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Localization and potential role of prostate microbiota

INTRODUCTION: We aimed to clarify the presence and localization of the prostate microbiota and examine its association with benign prostate enlargement (BPE). METHODS: The microbiota of prostate tissues and catheterized urine from 15 patients were analyzed by 16S metagenomic analysis and compared to...

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Autores principales: Okada, Koichi, Takezawa, Kentaro, Tsujimura, Go, Imanaka, Takahiro, Kuribayashi, Sohei, Ueda, Norichika, Hatano, Koji, Fukuhara, Shinichiro, Kiuchi, Hiroshi, Fujita, Kazutoshi, Motooka, Daisuke, Nakamura, Shota, Koyama, Yoshihisa, Shimada, Shoichi, Nonomura, Norio
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9768196/
https://www.ncbi.nlm.nih.gov/pubmed/36569206
http://dx.doi.org/10.3389/fcimb.2022.1048319
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author Okada, Koichi
Takezawa, Kentaro
Tsujimura, Go
Imanaka, Takahiro
Kuribayashi, Sohei
Ueda, Norichika
Hatano, Koji
Fukuhara, Shinichiro
Kiuchi, Hiroshi
Fujita, Kazutoshi
Motooka, Daisuke
Nakamura, Shota
Koyama, Yoshihisa
Shimada, Shoichi
Nonomura, Norio
author_facet Okada, Koichi
Takezawa, Kentaro
Tsujimura, Go
Imanaka, Takahiro
Kuribayashi, Sohei
Ueda, Norichika
Hatano, Koji
Fukuhara, Shinichiro
Kiuchi, Hiroshi
Fujita, Kazutoshi
Motooka, Daisuke
Nakamura, Shota
Koyama, Yoshihisa
Shimada, Shoichi
Nonomura, Norio
author_sort Okada, Koichi
collection PubMed
description INTRODUCTION: We aimed to clarify the presence and localization of the prostate microbiota and examine its association with benign prostate enlargement (BPE). METHODS: The microbiota of prostate tissues and catheterized urine from 15 patients were analyzed by 16S metagenomic analysis and compared to show that the prostate microbiota was not a contaminant of the urinary microbiota. Fluorescence in situ hybridization (FISH) and in situ hybridization (ISH) using the specific probe for eubacteria was performed on prostate tissue to show the localization of bacteria in the prostate. The BPE group was defined as prostate volume ≥30 mL, and the non-BPE group as prostate volume <30 mL. The microbiota of the two groups were compared to clarify the association between prostate microbiota and BPE. RESULTS: Faith’s phylogenetic diversity index of prostate tissue was significantly higher than that of urine (42.3±3.8 vs 25.5±5.6, P=0.01). Principal coordinate analysis showed a significant difference between the microbiota of prostate tissue and catheterized urine (P<0.01). FISH and ISH showed the presence of bacteria in the prostatic duct. Comparison of prostate microbiota between the BPE and non-BPE groups showed that the Chao1 index of the BPE group was significantly lower than that of the latter [142 (50–316) vs 169 (97–665), P=0.047] and the abundance of Burkholderia was significantly higher in the BPE group than in the latter. CONCLUSIONS: We demonstrated that the prostate microbiota was located in the prostatic duct and reduced diversity of prostate microbiota was associated with BPE, suggesting that prostate microbiota plays a role in BPE.
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spelling pubmed-97681962022-12-22 Localization and potential role of prostate microbiota Okada, Koichi Takezawa, Kentaro Tsujimura, Go Imanaka, Takahiro Kuribayashi, Sohei Ueda, Norichika Hatano, Koji Fukuhara, Shinichiro Kiuchi, Hiroshi Fujita, Kazutoshi Motooka, Daisuke Nakamura, Shota Koyama, Yoshihisa Shimada, Shoichi Nonomura, Norio Front Cell Infect Microbiol Cellular and Infection Microbiology INTRODUCTION: We aimed to clarify the presence and localization of the prostate microbiota and examine its association with benign prostate enlargement (BPE). METHODS: The microbiota of prostate tissues and catheterized urine from 15 patients were analyzed by 16S metagenomic analysis and compared to show that the prostate microbiota was not a contaminant of the urinary microbiota. Fluorescence in situ hybridization (FISH) and in situ hybridization (ISH) using the specific probe for eubacteria was performed on prostate tissue to show the localization of bacteria in the prostate. The BPE group was defined as prostate volume ≥30 mL, and the non-BPE group as prostate volume <30 mL. The microbiota of the two groups were compared to clarify the association between prostate microbiota and BPE. RESULTS: Faith’s phylogenetic diversity index of prostate tissue was significantly higher than that of urine (42.3±3.8 vs 25.5±5.6, P=0.01). Principal coordinate analysis showed a significant difference between the microbiota of prostate tissue and catheterized urine (P<0.01). FISH and ISH showed the presence of bacteria in the prostatic duct. Comparison of prostate microbiota between the BPE and non-BPE groups showed that the Chao1 index of the BPE group was significantly lower than that of the latter [142 (50–316) vs 169 (97–665), P=0.047] and the abundance of Burkholderia was significantly higher in the BPE group than in the latter. CONCLUSIONS: We demonstrated that the prostate microbiota was located in the prostatic duct and reduced diversity of prostate microbiota was associated with BPE, suggesting that prostate microbiota plays a role in BPE. Frontiers Media S.A. 2022-12-07 /pmc/articles/PMC9768196/ /pubmed/36569206 http://dx.doi.org/10.3389/fcimb.2022.1048319 Text en Copyright © 2022 Okada, Takezawa, Tsujimura, Imanaka, Kuribayashi, Ueda, Hatano, Fukuhara, Kiuchi, Fujita, Motooka, Nakamura, Koyama, Shimada and Nonomura https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Cellular and Infection Microbiology
Okada, Koichi
Takezawa, Kentaro
Tsujimura, Go
Imanaka, Takahiro
Kuribayashi, Sohei
Ueda, Norichika
Hatano, Koji
Fukuhara, Shinichiro
Kiuchi, Hiroshi
Fujita, Kazutoshi
Motooka, Daisuke
Nakamura, Shota
Koyama, Yoshihisa
Shimada, Shoichi
Nonomura, Norio
Localization and potential role of prostate microbiota
title Localization and potential role of prostate microbiota
title_full Localization and potential role of prostate microbiota
title_fullStr Localization and potential role of prostate microbiota
title_full_unstemmed Localization and potential role of prostate microbiota
title_short Localization and potential role of prostate microbiota
title_sort localization and potential role of prostate microbiota
topic Cellular and Infection Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9768196/
https://www.ncbi.nlm.nih.gov/pubmed/36569206
http://dx.doi.org/10.3389/fcimb.2022.1048319
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