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Use of a rapid digital microfluidics-powered immunoassay for assessing measles and rubella infection and immunity in outbreak settings in the Democratic Republic of the Congo

The Democratic Republic of the Congo (DRC) has a high measles incidence despite elimination efforts and has yet to introduce rubella vaccine. We evaluated the performance of a prototype rapid digital microfluidics powered (DMF) enzyme-linked immunoassay (ELISA) assessing measles and rubella infectio...

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Autores principales: Knipes, Alaine K., Summers, Aimee, Sklavounos, Alexandros A., Lamanna, Julian, de Campos, Richard P. S., Narahari, Tanya, Dixon, Christopher, Fobel, Ryan, Ndjakani, Yassa D., Lubula, Leopold, Magazani, Alain, Muyembe, Jean Jacques, Lay, Yvonne, Pukuta, Elizabeth, Waku-Kouomou, Diane, Hao, Lijuan, Kayembe, Jolie Kasongo, Fobel, Christian, Dahmer, Joshua, Lee, Adam, Ho, Man, Valenzuela, Jose Gilberto Camacho, Rackus, Darius G., Shih, Roger, Seale, Brendon, Chang, Ann, Paluku, Gilson, Rota, Paul A., Wheeler, Aaron R., Scobie, Heather M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9770344/
https://www.ncbi.nlm.nih.gov/pubmed/36542608
http://dx.doi.org/10.1371/journal.pone.0278749
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author Knipes, Alaine K.
Summers, Aimee
Sklavounos, Alexandros A.
Lamanna, Julian
de Campos, Richard P. S.
Narahari, Tanya
Dixon, Christopher
Fobel, Ryan
Ndjakani, Yassa D.
Lubula, Leopold
Magazani, Alain
Muyembe, Jean Jacques
Lay, Yvonne
Pukuta, Elizabeth
Waku-Kouomou, Diane
Hao, Lijuan
Kayembe, Jolie Kasongo
Fobel, Christian
Dahmer, Joshua
Lee, Adam
Ho, Man
Valenzuela, Jose Gilberto Camacho
Rackus, Darius G.
Shih, Roger
Seale, Brendon
Chang, Ann
Paluku, Gilson
Rota, Paul A.
Wheeler, Aaron R.
Scobie, Heather M.
author_facet Knipes, Alaine K.
Summers, Aimee
Sklavounos, Alexandros A.
Lamanna, Julian
de Campos, Richard P. S.
Narahari, Tanya
Dixon, Christopher
Fobel, Ryan
Ndjakani, Yassa D.
Lubula, Leopold
Magazani, Alain
Muyembe, Jean Jacques
Lay, Yvonne
Pukuta, Elizabeth
Waku-Kouomou, Diane
Hao, Lijuan
Kayembe, Jolie Kasongo
Fobel, Christian
Dahmer, Joshua
Lee, Adam
Ho, Man
Valenzuela, Jose Gilberto Camacho
Rackus, Darius G.
Shih, Roger
Seale, Brendon
Chang, Ann
Paluku, Gilson
Rota, Paul A.
Wheeler, Aaron R.
Scobie, Heather M.
author_sort Knipes, Alaine K.
collection PubMed
description The Democratic Republic of the Congo (DRC) has a high measles incidence despite elimination efforts and has yet to introduce rubella vaccine. We evaluated the performance of a prototype rapid digital microfluidics powered (DMF) enzyme-linked immunoassay (ELISA) assessing measles and rubella infection, by testing for immunoglobulin M (IgM), and immunity from natural infection or vaccine, by testing immunoglobulin G (IgG), in outbreak settings. Field evaluations were conducted during September 2017, in Kinshasa province, DRC. Blood specimens were collected during an outbreak investigation of suspected measles cases and tested for measles and rubella IgM and IgG using the DMF-ELISA in the field. Simultaneously, a household serosurvey for measles and rubella IgG was conducted in a recently confirmed measles outbreak area. DMF-ELISA results were compared with reference ELISA results tested at DRC’s National Public Health Laboratory and the US Centers for Disease Control and Prevention. Of 157 suspected measles cases, rubella IgM was detected in 54% while measles IgM was detected in 13%. Measles IgG-positive cases were higher among vaccinated persons (87%) than unvaccinated persons (72%). In the recent measles outbreak area, measles IgG seroprevalence was 93% overall, while rubella seroprevalence was lower for children (77%) than women (98%). Compared with reference ELISA, DMF-ELISA sensitivity and specificity were 82% and 78% for measles IgG; 88% and 89% for measles IgM; 85% and 85% for rubella IgG; and 81% and 83% for rubella IgM, respectively. Rubella infection was detected in more than half of persons meeting the suspected measles case definition during a presumed measles outbreak, suggesting substantial unrecognized rubella incidence, and highlighting the need for rubella vaccine introduction into the national schedule. The performance of the DMF-ELISA suggested that this technology can be used to develop rapid diagnostic tests for measles and rubella.
