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Mechanism of human Lig1 regulation by PCNA in Okazaki fragment sealing

During lagging strand synthesis, DNA Ligase 1 (Lig1) cooperates with the sliding clamp PCNA to seal the nicks between Okazaki fragments generated by Pol δ and Flap endonuclease 1 (FEN1). We present several cryo-EM structures combined with functional assays, showing that human Lig1 recruits PCNA to n...

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Detalles Bibliográficos
Autores principales:	Blair, Kerry, Tehseen, Muhammad, Raducanu, Vlad-Stefan, Shahid, Taha, Lancey, Claudia, Rashid, Fahad, Crehuet, Ramon, Hamdan, Samir M., De Biasio, Alfredo
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Nature Publishing Group UK 2022
Materias:	Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9767926/ https://www.ncbi.nlm.nih.gov/pubmed/36539424 http://dx.doi.org/10.1038/s41467-022-35475-z

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