Cargando…
A Catalog of over 5,000 Metagenome-Assembled Microbial Genomes from the Caprinae Gut Microbiota
Most microbiome studies regarding the ruminant digestive tract have focused on the rumen microbiota, whereas only a few studies were performed on investigating the gut microbiota of ruminants, which limits our understanding of this important component. Herein, the gut microbiota of 30 Caprinae anima...
Autores principales: | , , , , , , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
American Society for Microbiology
2022
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9769736/ https://www.ncbi.nlm.nih.gov/pubmed/36321901 http://dx.doi.org/10.1128/spectrum.02211-22 |
_version_ | 1784854436253794304 |
---|---|
author | Zhang, Xiao-Xuan Lv, Qing-Bo Yan, Qiu-Long Zhang, Yue Guo, Ruo-Chun Meng, Jin-Xin Ma, He Qin, Si-Yuan Zhu, Qing-He Li, Chun-Qiu Liu, Rui Liu, Gang Li, Sheng-Hui Sun, Dong-Bo Ni, Hong-Bo |
author_facet | Zhang, Xiao-Xuan Lv, Qing-Bo Yan, Qiu-Long Zhang, Yue Guo, Ruo-Chun Meng, Jin-Xin Ma, He Qin, Si-Yuan Zhu, Qing-He Li, Chun-Qiu Liu, Rui Liu, Gang Li, Sheng-Hui Sun, Dong-Bo Ni, Hong-Bo |
author_sort | Zhang, Xiao-Xuan |
collection | PubMed |
description | Most microbiome studies regarding the ruminant digestive tract have focused on the rumen microbiota, whereas only a few studies were performed on investigating the gut microbiota of ruminants, which limits our understanding of this important component. Herein, the gut microbiota of 30 Caprinae animals (sheep and goats) from six provinces in China was characterized using ultradeep (>100 Gbp per sample) metagenome shotgun sequencing. An inventory of Caprinae gut microbial species containing 5,046 metagenomic assembly genomes (MAGs) was constructed. Particularly, 2,530 of the genomes belonged to uncultured candidate species. These genomes largely expanded the genomic repository of the current microbes in the Caprinae gut. Several enzymes and biosynthetic gene clusters encoded by these Caprinae gut species were identified. In summary, our study extends the gut microbiota characteristics of Caprinae and provides a basis for future studies on animal production and animal health. IMPORTANCE We constructed a microbiota catalog containing 5,046 MAGs from Caprinae gut from six regions of China. Most of the MAGs do not overlap known databases and appear to be potentially new species. We also characterized the functional spectrum of these MAGs and analyzed the differences between different regions. Our study enriches the understanding of taxonomic, functional, and metabolic diversity of Caprinae gut microbiota. We are confident that the manuscript will be of utmost interest to a wide range of readers and be widely applied in future research. |
format | Online Article Text |
id | pubmed-9769736 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | American Society for Microbiology |
record_format | MEDLINE/PubMed |
spelling | pubmed-97697362022-12-22 A Catalog of over 5,000 Metagenome-Assembled Microbial Genomes from the Caprinae Gut Microbiota Zhang, Xiao-Xuan Lv, Qing-Bo Yan, Qiu-Long Zhang, Yue Guo, Ruo-Chun Meng, Jin-Xin Ma, He Qin, Si-Yuan Zhu, Qing-He Li, Chun-Qiu Liu, Rui Liu, Gang Li, Sheng-Hui Sun, Dong-Bo Ni, Hong-Bo Microbiol Spectr Research Article Most microbiome studies regarding the ruminant digestive tract have focused on the rumen microbiota, whereas only a few studies were performed on investigating the gut microbiota of ruminants, which limits our understanding of this important component. Herein, the gut microbiota of 30 Caprinae animals (sheep and goats) from six provinces in China was characterized using ultradeep (>100 Gbp per sample) metagenome shotgun sequencing. An inventory of Caprinae gut microbial species containing 5,046 metagenomic assembly genomes (MAGs) was constructed. Particularly, 2,530 of the genomes belonged to uncultured candidate species. These genomes largely expanded the genomic repository of the current microbes in the Caprinae gut. Several enzymes and biosynthetic gene clusters encoded by these Caprinae gut species were identified. In summary, our study extends the gut microbiota characteristics of Caprinae and provides a basis for future studies on animal production and animal health. IMPORTANCE We constructed a microbiota catalog containing 5,046 MAGs from Caprinae gut from six regions of China. Most of the MAGs do not overlap known databases and appear to be potentially new species. We also characterized the functional spectrum of these MAGs and analyzed the differences between different regions. Our study enriches the understanding of taxonomic, functional, and metabolic diversity of Caprinae gut microbiota. We are confident that the manuscript will be of utmost interest to a wide range of readers and be widely applied in future research. American Society for Microbiology 2022-11-02 /pmc/articles/PMC9769736/ /pubmed/36321901 http://dx.doi.org/10.1128/spectrum.02211-22 Text en Copyright © 2022 Zhang et al. