Cargando…

Usefulness of Combining Sputum and Nasopharyngeal Samples for Viral Detection by Reverse Transcriptase PCR in Adults Hospitalized with Acute Respiratory Illness

Nasopharyngeal swabs (NPS) or washings have traditionally been used to diagnose respiratory tract infections. Reverse transcriptase PCR (RT-PCR) is widely used for rapid viral detection using samples from the upper respiratory tract. However, RT-PCR is rarely applied to sputum samples, mainly due to...

Descripción completa

Detalles Bibliográficos
Autores principales:	Kim, Keun Ju, Yun, Seung Gyu, Cho, Yunjung, Nam, Myung-Hyun, Lee, Chang Kyu
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	American Society for Microbiology 2022
Materias:	Research Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9769557/ https://www.ncbi.nlm.nih.gov/pubmed/36374089 http://dx.doi.org/10.1128/spectrum.02775-22

Ejemplares similares

371. Adding sputum and saliva to nasopharyngeal swab samples for PCR detection of Respiratory Syncytial Virus in adults hospitalized with acute respiratory illness may double case detection
por: Ramirez, Julio A, et al.
Publicado: (2022)

Sputum versus nasopharyngeal samples for the molecular diagnosis of respiratory viral infection in cystic fibrosis: A pilot study
por: Cardot-Martin, Emilie, et al.
Publicado: (2021)

Application of Reverse Transcriptase –PCR (RT-PCR) for rapid detection of viable Escherichia coli in drinking water samples
por: Molaee, Neda, et al.
Publicado: (2015)

lytA Quantitative PCR on Sputum and Nasopharyngeal Swab Samples for Detection of Pneumococcal Pneumonia among the Elderly
por: Saukkoriipi, Annika, et al.
Publicado: (2017)

Validation of a multiplex reverse transcriptase PCR ELISA for the detection of 19 respiratory tract pathogens
por: Puppe, W., et al.
Publicado: (2012)

Reverse transcriptase droplet digital PCR shows high resilience to PCR inhibitors from plant, soil and water samples
por: Rački, Nejc, et al.
Publicado: (2014)

PCR inhibition by reverse transcriptase leads to an overestimation of amplification efficiency
por: Suslov, Oleg, et al.
Publicado: (2005)

Association between lipid profiles and viral respiratory infections in human sputum samples
por: Humes, Sara T., et al.
Publicado: (2022)

AUSTRALIA ANTIGEN, CORONAVIRUS, AND REVERSE TRANSCRIPTASE IN VIRAL HEPATITIS
por: Sirtori, Carlo
Publicado: (1971)

Viral Load Dynamics in Sputum and Nasopharyngeal Swab in Patients with COVID-19
por: Liu, R., et al.
Publicado: (2020)

Evaluation of the AdvanSure™ real-time RT-PCR compared with culture and Seeplex RV15 for simultaneous detection of respiratory viruses()()()
por: Cho, Chi Hyun, et al.
Publicado: (2014)

COVID reverse transcriptase PCR in private laboratories: From theory to reality
por: Autissier, M., et al.
Publicado: (2020)

Structures of Substrate Complexes of Foamy Viral Protease-Reverse Transcriptase
por: Nowacka, Marzena, et al.
Publicado: (2021)

Panspecies molecular assays detect viral pathogens missed by real-time PCR/reverse-transcriptase PCR among pneumonia patients, Sarawak, Malaysia
por: Fieldhouse, Jane K., et al.
Publicado: (2020)

Real-Time Quantitative Fluorescent Reverse Transcriptase-PCR for Detection of Severe Acute Respiratory Syndrome-Associated Coronavirus RNA
por: Chen, Weijun, et al.
Publicado: (2012)

Comparison of electronic microarray to enzyme hybridization assay for multiplex reverse-transcriptase PCR detection of common respiratory viruses in children
por: Henrickson, K.J., et al.
Publicado: (2007)

A Reverse Transcriptase-PCR Assay for Detecting Filarial Infective Larvae in Mosquitoes
por: Laney, Sandra J., et al.
Publicado: (2008)

Diagnosis of Respiratory Syncytial Virus in Adults Substantially Increases When Adding Sputum, Saliva, and Serology Testing to Nasopharyngeal Swab RT–PCR
por: Ramirez, Julio, et al.
Publicado: (2023)

Comparison of three multiplex PCR assays for detection of respiratory viruses: Anyplex II RV16, AdvanSure RV, and Real‐Q RV
por: Yun, Seung Gyu, et al.
Publicado: (2017)

