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A Catalog of over 5,000 Metagenome-Assembled Microbial Genomes from the Caprinae Gut Microbiota

Most microbiome studies regarding the ruminant digestive tract have focused on the rumen microbiota, whereas only a few studies were performed on investigating the gut microbiota of ruminants, which limits our understanding of this important component. Herein, the gut microbiota of 30 Caprinae anima...

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Detalles Bibliográficos
Autores principales: Zhang, Xiao-Xuan, Lv, Qing-Bo, Yan, Qiu-Long, Zhang, Yue, Guo, Ruo-Chun, Meng, Jin-Xin, Ma, He, Qin, Si-Yuan, Zhu, Qing-He, Li, Chun-Qiu, Liu, Rui, Liu, Gang, Li, Sheng-Hui, Sun, Dong-Bo, Ni, Hong-Bo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Society for Microbiology 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9769736/
https://www.ncbi.nlm.nih.gov/pubmed/36321901
http://dx.doi.org/10.1128/spectrum.02211-22
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author Zhang, Xiao-Xuan
Lv, Qing-Bo
Yan, Qiu-Long
Zhang, Yue
Guo, Ruo-Chun
Meng, Jin-Xin
Ma, He
Qin, Si-Yuan
Zhu, Qing-He
Li, Chun-Qiu
Liu, Rui
Liu, Gang
Li, Sheng-Hui
Sun, Dong-Bo
Ni, Hong-Bo
author_facet Zhang, Xiao-Xuan
Lv, Qing-Bo
Yan, Qiu-Long
Zhang, Yue
Guo, Ruo-Chun
Meng, Jin-Xin
Ma, He
Qin, Si-Yuan
Zhu, Qing-He
Li, Chun-Qiu
Liu, Rui
Liu, Gang
Li, Sheng-Hui
Sun, Dong-Bo
Ni, Hong-Bo
author_sort Zhang, Xiao-Xuan
collection PubMed
description Most microbiome studies regarding the ruminant digestive tract have focused on the rumen microbiota, whereas only a few studies were performed on investigating the gut microbiota of ruminants, which limits our understanding of this important component. Herein, the gut microbiota of 30 Caprinae animals (sheep and goats) from six provinces in China was characterized using ultradeep (>100 Gbp per sample) metagenome shotgun sequencing. An inventory of Caprinae gut microbial species containing 5,046 metagenomic assembly genomes (MAGs) was constructed. Particularly, 2,530 of the genomes belonged to uncultured candidate species. These genomes largely expanded the genomic repository of the current microbes in the Caprinae gut. Several enzymes and biosynthetic gene clusters encoded by these Caprinae gut species were identified. In summary, our study extends the gut microbiota characteristics of Caprinae and provides a basis for future studies on animal production and animal health. IMPORTANCE We constructed a microbiota catalog containing 5,046 MAGs from Caprinae gut from six regions of China. Most of the MAGs do not overlap known databases and appear to be potentially new species. We also characterized the functional spectrum of these MAGs and analyzed the differences between different regions. Our study enriches the understanding of taxonomic, functional, and metabolic diversity of Caprinae gut microbiota. We are confident that the manuscript will be of utmost interest to a wide range of readers and be widely applied in future research.
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spelling pubmed-97697362022-12-22 A Catalog of over 5,000 Metagenome-Assembled Microbial Genomes from the Caprinae Gut Microbiota Zhang, Xiao-Xuan Lv, Qing-Bo Yan, Qiu-Long Zhang, Yue Guo, Ruo-Chun Meng, Jin-Xin Ma, He Qin, Si-Yuan Zhu, Qing-He Li, Chun-Qiu Liu, Rui Liu, Gang Li, Sheng-Hui Sun, Dong-Bo Ni, Hong-Bo Microbiol Spectr Research Article Most microbiome studies regarding the ruminant digestive tract have focused on the rumen microbiota, whereas only a few studies were performed on investigating the gut microbiota of ruminants, which limits our understanding of this important component. Herein, the gut microbiota of 30 Caprinae animals (sheep and goats) from six provinces in China was characterized using ultradeep (>100 Gbp per sample) metagenome shotgun sequencing. An inventory of Caprinae gut microbial species containing 5,046 metagenomic assembly genomes (MAGs) was constructed. Particularly, 2,530 of the genomes belonged to uncultured candidate species. These genomes largely expanded the genomic repository of the current microbes in the Caprinae gut. Several enzymes and biosynthetic gene clusters encoded by these Caprinae gut species were identified. In summary, our study extends the gut microbiota characteristics of Caprinae and provides a basis for future studies on animal production and animal health. IMPORTANCE We constructed a microbiota catalog containing 5,046 MAGs from Caprinae gut from six regions of China. Most of the MAGs do not overlap known databases and appear to be potentially new species. We also characterized the functional spectrum of these MAGs and analyzed the differences between different regions. Our study enriches the understanding of taxonomic, functional, and metabolic diversity of Caprinae gut microbiota. We are confident that the manuscript will be of utmost interest to a wide range of readers and be widely applied in future research. American Society for Microbiology 2022-11-02 /pmc/articles/PMC9769736/ /pubmed/36321901 http://dx.doi.org/10.1128/spectrum.02211-22 Text en Copyright © 2022 Zhang et al. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Research Article
Zhang, Xiao-Xuan
Lv, Qing-Bo
Yan, Qiu-Long
Zhang, Yue
Guo, Ruo-Chun
Meng, Jin-Xin
Ma, He
Qin, Si-Yuan
Zhu, Qing-He
Li, Chun-Qiu
Liu, Rui
Liu, Gang
Li, Sheng-Hui
Sun, Dong-Bo
Ni, Hong-Bo
A Catalog of over 5,000 Metagenome-Assembled Microbial Genomes from the Caprinae Gut Microbiota
title A Catalog of over 5,000 Metagenome-Assembled Microbial Genomes from the Caprinae Gut Microbiota
title_full A Catalog of over 5,000 Metagenome-Assembled Microbial Genomes from the Caprinae Gut Microbiota
title_fullStr A Catalog of over 5,000 Metagenome-Assembled Microbial Genomes from the Caprinae Gut Microbiota
title_full_unstemmed A Catalog of over 5,000 Metagenome-Assembled Microbial Genomes from the Caprinae Gut Microbiota
title_short A Catalog of over 5,000 Metagenome-Assembled Microbial Genomes from the Caprinae Gut Microbiota
title_sort catalog of over 5,000 metagenome-assembled microbial genomes from the caprinae gut microbiota
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9769736/
https://www.ncbi.nlm.nih.gov/pubmed/36321901
http://dx.doi.org/10.1128/spectrum.02211-22
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