Cargando…

The Spatial Features and Temporal Changes in the Gut Microbiota of a Healthy Chinese Population

In this study, we aimed to understand the characteristics of the gut microbial composition in a healthy Chinese population and to evaluate if they differed across different regions. In addition, we aimed to understand the changes in the gut microbial composition over time. We collected 239 fecal sam...

Descripción completa

Detalles Bibliográficos
Autores principales: Zhang, Wen, Han, Na, Zhang, Tingting, Qiang, Yujun, Peng, Xianhui, Li, Xiuwen, Kan, Biao
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Society for Microbiology 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9769860/
https://www.ncbi.nlm.nih.gov/pubmed/36453887
http://dx.doi.org/10.1128/spectrum.01310-22
_version_ 1784854465513259008
author Zhang, Wen
Han, Na
Zhang, Tingting
Qiang, Yujun
Peng, Xianhui
Li, Xiuwen
Kan, Biao
author_facet Zhang, Wen
Han, Na
Zhang, Tingting
Qiang, Yujun
Peng, Xianhui
Li, Xiuwen
Kan, Biao
author_sort Zhang, Wen
collection PubMed
description In this study, we aimed to understand the characteristics of the gut microbial composition in a healthy Chinese population and to evaluate if they differed across different regions. In addition, we aimed to understand the changes in the gut microbial composition over time. We collected 239 fecal samples from healthy Chinese adults living in four regions and performed a 1-year time cohort study in a small population in Beijing. The Chinese gut microbiota share 34 core bacterial genera and 39 core bacterial species, which exist in all collected samples. Several disease-related microorganisms (DRMs), virulence factors, and antibiotic resistance genes were found in one or more healthy Chinese samples. Differences in gut microbiota were observed in samples from different regions, locations, individuals, and time points. Compared to other factors, time was associated with a lower degree of change in the gut microbiota. Our findings revealed spatial and temporal changes in the gut microbiota of healthy Chinese individuals. Compared to fecal microbiomes of 152 samples in the publicly released the Human Microbiome Project (HMP) project from the United States, samples in this study have higher variability in the fecal microbiome, with higher richness, Shannon diversity indices, and Pielou evenness indexes, at both the genus and species levels. The microbiota data obtained in this study will provide a detailed basis for further understanding the composition of the gut microbiota in the healthy Chinese population. IMPORTANCE China accounts for approximately 1/5th of the world’s total population. Differences in environment, ethnicity, and living habits could impart unique features to the structure of the gut microbiota of Chinese individuals. In 2016, we started to investigate healthy Chinese people and their gut microbiomes. Phase I results for 16S rRNA amplicons have been released. However, owing to the limitations of 16S rRNA amplicon sequencing, the gut microbiome of a healthy Chinese population could not be examined thoroughly at the species level, and the detailed changes in the gut microbiota over time need to be investigated. To address these knowledge gaps, we started a phase II study and investigated the basis for variations in the gut microbiome composition in a healthy Chinese population at the species level using shotgun metagenomics technology. In the phase II study, we also conducted a time scale analysis of fecal samples from healthy Chinese subjects, as a pioneered study, which quantitatively clarified the changes in the gut microbiota at both the spatial and temporal levels and elucidated the distribution pattern of DRMs in healthy Chinese individuals.
format Online
Article
Text
id pubmed-9769860
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher American Society for Microbiology
record_format MEDLINE/PubMed
spelling pubmed-97698602022-12-22 The Spatial Features and Temporal Changes in the Gut Microbiota of a Healthy Chinese Population Zhang, Wen Han, Na Zhang, Tingting Qiang, Yujun Peng, Xianhui Li, Xiuwen Kan, Biao Microbiol Spectr Research Article In this study, we aimed to understand the characteristics of the gut microbial composition in a healthy Chinese population and to evaluate if they differed across different regions. In addition, we aimed to understand the changes in the gut microbial composition over time. We collected 239 fecal samples from healthy Chinese adults living in four regions and performed a 1-year time cohort study in a small population in Beijing. The Chinese gut microbiota share 34 core bacterial genera and 39 core bacterial species, which exist