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Neuroprotective effects of the fractions of Ocimum basilicum in seizures induced by pentylenetetrazole in mice

OBJECTIVE: Neuroprotective and antioxidant effects of Ocimum basilicum (O. basilicum) against pentylenetetrazole (PTZ)-induced seizures were investigated. MATERIALS AND METHODS: Mice were divided as follows: (Group 1) Control, (Group 2) PTZ, (Groups 3-5) 50,100 and 200 mg/kg hydro-ethanolic (HE) ext...

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Autores principales: Mansouri, Somaieh, Hosseini, Mahmoud, Alipour, Fatemeh, Beheshti, Farimah, Rakhshandeh, Hassan, Mohammadipour, Abbas, Anaeigoudari, Akbar, Jalili-nik, Mohammad, Khazdair, Mohammad Reza, Jahani, Amirali
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Mashhad University of Medical Sciences 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9768854/
https://www.ncbi.nlm.nih.gov/pubmed/36583175
http://dx.doi.org/10.22038/AJP.2022.20470
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author Mansouri, Somaieh
Hosseini, Mahmoud
Alipour, Fatemeh
Beheshti, Farimah
Rakhshandeh, Hassan
Mohammadipour, Abbas
Anaeigoudari, Akbar
Jalili-nik, Mohammad
Khazdair, Mohammad Reza
Jahani, Amirali
author_facet Mansouri, Somaieh
Hosseini, Mahmoud
Alipour, Fatemeh
Beheshti, Farimah
Rakhshandeh, Hassan
Mohammadipour, Abbas
Anaeigoudari, Akbar
Jalili-nik, Mohammad
Khazdair, Mohammad Reza
Jahani, Amirali
author_sort Mansouri, Somaieh
collection PubMed
description OBJECTIVE: Neuroprotective and antioxidant effects of Ocimum basilicum (O. basilicum) against pentylenetetrazole (PTZ)-induced seizures were investigated. MATERIALS AND METHODS: Mice were divided as follows: (Group 1) Control, (Group 2) PTZ, (Groups 3-5) 50,100 and 200 mg/kg hydro-ethanolic (HE) extract, and (Groups 6-8) 200 mg/kg ethyl-acetate (EAF), N-hexane (NHF) and water (WF) fractions. Minimal clonic seizures (MCS) and generalized tonic-clonic seizures (GTCS) latencies were measured. Biochemical and histological studies were done. RESULTS: MCS and GTCS latency in HE groups were longer than the PTZ group (p<0.05 to p<0.001). EAF and NHF prolonged the onset of MCS and GTCS (p<0.001). PTZ increased malondialdehyde (MDA) and dark neuron (DN) production while decreased thiol, catalase (CAT) and superoxide dismutase (SOD) (p<0.05 to p<0.001). Pre-treatment by HE and all fractions of the plant attenuated MDA and DN while increased thiol, CAT and SOD (p<0.01 to p<0.001). CONCLUSION: EAF and NHF had anticonvulsant properties. The extract and fractions protected the brain from PTZ-induced oxidative damages and showed neuroprotective effects.
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spelling pubmed-97688542022-12-28 Neuroprotective effects of the fractions of Ocimum basilicum in seizures induced by pentylenetetrazole in mice Mansouri, Somaieh Hosseini, Mahmoud Alipour, Fatemeh Beheshti, Farimah Rakhshandeh, Hassan Mohammadipour, Abbas Anaeigoudari, Akbar Jalili-nik, Mohammad Khazdair, Mohammad Reza Jahani, Amirali Avicenna J Phytomed Original Research Article OBJECTIVE: Neuroprotective and antioxidant effects of Ocimum basilicum (O. basilicum) against pentylenetetrazole (PTZ)-induced seizures were investigated. MATERIALS AND METHODS: Mice were divided as follows: (Group 1) Control, (Group 2) PTZ, (Groups 3-5) 50,100 and 200 mg/kg hydro-ethanolic (HE) extract, and (Groups 6-8) 200 mg/kg ethyl-acetate (EAF), N-hexane (NHF) and water (WF) fractions. Minimal clonic seizures (MCS) and generalized tonic-clonic seizures (GTCS) latencies were measured. Biochemical and histological studies were done. RESULTS: MCS and GTCS latency in HE groups were longer than the PTZ group (p<0.05 to p<0.001). EAF and NHF prolonged the onset of MCS and GTCS (p<0.001). PTZ increased malondialdehyde (MDA) and dark neuron (DN) production while decreased thiol, catalase (CAT) and superoxide dismutase (SOD) (p<0.05 to p<0.001). Pre-treatment by HE and all fractions of the plant attenuated MDA and DN while increased thiol, CAT and SOD (p<0.01 to p<0.001). CONCLUSION: EAF and NHF had anticonvulsant properties. The extract and fractions protected the brain from PTZ-induced oxidative damages and showed neuroprotective effects. Mashhad University of Medical Sciences 2022 /pmc/articles/PMC9768854/ /pubmed/36583175 http://dx.doi.org/10.22038/AJP.2022.20470 Text en https://creativecommons.org/licenses/by/3.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution License, (http://creativecommons.org/licenses/by/3.0/ (https://creativecommons.org/licenses/by/3.0/) ) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Research Article
Mansouri, Somaieh
Hosseini, Mahmoud
Alipour, Fatemeh
Beheshti, Farimah
Rakhshandeh, Hassan
Mohammadipour, Abbas
Anaeigoudari, Akbar
Jalili-nik, Mohammad
Khazdair, Mohammad Reza
Jahani, Amirali
Neuroprotective effects of the fractions of Ocimum basilicum in seizures induced by pentylenetetrazole in mice
title Neuroprotective effects of the fractions of Ocimum basilicum in seizures induced by pentylenetetrazole in mice
title_full Neuroprotective effects of the fractions of Ocimum basilicum in seizures induced by pentylenetetrazole in mice
title_fullStr Neuroprotective effects of the fractions of Ocimum basilicum in seizures induced by pentylenetetrazole in mice
title_full_unstemmed Neuroprotective effects of the fractions of Ocimum basilicum in seizures induced by pentylenetetrazole in mice
title_short Neuroprotective effects of the fractions of Ocimum basilicum in seizures induced by pentylenetetrazole in mice
title_sort neuroprotective effects of the fractions of ocimum basilicum in seizures induced by pentylenetetrazole in mice
topic Original Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9768854/
https://www.ncbi.nlm.nih.gov/pubmed/36583175
http://dx.doi.org/10.22038/AJP.2022.20470
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