Cargando…

Indocyanine green as a near-infrared theranostic agent for ferroptosis and apoptosis-based, photothermal, and photodynamic cancer therapy

Ferroptosis is a recently discovered programmed cell death pathway initiated by reactive oxygen species (ROS). Cancer cells can escape ferroptosis, and strategies to promote cancer treatment are crucial. Indocyanine green (ICG) is a near-infrared (NIR) fluorescent molecule used in the imaging of res...

Descripción completa

Detalles Bibliográficos
Autores principales: Tseng, Hsiang-Ching, Kuo, Chan-Yen, Liao, Wei-Ting, Chou, Te-Sen, Hsiao, Jong-Kai
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9768228/
https://www.ncbi.nlm.nih.gov/pubmed/36567945
http://dx.doi.org/10.3389/fmolb.2022.1045885
_version_ 1784854121542582272
author Tseng, Hsiang-Ching
Kuo, Chan-Yen
Liao, Wei-Ting
Chou, Te-Sen
Hsiao, Jong-Kai
author_facet Tseng, Hsiang-Ching
Kuo, Chan-Yen
Liao, Wei-Ting
Chou, Te-Sen
Hsiao, Jong-Kai
author_sort Tseng, Hsiang-Ching
collection PubMed
description Ferroptosis is a recently discovered programmed cell death pathway initiated by reactive oxygen species (ROS). Cancer cells can escape ferroptosis, and strategies to promote cancer treatment are crucial. Indocyanine green (ICG) is a near-infrared (NIR) fluorescent molecule used in the imaging of residual tumor removal during surgery. Growing attention has been paid to the anticancer potential of ICG-NIR irradiation by inducing ROS production and theranostic effects. Organic anion transmembrane polypeptide (OATP) 1B3 is responsible for ICG metabolism. Additionally, the overexpression of OATP1B3 has been reported in several cancers. However, whether ICG combined with NIR exposure can cause ferroptosis remains unknown and the concept of treating OATP1B3-expressing cells with ICG-NIR irradiation has not been validated. We then used ICG as a theranostic molecule and an OATP1B3-transfected fibrosarcoma cell line, HT-1080 (HT-1080-OATP1B3), as a cell model. The HT-1080-OATP1B3 cell could promote the uptake of ICG into the cytoplasm. We observed that the HT-1080-OATP1B3 cells treated with ICG and exposed to 808-nm laser irradiation underwent apoptosis, as indicated by a reduction in mitochondrial membrane potential, and upregulation of cleaved Caspase-3 and Bax but downregulation of Bcl-2 expression. Moreover, lipid ROS production and consequent ferroptosis and hyperthermic effect were noted after ICG and laser administration. Finally, in vivo study findings also revealed that ICG with 808-nm laser irradiation has a significant effect on cancer suppression. ICG is a theranostic molecule that exerts synchronous apoptosis, ferroptosis, and hyperthermia effects and thus can be used in cancer treatment. Our findings may facilitate the development of treatment modalities for chemo-resistant cancers.
format Online
Article
Text
id pubmed-9768228
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher Frontiers Media S.A.
record_format MEDLINE/PubMed
spelling pubmed-97682282022-12-22 Indocyanine green as a near-infrared theranostic agent for ferroptosis and apoptosis-based, photothermal, and photodynamic cancer therapy Tseng, Hsiang-Ching Kuo, Chan-Yen Liao, Wei-Ting Chou, Te-Sen Hsiao, Jong-Kai Front Mol Biosci Molecular Biosciences Ferroptosis is a recently discovered programmed cell death pathway initiated by reactive oxygen species (ROS). Cancer cells can escape ferroptosis, and strategies to promote cancer treatment are crucial. Indocyanine green (ICG) is a near-infrared (NIR) fluorescent molecule used in the imaging of residual tumor removal during surgery. Growing attention has been paid to the anticancer potential of ICG-NIR irradiation by inducing ROS production and theranostic effects. Organic anion transmembrane polypeptide (OATP) 1B3 is responsible for ICG metabolism. Additionally, the overexpression of OATP1B3 has been reported in several cancers. However, whether ICG combined with NIR exposure can cause ferroptosis remains unknown and the concept of treating OATP1B3-expressing cells with ICG-NIR irradiation has not been validated. We then used ICG as a theranostic molecule and an OATP1B3-transfected fibrosarcoma cell line, HT-1080 (HT-1080-OATP1B3), as a cell model. The HT-1080-OATP1B3 cell could promote the uptake of ICG into the cytoplasm. We observed that the HT-1080-OATP1B3 cells treated with ICG and exposed to 808-nm laser irradiation underwent apoptosis, as indicated by a reduction in mitochondrial membrane potential, and upregulation of cleaved Caspase-3 and Bax but downregulation of Bcl-2 expression. Moreover, lipid ROS production and consequent ferroptosis and hyperthermic effect were noted after ICG and laser administration. Finally, in vivo study findings also revealed that ICG with 808-nm laser irradiation has a significant effect on cancer suppression. ICG is a theranostic molecule that exerts synchronous apoptosis, ferroptosis, and hyperthermia effects and thus can be used in cancer treatment. Our findings may facilitate the development of treatment modalities for chemo-resistant cancers. Frontiers Media S.A. 2022-12-07 /pmc/articles/PMC9768228/ /pubmed/36567945 http://dx.doi.org/10.3389/fmolb.2022.1045885 Text en Copyright © 2022 Tseng, Kuo, Liao, Chou and Hsiao. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Molecular Biosciences
Tseng, Hsiang-Ching
Kuo, Chan-Yen
Liao, Wei-Ting
Chou, Te-Sen
Hsiao, Jong-Kai
Indocyanine green as a near-infrared theranostic agent for ferroptosis and apoptosis-based, photothermal, and photodynamic cancer therapy
title Indocyanine green as a near-infrared theranostic agent for ferroptosis and apoptosis-based, photothermal, and photodynamic cancer therapy
title_full Indocyanine green as a near-infrared theranostic agent for ferroptosis and apoptosis-based, photothermal, and photodynamic cancer therapy
title_fullStr Indocyanine green as a near-infrared theranostic agent for ferroptosis and apoptosis-based, photothermal, and photodynamic cancer therapy
title_full_unstemmed Indocyanine green as a near-infrared theranostic agent for ferroptosis and apoptosis-based, photothermal, and photodynamic cancer therapy
title_short Indocyanine green as a near-infrared theranostic agent for ferroptosis and apoptosis-based, photothermal, and photodynamic cancer therapy
title_sort indocyanine green as a near-infrared theranostic agent for ferroptosis and apoptosis-based, photothermal, and photodynamic cancer therapy
topic Molecular Biosciences
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9768228/
https://www.ncbi.nlm.nih.gov/pubmed/36567945
http://dx.doi.org/10.3389/fmolb.2022.1045885
work_keys_str_mv AT tsenghsiangching indocyaninegreenasanearinfraredtheranosticagentforferroptosisandapoptosisbasedphotothermalandphotodynamiccancertherapy
AT kuochanyen indocyaninegreenasanearinfraredtheranosticagentforferroptosisandapoptosisbasedphotothermalandphotodynamiccancertherapy
AT liaoweiting indocyaninegreenasanearinfraredtheranosticagentforferroptosisandapoptosisbasedphotothermalandphotodynamiccancertherapy
AT choutesen indocyaninegreenasanearinfraredtheranosticagentforferroptosisandapoptosisbasedphotothermalandphotodynamiccancertherapy
AT hsiaojongkai indocyaninegreenasanearinfraredtheranosticagentforferroptosisandapoptosisbasedphotothermalandphotodynamiccancertherapy