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A Cristae-Like Microcompartment in Desulfobacterota

Some Alphaproteobacteria contain intracytoplasmic membranes (ICMs) and proteins homologous to those responsible for the mitochondrial cristae, an observation which has given rise to the hypothesis that the Alphaproteobacteria endosymbiont had already evolved cristae-like structures and functions. Ho...

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Autores principales: McGlynn, Shawn Erin, Perkins, Guy, Sim, Min Sub, Mackey, Mason, Deerinck, Thomas J., Thor, Andrea, Phan, Sebastien, Ballard, Daniel, Ellisman, Mark H., Orphan, Victoria J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Society for Microbiology 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9764997/
https://www.ncbi.nlm.nih.gov/pubmed/36321837
http://dx.doi.org/10.1128/mbio.01613-22
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author McGlynn, Shawn Erin
Perkins, Guy
Sim, Min Sub
Mackey, Mason
Deerinck, Thomas J.
Thor, Andrea
Phan, Sebastien
Ballard, Daniel
Ellisman, Mark H.
Orphan, Victoria J.
author_facet McGlynn, Shawn Erin
Perkins, Guy
Sim, Min Sub
Mackey, Mason
Deerinck, Thomas J.
Thor, Andrea
Phan, Sebastien
Ballard, Daniel
Ellisman, Mark H.
Orphan, Victoria J.
author_sort McGlynn, Shawn Erin
collection PubMed
description Some Alphaproteobacteria contain intracytoplasmic membranes (ICMs) and proteins homologous to those responsible for the mitochondrial cristae, an observation which has given rise to the hypothesis that the Alphaproteobacteria endosymbiont had already evolved cristae-like structures and functions. However, our knowledge of microbial fine structure is still limited, leaving open the possibility of structurally homologous ICMs outside the Alphaproteobacteria. Here, we report on the detailed characterization of lamellar cristae-like ICMs in environmental sulfate-reducing Desulfobacterota that form syntrophic partnerships with anaerobic methane-oxidizing (ANME) archaea. These structures are junction-bound to the cytoplasmic membrane and resemble the form seen in the lamellar cristae of opisthokont mitochondria. Extending these observations, we also characterized similar structures in Desulfovibrio carbinolicus, a close relative of the magnetotactic D. magneticus, which does not contain magnetosomes. Despite a remarkable structural similarity, the key proteins involved in cristae formation have not yet been identified in Desulfobacterota, suggesting that an analogous, but not a homologous, protein organization system developed during the evolution of some members of Desulfobacterota.
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spelling pubmed-97649972022-12-21 A Cristae-Like Microcompartment in Desulfobacterota McGlynn, Shawn Erin Perkins, Guy Sim, Min Sub Mackey, Mason Deerinck, Thomas J. Thor, Andrea Phan, Sebastien Ballard, Daniel Ellisman, Mark H. Orphan, Victoria J. mBio Research Article Some Alphaproteobacteria contain intracytoplasmic membranes (ICMs) and proteins homologous to those responsible for the mitochondrial cristae, an observation which has given rise to the hypothesis that the Alphaproteobacteria endosymbiont had already evolved cristae-like structures and functions. However, our knowledge of microbial fine structure is still limited, leaving open the possibility of structurally homologous ICMs outside the Alphaproteobacteria. Here, we report on the detailed characterization of lamellar cristae-like ICMs in environmental sulfate-reducing Desulfobacterota that form syntrophic partnerships with anaerobic methane-oxidizing (ANME) archaea. These structures are junction-bound to the cytoplasmic membrane and resemble the form seen in the lamellar cristae of opisthokont mitochondria. Extending these observations, we also characterized similar structures in Desulfovibrio carbinolicus, a close relative of the magnetotactic D. magneticus, which does not contain magnetosomes. Despite a remarkable structural similarity, the key proteins involved in cristae formation have not yet been identified in Desulfobacterota, suggesting that an analogous, but not a homologous, protein organization system developed during the evolution of some members of Desulfobacterota. American Society for Microbiology 2022-11-02 /pmc/articles/PMC9764997/ /pubmed/36321837 http://dx.doi.org/10.1128/mbio.01613-22 Text en Copyright © 2022 McGlynn et al. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Research Article
McGlynn, Shawn Erin
Perkins, Guy
Sim, Min Sub
Mackey, Mason
Deerinck, Thomas J.
Thor, Andrea
Phan, Sebastien
Ballard, Daniel
Ellisman, Mark H.
Orphan, Victoria J.
A Cristae-Like Microcompartment in Desulfobacterota
title A Cristae-Like Microcompartment in Desulfobacterota
title_full A Cristae-Like Microcompartment in Desulfobacterota
title_fullStr A Cristae-Like Microcompartment in Desulfobacterota
title_full_unstemmed A Cristae-Like Microcompartment in Desulfobacterota
title_short A Cristae-Like Microcompartment in Desulfobacterota
title_sort cristae-like microcompartment in desulfobacterota
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9764997/
https://www.ncbi.nlm.nih.gov/pubmed/36321837
http://dx.doi.org/10.1128/mbio.01613-22
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