Tuning of Gene Expression in Clostridium phytofermentans Using Synthetic Promoters and CRISPRi

[Image: see text] Control of gene expression is fundamental to cell engineering. Here we demonstrate a set of approaches to tune gene expression in Clostridia using the model Clostridium phytofermentans. Initially, we develop a simple benchtop electroporation method that we use to identify a set of...

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Detalles Bibliográficos
Autores principales: Rostain, William, Zaplana, Tom, Boutard, Magali, Baum, Chloé, Tabuteau, Sibylle, Sanitha, Mary, Ramya, Mohandass, Guss, Adam, Ettwiller, Laurence, Tolonen, Andrew C.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Chemical Society 2022
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9765743/
https://www.ncbi.nlm.nih.gov/pubmed/36427328
http://dx.doi.org/10.1021/acssynbio.2c00385

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