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The RNA-Binding Protein ProQ Promotes Antibiotic Persistence in Salmonella

Bacterial populations can survive exposure to antibiotics through transient phenotypic and gene expression changes. These changes can be attributed to a small subpopulation of bacteria, giving rise to antibiotic persistence. Although this phenomenon has been known for decades, much remains to be lea...

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Autores principales: Rizvanovic, Alisa, Michaux, Charlotte, Panza, Margherita, Iloglu, Zeynep, Helaine, Sophie, Wagner, E. Gerhart H., Holmqvist, Erik
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Society for Microbiology 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9765298/
https://www.ncbi.nlm.nih.gov/pubmed/36409088
http://dx.doi.org/10.1128/mbio.02891-22
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author Rizvanovic, Alisa
Michaux, Charlotte
Panza, Margherita
Iloglu, Zeynep
Helaine, Sophie
Wagner, E. Gerhart H.
Holmqvist, Erik
author_facet Rizvanovic, Alisa
Michaux, Charlotte
Panza, Margherita
Iloglu, Zeynep
Helaine, Sophie
Wagner, E. Gerhart H.
Holmqvist, Erik
author_sort Rizvanovic, Alisa
collection PubMed
description Bacterial populations can survive exposure to antibiotics through transient phenotypic and gene expression changes. These changes can be attributed to a small subpopulation of bacteria, giving rise to antibiotic persistence. Although this phenomenon has been known for decades, much remains to be learned about the mechanisms that drive persister formation. The RNA-binding protein ProQ has recently emerged as a global regulator of gene expression. Here, we show that ProQ impacts persister formation in Salmonella. In vitro, ProQ contributes to growth arrest in a subset of cells that are able to survive treatment at high concentrations of different antibiotics. The underlying mechanism for ProQ-dependent persister formation involves the activation of metabolically costly processes, including the flagellar pathway and the type III protein secretion system encoded on Salmonella pathogenicity island 2. Importantly, we show that the ProQ-dependent phenotype is relevant during macrophage infection and allows Salmonella to survive the combined action of host immune defenses and antibiotics. Together, our data highlight the importance of ProQ in Salmonella persistence and pathogenesis.
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spelling pubmed-97652982022-12-21 The RNA-Binding Protein ProQ Promotes Antibiotic Persistence in Salmonella Rizvanovic, Alisa Michaux, Charlotte Panza, Margherita Iloglu, Zeynep Helaine, Sophie Wagner, E. Gerhart H. Holmqvist, Erik mBio Research Article Bacterial populations can survive exposure to antibiotics through transient phenotypic and gene expression changes. These changes can be attributed to a small subpopulation of bacteria, giving rise to antibiotic persistence. Although this phenomenon has been known for decades, much remains to be learned about the mechanisms that drive persister formation. The RNA-binding protein ProQ has recently emerged as a global regulator of gene expression. Here, we show that ProQ impacts persister formation in Salmonella. In vitro, ProQ contributes to growth arrest in a subset of cells that are able to survive treatment at high concentrations of different antibiotics. The underlying mechanism for ProQ-dependent persister formation involves the activation of metabolically costly processes, including the flagellar pathway and the type III protein secretion system encoded on Salmonella pathogenicity island 2. Importantly, we show that the ProQ-dependent phenotype is relevant during macrophage infection and allows Salmonella to survive the combined action of host immune defenses and antibiotics. Together, our data highlight the importance of ProQ in Salmonella persistence and pathogenesis. American Society for Microbiology 2022-11-21 /pmc/articles/PMC9765298/ /pubmed/36409088 http://dx.doi.org/10.1128/mbio.02891-22 Text en Copyright © 2022 Rizvanovic et al. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Research Article
Rizvanovic, Alisa
Michaux, Charlotte
Panza, Margherita
Iloglu, Zeynep
Helaine, Sophie
Wagner, E. Gerhart H.
Holmqvist, Erik
The RNA-Binding Protein ProQ Promotes Antibiotic Persistence in Salmonella
title The RNA-Binding Protein ProQ Promotes Antibiotic Persistence in Salmonella
title_full The RNA-Binding Protein ProQ Promotes Antibiotic Persistence in Salmonella
title_fullStr The RNA-Binding Protein ProQ Promotes Antibiotic Persistence in Salmonella
title_full_unstemmed The RNA-Binding Protein ProQ Promotes Antibiotic Persistence in Salmonella
title_short The RNA-Binding Protein ProQ Promotes Antibiotic Persistence in Salmonella
title_sort rna-binding protein proq promotes antibiotic persistence in salmonella
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9765298/
https://www.ncbi.nlm.nih.gov/pubmed/36409088
http://dx.doi.org/10.1128/mbio.02891-22
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