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Novel insights into mouse models of ectopic proplatelet release
Mature bone marrow (BM) megakaryocytes (MKs) produce platelets by extending proplatelets into sinusoidal blood vessels. Defects in this process can lead to thrombocytopenia and increased risk of bleeding. Mice lacking the actin-regulatory proteins Profilin 1 (PFN1), Wiskott–Aldrich Syndrome protein...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
The American Society of Hematology
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9768245/ https://www.ncbi.nlm.nih.gov/pubmed/36251748 http://dx.doi.org/10.1182/bloodadvances.2022007824 |
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author | Spindler, Markus Bergmeier, Wolfgang Stradal, Theresia E. B. Zhang, Jinyi Siminovitch, Katherine A. Nicolai, Leo Reinhold, Annegret Bender, Markus |
author_facet | Spindler, Markus Bergmeier, Wolfgang Stradal, Theresia E. B. Zhang, Jinyi Siminovitch, Katherine A. Nicolai, Leo Reinhold, Annegret Bender, Markus |
author_sort | Spindler, Markus |
collection | PubMed |
description | Mature bone marrow (BM) megakaryocytes (MKs) produce platelets by extending proplatelets into sinusoidal blood vessels. Defects in this process can lead to thrombocytopenia and increased risk of bleeding. Mice lacking the actin-regulatory proteins Profilin 1 (PFN1), Wiskott–Aldrich Syndrome protein (WASp), Actin Related Protein 2/3 complex (Arp2/3), or adhesion and degranulation-promoting adapter protein (ADAP) display thrombocytopenia and ectopic release of (pro)platelet-like particles into the BM compartment, pointing to an important axis of actin-mediated directional proplatelet formation. The mechanism underlying ectopic release in these mice is still not completely understood. However, we hypothesized that similar functional defects account for this observation. We analyzed WASp-, ADAP-, PFN1-, and ARPC2-knockout mice to determine the role of actin reorganization and integrin activation in directional proplatelet formation. ADAP-, ARPC2-, and PFN1-deficient MKs displayed reduced adhesion to collagen, defective F-actin organization, and diminished β1-integrin activation. WASp-deficient MKs showed the strongest reduction in the adhesion assay of collagen and altered F-actin organization with reduced podosome formation. Our results indicate that ADAP, PFN1, WASp, and ARP2/3 are part of the same pathway that regulates polarization processes in MKs and directional proplatelet formation into BM sinusoids. |
format | Online Article Text |
id | pubmed-9768245 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | The American Society of Hematology |
record_format | MEDLINE/PubMed |
spelling | pubmed-97682452022-12-28 Novel insights into mouse models of ectopic proplatelet release Spindler, Markus Bergmeier, Wolfgang Stradal, Theresia E. B. Zhang, Jinyi Siminovitch, Katherine A. Nicolai, Leo Reinhold, Annegret Bender, Markus Blood Adv Stimulus Report Mature bone marrow (BM) megakaryocytes (MKs) produce platelets by extending proplatelets into sinusoidal blood vessels. Defects in this process can lead to thrombocytopenia and increased risk of bleeding. Mice lacking the actin-regulatory proteins Profilin 1 (PFN1), Wiskott–Aldrich Syndrome protein (WASp), Actin Related Protein 2/3 complex (Arp2/3), or adhesion and degranulation-promoting adapter protein (ADAP) display thrombocytopenia and ectopic release of (pro)platelet-like particles into the BM compartment, pointing to an important axis of actin-mediated directional proplatelet formation. The mechanism underlying ectopic release in these mice is still not completely understood. However, we hypothesized that similar functional defects account for this observation. We analyzed WASp-, ADAP-, PFN1-, and ARPC2-knockout mice to determine the role of actin reorganization and integrin activation in directional proplatelet formation. ADAP-, ARPC2-, and PFN1-deficient MKs displayed reduced adhesion to collagen, defective F-actin organization, and diminished β1-integrin activation. WASp-deficient MKs showed the strongest reduction in the adhesion assay of collagen and altered F-actin organization with reduced podosome formation. Our results indicate that ADAP, PFN1, WASp, and ARP2/3 are part of the same pathway that regulates polarization processes in MKs and directional proplatelet formation into BM sinusoids. The American Society of Hematology 2022-10-20 /pmc/articles/PMC9768245/ /pubmed/36251748 http://dx.doi.org/10.1182/bloodadvances.2022007824 Text en © 2022 by The American Society of Hematology. Licensed under Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0), permitting only noncommercial, nonderivative use with attribution. All other rights reserved. https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). |
spellingShingle | Stimulus Report Spindler, Markus Bergmeier, Wolfgang Stradal, Theresia E. B. Zhang, Jinyi Siminovitch, Katherine A. Nicolai, Leo Reinhold, Annegret Bender, Markus Novel insights into mouse models of ectopic proplatelet release |
title | Novel insights into mouse models of ectopic proplatelet release |
title_full | Novel insights into mouse models of ectopic proplatelet release |
title_fullStr | Novel insights into mouse models of ectopic proplatelet release |
title_full_unstemmed | Novel insights into mouse models of ectopic proplatelet release |
title_short | Novel insights into mouse models of ectopic proplatelet release |
title_sort | novel insights into mouse models of ectopic proplatelet release |
topic | Stimulus Report |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9768245/ https://www.ncbi.nlm.nih.gov/pubmed/36251748 http://dx.doi.org/10.1182/bloodadvances.2022007824 |
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