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license (https://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Research Article Zhang, Xiao-Xuan Lv, Qing-Bo Yan, Qiu-Long Zhang, Yue Guo, Ruo-Chun Meng, Jin-Xin Ma, He Qin, Si-Yuan Zhu, Qing-He Li, Chun-Qiu Liu, Rui Liu, Gang Li, Sheng-Hui Sun, Dong-Bo Ni, Hong-Bo A Catalog of over 5,000 Metagenome-Assembled Microbial Genomes from the Caprinae Gut Microbiota |
title | A Catalog of over 5,000 Metagenome-Assembled Microbial Genomes from the Caprinae Gut Microbiota |
title_full | A Catalog of over 5,000 Metagenome-Assembled Microbial Genomes from the Caprinae Gut Microbiota |
title_fullStr | A Catalog of over 5,000 Metagenome-Assembled Microbial Genomes from the Caprinae Gut Microbiota |
title_full_unstemmed | A Catalog of over 5,000 Metagenome-Assembled Microbial Genomes from the Caprinae Gut Microbiota |
title_short | A Catalog of over 5,000 Metagenome-Assembled Microbial Genomes from the Caprinae Gut Microbiota |
title_sort | catalog of over 5,000 metagenome-assembled microbial genomes from the caprinae gut microbiota |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9769736/ https://www.ncbi.nlm.nih.gov/pubmed/36321901 http://dx.doi.org/10.1128/spectrum.02211-22 |
work_keys_str_mv | AT zhangxiaoxuan acatalogofover5000metagenomeassembledmicrobialgenomesfromthecaprinaegutmicrobiota AT lvqingbo acatalogofover5000metagenomeassembledmicrobialgenomesfromthecaprinaegutmicrobiota AT yanqiulong acatalogofover5000metagenomeassembledmicrobialgenomesfromthecaprinaegutmicrobiota AT zhangyue acatalogofover5000metagenomeassembledmicrobialgenomesfromthecaprinaegutmicrobiota AT guoruochun acatalogofover5000metagenomeassembledmicrobialgenomesfromthecaprinaegutmicrobiota AT mengjinxin acatalogofover5000metagenomeassembledmicrobialgenomesfromthecaprinaegutmicrobiota AT mahe acatalogofover5000metagenomeassembledmicrobialgenomesfromthecaprinaegutmicrobiota AT qinsiyuan acatalogofover5000metagenomeassembledmicrobialgenomesfromthecaprinaegutmicrobiota AT zhuqinghe acatalogofover5000metagenomeassembledmicrobialgenomesfromthecaprinaegutmicrobiota AT lichunqiu acatalogofover5000metagenomeassembledmicrobialgenomesfromthecaprinaegutmicrobiota AT liurui acatalogofover5000metagenomeassembledmicrobialgenomesfromthecaprinaegutmicrobiota AT liugang acatalogofover5000metagenomeassembledmicrobialgenomesfromthecaprinaegutmicrobiota AT lishenghui acatalogofover5000metagenomeassembledmicrobialgenomesfromthecaprinaegutmicrobiota AT sundongbo acatalogofover5000metagenomeassembledmicrobialgenomesfromthecaprinaegutmicrobiota AT nihongbo acatalogofover5000metagenomeassembledmicrobialgenomesfromthecaprinaegutmicrobiota AT zhangxiaoxuan catalogofover5000metagenomeassembledmicrobialgenomesfromthecaprinaegutmicrobiota AT lvqingbo catalogofover5000metagenomeassembledmicrobialgenomesfromthecaprinaegutmicrobiota AT yanqiulong catalogofover5000metagenomeassembledmicrobialgenomesfromthecaprinaegutmicrobiota AT zhangyue catalogofover5000metagenomeassembledmicrobialgenomesfromthecaprinaegutmicrobiota AT guoruochun catalogofover5000metagenomeassembledmicrobialgenomesfromthecaprinaegutmicrobiota AT mengjinxin catalogofover5000metagenomeassembledmicrobialgenomesfromthecaprinaegutmicrobiota AT mahe catalogofover5000metagenomeassembledmicrobialgenomesfromthecaprinaegutmicrobiota AT qinsiyuan catalogofover5000metagenomeassembledmicrobialgenomesfromthecaprinaegutmicrobiota AT zhuqinghe catalogofover5000metagenomeassembledmicrobialgenomesfromthecaprinaegutmicrobiota AT lichunqiu catalogofover5000metagenomeassembledmicrobialgenomesfromthecaprinaegutmicrobiota AT liurui catalogofover5000metagenomeassembledmicrobialgenomesfromthecaprinaegutmicrobiota AT liugang catalogofover5000metagenomeassembledmicrobialgenomesfromthecaprinaegutmicrobiota AT lishenghui catalogofover5000metagenomeassembledmicrobialgenomesfromthecaprinaegutmicrobiota AT sundongbo catalogofover5000metagenomeassembledmicrobialgenomesfromthecaprinaegutmicrobiota AT nihongbo catalogofover5000metagenomeassembledmicrobialgenomesfromthecaprinaegutmicrobiota |