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Isolation and characterization of a SARS-CoV-2 variant with a Q677H mutation in the spike protein

We isolated 20 SARS-CoV-2 strains from positive clinical samples collected in Columbus, Ohio, and investigated the replication of one pair of isolates: a clade 20G strain and a variant of this strain carrying a Q677H mutation in the spike protein and six other amino acid mutations. The OSU.20G varia...

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Autores principales: Niu, Xiaoyu, Xu, Jiayu, Liu, Mingde, Tu, Huolin, Koenig, Sara N., Saif, Linda J., Jones, Daniel M., Wang, Qiuhong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Vienna 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9767398/
https://www.ncbi.nlm.nih.gov/pubmed/36539656
http://dx.doi.org/10.1007/s00705-022-05621-5
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author Niu, Xiaoyu
Xu, Jiayu
Liu, Mingde
Tu, Huolin
Koenig, Sara N.
Saif, Linda J.
Jones, Daniel M.
Wang, Qiuhong
author_facet Niu, Xiaoyu
Xu, Jiayu
Liu, Mingde
Tu, Huolin
Koenig, Sara N.
Saif, Linda J.
Jones, Daniel M.
Wang, Qiuhong
author_sort Niu, Xiaoyu
collection PubMed
description We isolated 20 SARS-CoV-2 strains from positive clinical samples collected in Columbus, Ohio, and investigated the replication of one pair of isolates: a clade 20G strain and a variant of this strain carrying a Q677H mutation in the spike protein and six other amino acid mutations. The OSU.20G variant replicated to a higher peak infectious titer than the 20G base strain in Vero-E6 cells, but the titers were similar when both strains were grown in Calu-3 cells. These results suggest that the OSU.20G variant has increased replication fitness compared to the 20G base strain. This may have contributed to its emergence in December 2020‐January 2021. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1007/s00705-022-05621-5.
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spelling pubmed-97673982022-12-21 Isolation and characterization of a SARS-CoV-2 variant with a Q677H mutation in the spike protein Niu, Xiaoyu Xu, Jiayu Liu, Mingde Tu, Huolin Koenig, Sara N. Saif, Linda J. Jones, Daniel M. Wang, Qiuhong Arch Virol Brief Report We isolated 20 SARS-CoV-2 strains from positive clinical samples collected in Columbus, Ohio, and investigated the replication of one pair of isolates: a clade 20G strain and a variant of this strain carrying a Q677H mutation in the spike protein and six other amino acid mutations. The OSU.20G variant replicated to a higher peak infectious titer than the 20G base strain in Vero-E6 cells, but the titers were similar when both strains were grown in Calu-3 cells. These results suggest that the OSU.20G variant has increased replication fitness compared to the 20G base strain. This may have contributed to its emergence in December 2020‐January 2021. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1007/s00705-022-05621-5. Springer Vienna 2022-12-20 2023 /pmc/articles/PMC9767398/ /pubmed/36539656 http://dx.doi.org/10.1007/s00705-022-05621-5 Text en © The Author(s), under exclusive licence to Springer-Verlag GmbH Austria, part of Springer Nature 2022 This article is made available via the PMC Open Access Subset for unrestricted research re-use and secondary analysis in any form or by any means with acknowledgement of the original source. These permissions are granted for the duration of the World Health Organization (WHO) declaration of COVID-19 as a global pandemic.
spellingShingle Brief Report
Niu, Xiaoyu
Xu, Jiayu
Liu, Mingde
Tu, Huolin
Koenig, Sara N.
Saif, Linda J.
Jones, Daniel M.
Wang, Qiuhong
Isolation and characterization of a SARS-CoV-2 variant with a Q677H mutation in the spike protein
title Isolation and characterization of a SARS-CoV-2 variant with a Q677H mutation in the spike protein
title_full Isolation and characterization of a SARS-CoV-2 variant with a Q677H mutation in the spike protein
title_fullStr Isolation and characterization of a SARS-CoV-2 variant with a Q677H mutation in the spike protein
title_full_unstemmed Isolation and characterization of a SARS-CoV-2 variant with a Q677H mutation in the spike protein
title_short Isolation and characterization of a SARS-CoV-2 variant with a Q677H mutation in the spike protein
title_sort isolation and characterization of a sars-cov-2 variant with a q677h mutation in the spike protein
topic Brief Report
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9767398/
https://www.ncbi.nlm.nih.gov/pubmed/36539656
http://dx.doi.org/10.1007/s00705-022-05621-5
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