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Serratia marcescens DUF1471-Containing Protein SrfN Is Needed for Adaptation to Acid and Oxidative Stresses

Bacteria can quickly adapt to constantly changing environments through a number of mechanisms, including secretion of secondary metabolites, peptides, and proteins. Serratia marcescens, an emerging pathogen with growing clinical importance due to its intrinsic resistance to several classes of antibi...

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Autores principales: Elistratova, Anna A., Matrosova, Lilia E., Khilyas, Irina V., Shirshikova, Tatiana V., Danilova, Iuliia V., Laikov, Alexander V., Romanova, Yulia D., Sierra-Bakhshi, Cecilia G., Sharipova, Margarita R., Bogomolnaya, Lydia M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Society for Microbiology 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9769812/
https://www.ncbi.nlm.nih.gov/pubmed/36218346
http://dx.doi.org/10.1128/msphere.00212-22
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author Elistratova, Anna A.
Matrosova, Lilia E.
Khilyas, Irina V.
Shirshikova, Tatiana V.
Danilova, Iuliia V.
Laikov, Alexander V.
Romanova, Yulia D.
Sierra-Bakhshi, Cecilia G.
Sharipova, Margarita R.
Bogomolnaya, Lydia M.
author_facet Elistratova, Anna A.
Matrosova, Lilia E.
Khilyas, Irina V.
Shirshikova, Tatiana V.
Danilova, Iuliia V.
Laikov, Alexander V.
Romanova, Yulia D.
Sierra-Bakhshi, Cecilia G.
Sharipova, Margarita R.
Bogomolnaya, Lydia M.
author_sort Elistratova, Anna A.
collection PubMed
description Bacteria can quickly adapt to constantly changing environments through a number of mechanisms, including secretion of secondary metabolites, peptides, and proteins. Serratia marcescens, an emerging pathogen with growing clinical importance due to its intrinsic resistance to several classes of antibiotics, can cause an array of infections in immunocompromised individuals. To better control the spread of S. marcescens infections, it is critical to identify additional targets for bacterial growth inhibition. We found that extracellular metabolites produced by the wild-type organism in response to peroxide exposure had a protective effect on an otherwise-H(2)O(2)-sensitive ΔmacAB indicator strain. Detailed analysis of the conditioned medium demonstrated that the protective effect was associated with a low-molecular-weight heat-sensitive and proteinase K-sensitive metabolite. Furthermore, liquid chromatography-tandem mass spectrometry analysis of the low-molecular-weight proteins present in the conditioned medium led to identification of the previously uncharacterized DUF1471-containing protein TBU67220 (SrfN). We found that loss of the srfN gene did not have an impact on the production of extracellular enzymes. However, the S. marcescens mutant lacking SrfN was significantly more sensitive to growth in medium with a low pH and to exposure to oxidative stress. Both defects were fully rescued by complementation. Thus, our results indicate that SrfN, a low-molecular-weight DUF1471-containing protein, is involved in S. marcescens SM6 adaptation to adverse environmental conditions. IMPORTANCE Serratia marcescens is ubiquitous in the environment and can survive in water, soil, plants, insects, and animals, and it can also cause infections in humans. In the face of disturbances such as oxidative or low-pH stress, bacteria adapt, survive, and recover through several mechanisms, including changes in their secretome. We show that a hydrogen peroxide-exposed S. marcescens milieu contains a small previously uncharacterized DUF1471-containing protein similar to the SrfN protein in Salmonella enterica serovar Typhimurium, and we illustrate the role of this protein in bacterial survival during acid and oxidative stresses.
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spelling pubmed-97698122022-12-22 Serratia marcescens DUF1471-Containing Protein SrfN Is Needed for Adaptation to Acid and Oxidative Stresses Elistratova, Anna A. Matrosova, Lilia E. Khilyas, Irina V. Shirshikova, Tatiana V. Danilova, Iuliia V. Laikov, Alexander V. Romanova, Yulia D. Sierra-Bakhshi, Cecilia G. Sharipova, Margarita R. Bogomolnaya, Lydia M. mSphere Research Article Bacteria can quickly adapt to constantly changing environments through a number of mechanisms, including secretion of secondary metabolites, peptides, and proteins. Serratia marcescens, an emerging pathogen with growing clinical importance due to its intrinsic resistance to several classes of antibiotics, can cause an array of infections in immunocompromised individuals. To better control the spread of S. marcescens infections, it is critical to identify additional targets for bacterial growth inhibition. We found that extracellular metabolites produced by the wild-type organism in response to peroxide exposure had a protective effect on an otherwise-H(2)O(2)-sensitive ΔmacAB indicator strain. Detailed analysis of the conditioned medium demonstrated that the protective effect was associated with a low-molecular-weight heat-sensitive and proteinase K-sensitive metabolite. Furthermore, liquid chromatography-tandem mass spectrometry analysis of the low-molecular-weight proteins present in the conditioned medium led to identification of the previously uncharacterized DUF1471-containing protein TBU67220 (SrfN). We found that loss of the srfN gene did not have an impact on the production of extracellular enzymes. However, the S. marcescens mutant lacking SrfN was significantly more sensitive to growth in medium with a low pH and to exposure to oxidative stress. Both defects were fully rescued by complementation. Thus, our results indicate that SrfN, a low-molecular-weight DUF1471-containing protein, is involved in S. marcescens SM6 adaptation to adverse environmental conditions. IMPORTANCE Serratia marcescens is ubiquitous in the environment and can survive in water, soil, plants, insects, and animals, and it can also cause infections in humans. In the face of disturbances such as oxidative or low-pH stress, bacteria adapt, survive, and recover through several mechanisms, including changes in their secretome. We show that a hydrogen peroxide-exposed S. marcescens milieu contains a small previously uncharacterized DUF1471-containing protein similar to the SrfN protein in Salmonella enterica serovar Typhimurium, and we illustrate the role of this protein in bacterial survival during acid and oxidative stresses. American Society for Microbiology 2022-10-11 /pmc/articles/PMC9769812/ /pubmed/36218346 http://dx.doi.org/10.1128/msphere.00212-22 Text en Copyright © 2022 Elistratova et al. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Research Article
Elistratova, Anna A.
Matrosova, Lilia E.
Khilyas, Irina V.
Shirshikova, Tatiana V.
Danilova, Iuliia V.
Laikov, Alexander V.
Romanova, Yulia D.
Sierra-Bakhshi, Cecilia G.
Sharipova, Margarita R.
Bogomolnaya, Lydia M.
Serratia marcescens DUF1471-Containing Protein SrfN Is Needed for Adaptation to Acid and Oxidative Stresses
title Serratia marcescens DUF1471-Containing Protein SrfN Is Needed for Adaptation to Acid and Oxidative Stresses
title_full Serratia marcescens DUF1471-Containing Protein SrfN Is Needed for Adaptation to Acid and Oxidative Stresses
title_fullStr Serratia marcescens DUF1471-Containing Protein SrfN Is Needed for Adaptation to Acid and Oxidative Stresses
title_full_unstemmed Serratia marcescens DUF1471-Containing Protein SrfN Is Needed for Adaptation to Acid and Oxidative Stresses
title_short Serratia marcescens DUF1471-Containing Protein SrfN Is Needed for Adaptation to Acid and Oxidative Stresses
title_sort serratia marcescens duf1471-containing protein srfn is needed for adaptation to acid and oxidative stresses
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9769812/
https://www.ncbi.nlm.nih.gov/pubmed/36218346
http://dx.doi.org/10.1128/msphere.00212-22
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