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Associations of Rap1 with Cell Wall Integrity, Biofilm Formation, and Virulence in Candida albicans
Rap1 (repressor activator protein 1) is a multifunctional protein, playing important roles in telomeric and nontelomeric functions in many eukaryotes. Candida albicans Rap1 has been previously shown to be involved in telomeric regulation, but its other functions are still mostly unknown. In this stu...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
American Society for Microbiology
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9769648/ https://www.ncbi.nlm.nih.gov/pubmed/36416583 http://dx.doi.org/10.1128/spectrum.03285-22 |
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author | Wang, Wen-Han Lai, Ting-Xiu Wu, Yi-Chia Chen, Zzu-Ting Tseng, Kuo-Yun Lan, Chung-Yu |
author_facet | Wang, Wen-Han Lai, Ting-Xiu Wu, Yi-Chia Chen, Zzu-Ting Tseng, Kuo-Yun Lan, Chung-Yu |
author_sort | Wang, Wen-Han |
collection | PubMed |
description | Rap1 (repressor activator protein 1) is a multifunctional protein, playing important roles in telomeric and nontelomeric functions in many eukaryotes. Candida albicans Rap1 has been previously shown to be involved in telomeric regulation, but its other functions are still mostly unknown. In this study, we found that the deletion of the RAP1 gene altered cell wall properties, composition, and gene expression. In addition, deletion of RAP1 affected C. albicans biofilm formation and modulated phagocytosis and cytokine release by host immune cells. Finally, the RAP1 gene deletion mutant showed attenuation of C. albicans virulence in a Galleria mellonella infection model. Therefore, these findings provide new insights into Rap1 functions that are particularly relevant to pathogenesis and virulence of C. albicans. IMPORTANCE C. albicans is an important fungal pathogen of humans. The cell wall is the outermost layer of C. albicans and is important for commensalism and infection by this pathogen. Moreover, the cell wall is also an important target for antifungals. Studies of how C. albicans maintains its cell wall integrity are critical for a better understanding of fungal pathogenesis and virulence. This work focuses on exploring unknown functions of C. albicans Rap1 and reveals its contribution to cell wall integrity, biofilm formation, and virulence. Notably, these findings will also improve our general understanding of complex machinery to control pathogenesis and virulence of fungal pathogens. |
format | Online Article Text |
id | pubmed-9769648 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | American Society for Microbiology |
record_format | MEDLINE/PubMed |
spelling | pubmed-97696482022-12-22 Associations of Rap1 with Cell Wall Integrity, Biofilm Formation, and Virulence in Candida albicans Wang, Wen-Han Lai, Ting-Xiu Wu, Yi-Chia Chen, Zzu-Ting Tseng, Kuo-Yun Lan, Chung-Yu Microbiol Spectr Research Article Rap1 (repressor activator protein 1) is a multifunctional protein, playing important roles in telomeric and nontelomeric functions in many eukaryotes. Candida albicans Rap1 has been previously shown to be involved in telomeric regulation, but its other functions are still mostly unknown. In this study, we found that the deletion of the RAP1 gene altered cell wall properties, composition, and gene expression. In addition, deletion of RAP1 affected C. albicans biofilm formation and modulated phagocytosis and cytokine release by host immune cells. Finally, the RAP1 gene deletion mutant showed attenuation of C. albicans virulence in a Galleria mellonella infection model. Therefore, these findings provide new insights into Rap1 functions that are particularly relevant to pathogenesis and virulence of C. albicans. IMPORTANCE C. albicans is an important fungal pathogen of humans. The cell wall is the outermost layer of C. albicans and is important for commensalism and infection by this pathogen. Moreover, the cell wall is also an important target for antifungals. Studies of how C. albicans maintains its cell wall integrity are critical for a better understanding of fungal pathogenesis and virulence. This work focuses on exploring unknown functions of C. albicans Rap1 and reveals its contribution to cell wall integrity, biofilm formation, and virulence. Notably, these findings will also improve our general understanding of complex machinery to control pathogenesis and virulence of fungal pathogens. American Society for Microbiology 2022-11-23 /pmc/articles/PMC9769648/ /pubmed/36416583 http://dx.doi.org/10.1128/spectrum.03285-22 Text en Copyright © 2022 Wang et al. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license (https://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Research Article Wang, Wen-Han Lai, Ting-Xiu Wu, Yi-Chia Chen, Zzu-Ting Tseng, Kuo-Yun Lan, Chung-Yu Associations of Rap1 with Cell Wall Integrity, Biofilm Formation, and Virulence in Candida albicans |
title | Associations of Rap1 with Cell Wall Integrity, Biofilm Formation, and Virulence in Candida albicans |
title_full | Associations of Rap1 with Cell Wall Integrity, Biofilm Formation, and Virulence in Candida albicans |
title_fullStr | Associations of Rap1 with Cell Wall Integrity, Biofilm Formation, and Virulence in Candida albicans |
title_full_unstemmed | Associations of Rap1 with Cell Wall Integrity, Biofilm Formation, and Virulence in Candida albicans |
title_short | Associations of Rap1 with Cell Wall Integrity, Biofilm Formation, and Virulence in Candida albicans |
title_sort | associations of rap1 with cell wall integrity, biofilm formation, and virulence in candida albicans |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9769648/ https://www.ncbi.nlm.nih.gov/pubmed/36416583 http://dx.doi.org/10.1128/spectrum.03285-22 |
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