Validity of the Self-administered Food Frequency Questionnaire Used in the 5-year Follow-up Survey for the JPHC Study to Assess Folate, Vitamin B(6) and B(12) Intake: Comparison with Dietary Records and Blood Level

To validate a self-administered food frequency questionnaire (FFQ) for the estimation of dietary intake of folate, vitamins B(6) and B(12), we compared measures of these vitamins by the FFQ and plasma concentrations among 87 male volunteers aged 40-69 years in the Japan Public Health Center-based prospective study (JPHC Study). No men used folate, vitamins B(6) or B(12) as supplements. There was a moderate association between energy-adjusted dietary intake and plasma concentration for folate and vitamin B(6). The Spearman correlation coefficient was 0.26 and 0.23, respectively, in the total samples. These correlation coefficients were slightly higher when men in the Ishikawa PHC area were excluded from the analysis; the correlation coefficient was 0.29 for folate and 0.26 for vitamin B(6). No significant correlation was found between dietary vitamin B(12) intake and serum B(12) concentration; the correlation coefficient was 0.06 in the total sample and 0.15 when the Ishikawa men were excluded from the analysis. In conclusion, we found that the FFQ used for the 5-year follow-up survey of JPHC Study could reasonably rank individuals by dietary intake of folate and vitamin B(6), but not vitamin B(12), among Japanese community-dwelling population samples.