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Shifting the focus of zebrafish toward a model of the tumor microenvironment
Cancer cells exist in a complex ecosystem with numerous other cell types in the tumor microenvironment (TME). The composition of this tumor/TME ecosystem will vary at each anatomic site and affects phenotypes such as initiation, metastasis, and drug resistance. A mechanistic understanding of the lar...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
eLife Sciences Publications, Ltd
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9767465/ https://www.ncbi.nlm.nih.gov/pubmed/36538362 http://dx.doi.org/10.7554/eLife.69703 |
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author | Weiss, Joshua M Lumaquin-Yin, Dianne Montal, Emily Suresh, Shruthy Leonhardt, Carl S White, Richard M |
author_facet | Weiss, Joshua M Lumaquin-Yin, Dianne Montal, Emily Suresh, Shruthy Leonhardt, Carl S White, Richard M |
author_sort | Weiss, Joshua M |
collection | PubMed |
description | Cancer cells exist in a complex ecosystem with numerous other cell types in the tumor microenvironment (TME). The composition of this tumor/TME ecosystem will vary at each anatomic site and affects phenotypes such as initiation, metastasis, and drug resistance. A mechanistic understanding of the large number of cell-cell interactions between tumor and TME requires models that allow us to both characterize as well as genetically perturb this complexity. Zebrafish are a model system optimized for this problem, because of the large number of existing cell-type-specific drivers that can label nearly any cell in the TME. These include stromal cells, immune cells, and tissue resident normal cells. These cell-type-specific promoters/enhancers can be used to drive fluorophores to facilitate imaging and also CRISPR cassettes to facilitate perturbations. A major advantage of the zebrafish is the ease by which large numbers of TME cell types can be studied at once, within the same animal. While these features make the zebrafish well suited to investigate the TME, the model has important limitations, which we also discuss. In this review, we describe the existing toolset for studying the TME using zebrafish models of cancer and highlight unique biological insights that can be gained by leveraging this powerful resource. |
format | Online Article Text |
id | pubmed-9767465 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | eLife Sciences Publications, Ltd |
record_format | MEDLINE/PubMed |
spelling | pubmed-97674652022-12-21 Shifting the focus of zebrafish toward a model of the tumor microenvironment Weiss, Joshua M Lumaquin-Yin, Dianne Montal, Emily Suresh, Shruthy Leonhardt, Carl S White, Richard M eLife Cancer Biology Cancer cells exist in a complex ecosystem with numerous other cell types in the tumor microenvironment (TME). The composition of this tumor/TME ecosystem will vary at each anatomic site and affects phenotypes such as initiation, metastasis, and drug resistance. A mechanistic understanding of the large number of cell-cell interactions between tumor and TME requires models that allow us to both characterize as well as genetically perturb this complexity. Zebrafish are a model system optimized for this problem, because of the large number of existing cell-type-specific drivers that can label nearly any cell in the TME. These include stromal cells, immune cells, and tissue resident normal cells. These cell-type-specific promoters/enhancers can be used to drive fluorophores to facilitate imaging and also CRISPR cassettes to facilitate perturbations. A major advantage of the zebrafish is the ease by which large numbers of TME cell types can be studied at once, within the same animal. While these features make the zebrafish well suited to investigate the TME, the model has important limitations, which we also discuss. In this review, we describe the existing toolset for studying the TME using zebrafish models of cancer and highlight unique biological insights that can be gained by leveraging this powerful resource. eLife Sciences Publications, Ltd 2022-12-20 /pmc/articles/PMC9767465/ /pubmed/36538362 http://dx.doi.org/10.7554/eLife.69703 Text en © 2022, Weiss, Lumaquin-Yin, Montal et al https://creativecommons.org/licenses/by/4.0/This article is distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use and redistribution provided that the original author and source are credited. |
spellingShingle | Cancer Biology Weiss, Joshua M Lumaquin-Yin, Dianne Montal, Emily Suresh, Shruthy Leonhardt, Carl S White, Richard M Shifting the focus of zebrafish toward a model of the tumor microenvironment |
title | Shifting the focus of zebrafish toward a model of the tumor microenvironment |
title_full | Shifting the focus of zebrafish toward a model of the tumor microenvironment |
title_fullStr | Shifting the focus of zebrafish toward a model of the tumor microenvironment |
title_full_unstemmed | Shifting the focus of zebrafish toward a model of the tumor microenvironment |
title_short | Shifting the focus of zebrafish toward a model of the tumor microenvironment |
title_sort | shifting the focus of zebrafish toward a model of the tumor microenvironment |
topic | Cancer Biology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9767465/ https://www.ncbi.nlm.nih.gov/pubmed/36538362 http://dx.doi.org/10.7554/eLife.69703 |
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