Low atmospheric CO(2) levels before the rise of forested ecosystems

The emergence of forests on Earth (~385 million years ago, Ma)(1) has been linked to an order-of-magnitude decline in atmospheric CO(2) levels and global climatic cooling by altering continental weathering processes, but observational constraints on atmospheric CO(2) before the rise of forests carry large, often unbound, uncertainties. Here, we calibrate a mechanistic model for gas exchange in modern lycophytes and constrain atmospheric CO(2) levels 410–380 Ma from related fossilized plants with bound uncertainties of approximately ±100 ppm (1 sd). We find that the atmosphere contained ~525–715 ppm CO(2) before continents were afforested, and that Earth was partially glaciated according to a palaeoclimate model. A process-driven biogeochemical model (COPSE) shows the appearance of trees with deep roots did not dramatically enhance atmospheric CO(2) removal. Rather, shallow-rooted vascular ecosystems could have simultaneously caused abrupt atmospheric oxygenation and climatic cooling long before the rise of forests, although earlier CO(2) levels are still unknown.