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Acoustic sorting of microfluidic droplets at kHz rates using optical absorbance

Droplet microfluidics allows one to address the ever-increasing demand to screen large libraries of biological samples. Absorbance spectroscopy complements the golden standard of fluorescence detection by label free target identification and providing more quantifiable data. However, this is limited...

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Autores principales: Richter, Esther S., Link, Andreas, McGrath, John S., Sparrow, Raymond W., Gantz, Maximilian, Medcalf, Elliot J., Hollfelder, Florian, Franke, Thomas
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Royal Society of Chemistry 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9764809/
https://www.ncbi.nlm.nih.gov/pubmed/36472476
http://dx.doi.org/10.1039/d2lc00871h
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author Richter, Esther S.
Link, Andreas
McGrath, John S.
Sparrow, Raymond W.
Gantz, Maximilian
Medcalf, Elliot J.
Hollfelder, Florian
Franke, Thomas
author_facet Richter, Esther S.
Link, Andreas
McGrath, John S.
Sparrow, Raymond W.
Gantz, Maximilian
Medcalf, Elliot J.
Hollfelder, Florian
Franke, Thomas
author_sort Richter, Esther S.
collection PubMed
description Droplet microfluidics allows one to address the ever-increasing demand to screen large libraries of biological samples. Absorbance spectroscopy complements the golden standard of fluorescence detection by label free target identification and providing more quantifiable data. However, this is limited by speed and sensitivity. In this paper we increase the speed of sorting by including acoustofluidics, achieving sorting rates of target droplets of 1 kHz. We improved the device design for detection of absorbance using fibre-based interrogation of samples with integrated lenses in the microfluidic PDMS device for focusing and collimation of light. This optical improvement reduces the scattering and refraction artefacts, improving the signal quality and sensitivity. The novel design allows us to overcome limitations based on dielectrophoresis sorting, such as droplet size dependency, material and dielectric properties of samples. Our acoustic activated absorbance sorter removes the need for offset dyes or matching oils and sorts about a magnitude faster than current absorbance sorters.
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spelling pubmed-97648092023-01-04 Acoustic sorting of microfluidic droplets at kHz rates using optical absorbance Richter, Esther S. Link, Andreas McGrath, John S. Sparrow, Raymond W. Gantz, Maximilian Medcalf, Elliot J. Hollfelder, Florian Franke, Thomas Lab Chip Chemistry Droplet microfluidics allows one to address the ever-increasing demand to screen large libraries of biological samples. Absorbance spectroscopy complements the golden standard of fluorescence detection by label free target identification and providing more quantifiable data. However, this is limited by speed and sensitivity. In this paper we increase the speed of sorting by including acoustofluidics, achieving sorting rates of target droplets of 1 kHz. We improved the device design for detection of absorbance using fibre-based interrogation of samples with integrated lenses in the microfluidic PDMS device for focusing and collimation of light. This optical improvement reduces the scattering and refraction artefacts, improving the signal quality and sensitivity. The novel design allows us to overcome limitations based on dielectrophoresis sorting, such as droplet size dependency, material and dielectric properties of samples. Our acoustic activated absorbance sorter removes the need for offset dyes or matching oils and sorts about a magnitude faster than current absorbance sorters. The Royal Society of Chemistry 2022-12-01 /pmc/articles/PMC9764809/ /pubmed/36472476 http://dx.doi.org/10.1039/d2lc00871h Text en This journal is © The Royal Society of Chemistry https://creativecommons.org/licenses/by/3.0/
spellingShingle Chemistry
Richter, Esther S.
Link, Andreas
McGrath, John S.
Sparrow, Raymond W.
Gantz, Maximilian
Medcalf, Elliot J.
Hollfelder, Florian
Franke, Thomas
Acoustic sorting of microfluidic droplets at kHz rates using optical absorbance
title Acoustic sorting of microfluidic droplets at kHz rates using optical absorbance
title_full Acoustic sorting of microfluidic droplets at kHz rates using optical absorbance
title_fullStr Acoustic sorting of microfluidic droplets at kHz rates using optical absorbance
title_full_unstemmed Acoustic sorting of microfluidic droplets at kHz rates using optical absorbance
title_short Acoustic sorting of microfluidic droplets at kHz rates using optical absorbance
title_sort acoustic sorting of microfluidic droplets at khz rates using optical absorbance
topic Chemistry
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9764809/
https://www.ncbi.nlm.nih.gov/pubmed/36472476
http://dx.doi.org/10.1039/d2lc00871h
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