Isolation and characterization of a SARS-CoV-2 variant with a Q677H mutation in the spike protein

We isolated 20 SARS-CoV-2 strains from positive clinical samples collected in Columbus, Ohio, and investigated the replication of one pair of isolates: a clade 20G strain and a variant of this strain carrying a Q677H mutation in the spike protein and six other amino acid mutations. The OSU.20G varia...

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Detalles Bibliográficos
Autores principales: Niu, Xiaoyu, Xu, Jiayu, Liu, Mingde, Tu, Huolin, Koenig, Sara N., Saif, Linda J., Jones, Daniel M., Wang, Qiuhong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Vienna 2022
Materias:
Brief Report
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9767398/
https://www.ncbi.nlm.nih.gov/pubmed/36539656
http://dx.doi.org/10.1007/s00705-022-05621-5
Descripción
Sumario:We isolated 20 SARS-CoV-2 strains from positive clinical samples collected in Columbus, Ohio, and investigated the replication of one pair of isolates: a clade 20G strain and a variant of this strain carrying a Q677H mutation in the spike protein and six other amino acid mutations. The OSU.20G variant replicated to a higher peak infectious titer than the 20G base strain in Vero-E6 cells, but the titers were similar when both strains were grown in Calu-3 cells. These results suggest that the OSU.20G variant has increased replication fitness compared to the 20G base strain. This may have contributed to its emergence in December 2020‐January 2021. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1007/s00705-022-05621-5.

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