Evaluation of a novel real-time RT-PCR using TOCE technology compared with culture and Seeplex RV15 for simultaneous detection of respiratory viruses
por: Cho, Chi Hyun, et al.
Publicado: (2013)

Nuclear translocation of telomerase reverse transcriptase is a critical process in lymphatic metastasis of nasopharyngeal carcinoma
por: WU, TING-TING, et al.
Publicado: (2015)

Diagnostic Accuracy of the Panbio Severe Acute Respiratory Syndrome Coronavirus 2 Antigen Rapid Test Compared with Reverse-Transcriptase Polymerase Chain Reaction Testing of Nasopharyngeal Samples in the Pediatric Population
por: Villaverde, Serena, et al.
Publicado: (2021)

Comparison of nasal swabs with throat swabs for the detection of respiratory viruses by real-time reverse transcriptase PCR in adult Hajj pilgrims
por: Benkouiten, Samir, et al.
Publicado: (2015)

Validation of a Methodology for the Detection of Severe Acute Respiratory Syndrome Coronavirus 2 in Saliva by Real-Time Reverse Transcriptase-PCR
por: Escobar, Daniel F., et al.
Publicado: (2021)

Alternative RNA extraction-free techniques for the real-time RT-PCR detection of SARS-CoV-2 in nasopharyngeal swab and sputum samples
por: Villota, Stephany D., et al.
Publicado: (2021)

Viral etiology of respiratory infections in children in southwestern Saudi Arabia using multiplex reverse-transcriptase polymerase chain reaction
por: Al-Ayed, Mohamed S., et al.
Publicado: (2014)

Significance of viral infections detected by reverse-transcriptase - multiplex PCR on hospital-related outcomes in acute exacerbations of chronic obstructive pulmonary disease
por: Mohan, Anant, et al.
Publicado: (2015)

Development and Evaluation of an Enterovirus D68 Real-Time Reverse Transcriptase PCR Assay
por: Wylie, Todd N., et al.
Publicado: (2015)

Hot start reverse transcriptase: an approach for improved real-time RT-PCR performance
por: Rutschke, Nils, et al.
Publicado: (2015)

Comparative Evaluation of Three Homogenization Methods for Isolating Middle East Respiratory Syndrome Coronavirus Nucleic Acids From Sputum Samples for Real-Time Reverse Transcription PCR
por: Sung, Heungsup, et al.
Publicado: (2016)

A reverse transcriptase ribozyme
por: Samanta, Biswajit, et al.
Publicado: (2017)

Heminested reverse-transcriptase polymerase chain reaction (hnRT-PCR) as a tool for rabies virus detection in stored and decomposed samples
por: Araújo, Danielle B, et al.
Publicado: (2008)

Reverse transcriptase activity in psoriatic lesions: effects of reverse transcriptase inhibitors on keratinocyte proliferation and differentiation
por: Molès, Jean-Pierre, et al.
Publicado: (2009)

Reverse-Transcriptase PCR Detection of Leptospira: Absence of Agreement with Single-Specimen Microscopic Agglutination Testing
por: Waggoner, Jesse J., et al.
Publicado: (2015)

Low rates of nucleoside reverse transcriptase inhibitor and nonnucleoside reverse transcriptase inhibitor drug resistance in Botswana
por: Moyo, Sikhulile, et al.
Publicado: (2019)

Appraisal of a Simple and Effective RT-qPCR Assay for Evaluating the Reverse Transcriptase Activity in Blood Samples from HIV-1 Patients
por: Macchi, Beatrice, et al.
Publicado: (2020)

Kinetic variations between reverse transcriptases of viral protein X coding and noncoding lentiviruses
por: Lenzi, Gina M, et al.
Publicado: (2014)

Reverse transcriptase-free detection of viral RNA using Hemo Klentaq DNA polymerase
por: Sakhabutdinova, Assol R., et al.
Publicado: (2022)

Rapid detection of a highly virulent Chinese-type isolate of Porcine Reproductive and Respiratory Syndrome virus by real-time reverse transcriptase PCR
por: Xiao, Xing-Long, et al.
Publicado: (2008)

Telomerase reverse transcriptase and neurodegenerative diseases
por: Yu, Xin, et al.
Publicado: (2023)

Cannot write session to /tmp/vufind_sessions/sess_qqs2gh8h4stohhidbhs26915qp