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Extracellular Vesicles Contribute to Mixed-Fungal Species Competition during Biofilm Initiation
Extracellular vesicles commonly modulate interactions among cellular communities. Recent studies demonstrate that biofilm maturation features, including matrix production, drug resistance, and dispersion, require the delivery of a core protein and carbohydrate vesicle cargo in Candida species. The f...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
American Society for Microbiology
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9765065/ https://www.ncbi.nlm.nih.gov/pubmed/36377868 http://dx.doi.org/10.1128/mbio.02988-22 |
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author | Zarnowski, Robert Massey, Justin Mitchell, Aaron P. Andes, David |
author_facet | Zarnowski, Robert Massey, Justin Mitchell, Aaron P. Andes, David |
author_sort | Zarnowski, Robert |
collection | PubMed |
description | Extracellular vesicles commonly modulate interactions among cellular communities. Recent studies demonstrate that biofilm maturation features, including matrix production, drug resistance, and dispersion, require the delivery of a core protein and carbohydrate vesicle cargo in Candida species. The function of the vesicle cargo for these advanced-phase biofilm characteristics appears to be conserved across Candida species. Mixed-species interactions in mature biofilms indicate that vesicle cargo serves a cooperative role in preserving the community. Here, we define the function of biofilm-associated vesicles for biofilm initiation both within and among five species across the Candida genus. We found similar vesicle cargo functions for several conserved proteins across species, based on the behavior of mutants. Repletion of the adhesion environment with wild-type vesicles returned the community phenotype toward reference levels in intraspecies experiments. However, cross-species vesicle complementation did not restore the wild-type biology and in fact drove the phenotype in the opposite direction for most cross-species interactions. Further study of mixed-species biofilm adhesion and exogenous wild-type vesicle administration similarly demonstrated competitive interactions. Our studies indicate that similar vesicle cargoes contribute to biofilm initiation. However, vesicles from disparate species serve an interference competitive role in mixed-Candida species scenarios. |
format | Online Article Text |
id | pubmed-9765065 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | American Society for Microbiology |
record_format | MEDLINE/PubMed |
spelling | pubmed-97650652022-12-21 Extracellular Vesicles Contribute to Mixed-Fungal Species Competition during Biofilm Initiation Zarnowski, Robert Massey, Justin Mitchell, Aaron P. Andes, David mBio Observation Extracellular vesicles commonly modulate interactions among cellular communities. Recent studies demonstrate that biofilm maturation features, including matrix production, drug resistance, and dispersion, require the delivery of a core protein and carbohydrate vesicle cargo in Candida species. The function of the vesicle cargo for these advanced-phase biofilm characteristics appears to be conserved across Candida species. Mixed-species interactions in mature biofilms indicate that vesicle cargo serves a cooperative role in preserving the community. Here, we define the function of biofilm-associated vesicles for biofilm initiation both within and among five species across the Candida genus. We found similar vesicle cargo functions for several conserved proteins across species, based on the behavior of mutants. Repletion of the adhesion environment with wild-type vesicles returned the community phenotype toward reference levels in intraspecies experiments. However, cross-species vesicle complementation did not restore the wild-type biology and in fact drove the phenotype in the opposite direction for most cross-species interactions. Further study of mixed-species biofilm adhesion and exogenous wild-type vesicle administration similarly demonstrated competitive interactions. Our studies indicate that similar vesicle cargoes contribute to biofilm initiation. However, vesicles from disparate species serve an interference competitive role in mixed-Candida species scenarios. American Society for Microbiology 2022-11-15 /pmc/articles/PMC9765065/ /pubmed/36377868 http://dx.doi.org/10.1128/mbio.02988-22 Text en Copyright © 2022 Zarnowski et al. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license (https://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Observation Zarnowski, Robert Massey, Justin Mitchell, Aaron P. Andes, David Extracellular Vesicles Contribute to Mixed-Fungal Species Competition during Biofilm Initiation |
title | Extracellular Vesicles Contribute to Mixed-Fungal Species Competition during Biofilm Initiation |
title_full | Extracellular Vesicles Contribute to Mixed-Fungal Species Competition during Biofilm Initiation |
title_fullStr | Extracellular Vesicles Contribute to Mixed-Fungal Species Competition during Biofilm Initiation |
title_full_unstemmed | Extracellular Vesicles Contribute to Mixed-Fungal Species Competition during Biofilm Initiation |
title_short | Extracellular Vesicles Contribute to Mixed-Fungal Species Competition during Biofilm Initiation |
title_sort | extracellular vesicles contribute to mixed-fungal species competition during biofilm initiation |
topic | Observation |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9765065/ https://www.ncbi.nlm.nih.gov/pubmed/36377868 http://dx.doi.org/10.1128/mbio.02988-22 |
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