
http://dx.doi.org/10.1016/j.heliyon.2022.e12119
work_keys_str_mv AT lintingru establishmentofquantitativenestedpcrofabelsoninteractor1transcriptvariant11
AT wuna establishmentofquantitativenestedpcrofabelsoninteractor1transcriptvariant11
AT guojingzhu establishmentofquantitativenestedpcrofabelsoninteractor1transcriptvariant11
AT limei establishmentofquantitativenestedpcrofabelsoninteractor1transcriptvariant11
AT zhongzhaohui establishmentofquantitativenestedpcrofabelsoninteractor1transcriptvariant11
AT yuweidong establishmentofquantitativenestedpcrofabelsoninteractor1transcriptvariant11