in all collected samples. Several disease-related microorganisms (DRMs), virulence factors, and antibiotic resistance genes were found in one or more healthy Chinese samples. Differences in gut microbiota were observed in samples from different regions, locations, individuals, and time points. Compared to other factors, time was associated with a lower degree of change in the gut microbiota. Our findings revealed spatial and temporal changes in the gut microbiota of healthy Chinese individuals. Compared to fecal microbiomes of 152 samples in the publicly released the Human Microbiome Project (HMP) project from the United States, samples in this study have higher variability in the fecal microbiome, with higher richness, Shannon diversity indices, and Pielou evenness indexes, at both the genus and species levels. The microbiota data obtained in this study will provide a detailed basis for further understanding the composition of the gut microbiota in the healthy Chinese population. IMPORTANCE China accounts for approximately 1/5th of the world’s total population. Differences in environment, ethnicity, and living habits could impart unique features to the structure of the gut microbiota of Chinese individuals. In 2016, we started to investigate healthy Chinese people and their gut microbiomes. Phase I results for 16S rRNA amplicons have been released. However, owing to the limitations of 16S rRNA amplicon sequencing, the gut microbiome of a healthy Chinese population could not be examined thoroughly at the species level, and the detailed changes in the gut microbiota over time need to be investigated. To address these knowledge gaps, we started a phase II study and investigated the basis for variations in the gut microbiome composition in a healthy Chinese population at the species level using shotgun metagenomics technology. In the phase II study, we also conducted a time scale analysis of fecal samples from healthy Chinese subjects, as a pioneered study, which quantitatively clarified the changes in the gut microbiota at both the spatial and temporal levels and elucidated the distribution pattern of DRMs in healthy Chinese individuals. American Society for Microbiology 2022-12-01 /pmc/articles/PMC9769860/ /pubmed/36453887 http://dx.doi.org/10.1128/spectrum.01310-22 Text en Copyright © 2022 Zhang et al. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Research Article
Zhang, Wen
Han, Na
Zhang, Tingting
Qiang, Yujun
Peng, Xianhui
Li, Xiuwen
Kan, Biao
The Spatial Features and Temporal Changes in the Gut Microbiota of a Healthy Chinese Population
title The Spatial Features and Temporal Changes in the Gut Microbiota of a Healthy Chinese Population
title_full The Spatial Features and Temporal Changes in the Gut Microbiota of a Healthy Chinese Population
title_fullStr The Spatial Features and Temporal Changes in the Gut Microbiota of a Healthy Chinese Population
title_full_unstemmed The Spatial Features and Temporal Changes in the Gut Microbiota of a Healthy Chinese Population
title_short The Spatial Features and Temporal Changes in the Gut Microbiota of a Healthy Chinese Population
title_sort spatial features and temporal changes in the gut microbiota of a healthy chinese population
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9769860/
https://www.ncbi.nlm.nih.gov/pubmed/36453887
http://dx.doi.org/10.1128/spectrum.01310-22
work_keys_str_mv AT zhangwen thespatialfeaturesandtemporalchangesinthegutmicrobiotaofahealthychinesepopulation
AT hanna thespatialfeaturesandtemporalchangesinthegutmicrobiotaofahealthychinesepopulation
AT zhangtingting thespatialfeaturesandtemporalchangesinthegutmicrobiotaofahealthychinesepopulation
AT qiangyujun thespatialfeaturesandtemporalchangesinthegutmicrobiotaofahealthychinesepopulation
AT pengxianhui thespatialfeaturesandtemporalchangesinthegutmicrobiotaofahealthychinesepopulation
AT lixiuwen thespatialfeaturesandtemporalchangesinthegutmicrobiotaofahealthychinesepopulation
AT kanbiao thespatialfeaturesandtemporalchangesinthegutmicrobiotaofahealthychinesepopulation
AT zhangwen spatialfeaturesandtemporalchangesinthegutmicrobiotaofahealthychinesepopulation
AT hanna spatialfeaturesandtemporalchangesinthegutmicrobiotaofahealthychinesepopulation
AT zhangtingting spatialfeaturesandtemporalchangesinthegutmicrobiotaofahealthychinesepopulation
AT qiangyujun spatialfeaturesandtemporalchangesinthegutmicrobiotaofahealthychinesepopulation
AT pengxianhui spatialfeaturesandtemporalchangesinthegutmicrobiotaofahealthychinesepopulation
AT lixiuwen spatialfeaturesandtemporalchangesinthegutmicrobiotaofahealthychinesepopulation
AT kanbiao spatialfeaturesandtemporalchangesinthegutmicrobiotaofahealthychinesepopulation