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spelling pubmed-97703442022-12-22 Use of a rapid digital microfluidics-powered immunoassay for assessing measles and rubella infection and immunity in outbreak settings in the Democratic Republic of the Congo Knipes, Alaine K. Summers, Aimee Sklavounos, Alexandros A. Lamanna, Julian de Campos, Richard P. S. Narahari, Tanya Dixon, Christopher Fobel, Ryan Ndjakani, Yassa D. Lubula, Leopold Magazani, Alain Muyembe, Jean Jacques Lay, Yvonne Pukuta, Elizabeth Waku-Kouomou, Diane Hao, Lijuan Kayembe, Jolie Kasongo Fobel, Christian Dahmer, Joshua Lee, Adam Ho, Man Valenzuela, Jose Gilberto Camacho Rackus, Darius G. Shih, Roger Seale, Brendon Chang, Ann Paluku, Gilson Rota, Paul A. Wheeler, Aaron R. Scobie, Heather M. PLoS One Research Article The Democratic Republic of the Congo (DRC) has a high measles incidence despite elimination efforts and has yet to introduce rubella vaccine. We evaluated the performance of a prototype rapid digital microfluidics powered (DMF) enzyme-linked immunoassay (ELISA) assessing measles and rubella infection, by testing for immunoglobulin M (IgM), and immunity from natural infection or vaccine, by testing immunoglobulin G (IgG), in outbreak settings. Field evaluations were conducted during September 2017, in Kinshasa province, DRC. Blood specimens were collected during an outbreak investigation of suspected measles cases and tested for measles and rubella IgM and IgG using the DMF-ELISA in the field. Simultaneously, a household serosurvey for measles and rubella IgG was conducted in a recently confirmed measles outbreak area. DMF-ELISA results were compared with reference ELISA results tested at DRC’s National Public Health Laboratory and the US Centers for Disease Control and Prevention. Of 157 suspected measles cases, rubella IgM was detected in 54% while measles IgM was detected in 13%. Measles IgG-positive cases were higher among vaccinated persons (87%) than unvaccinated persons (72%). In the recent measles outbreak area, measles IgG seroprevalence was 93% overall, while rubella seroprevalence was lower for children (77%) than women (98%). Compared with reference ELISA, DMF-ELISA sensitivity and specificity were 82% and 78% for measles IgG; 88% and 89% for measles IgM; 85% and 85% for rubella IgG; and 81% and 83% for rubella IgM, respectively. Rubella infection was detected in more than half of persons meeting the suspected measles case definition during a presumed measles outbreak, suggesting substantial unrecognized rubella incidence, and highlighting the need for rubella vaccine introduction into the national schedule. The performance of the DMF-ELISA suggested that this technology can be used to develop rapid diagnostic tests for measles and rubella. Public Library of Science 2022-12-21 /pmc/articles/PMC9770344/ /pubmed/36542608 http://dx.doi.org/10.1371/journal.pone.0278749 Text en https://creativecommons.org/publicdomain/zero/1.0/This is an open access article, free of all copyright, and may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose. The work is made available under the Creative Commons CC0 (https://creativecommons.org/publicdomain/zero/1.0/) public domain dedication.
spellingShingle Research Article
Knipes, Alaine K.
Summers, Aimee
Sklavounos, Alexandros A.
Lamanna, Julian
de Campos, Richard P. S.
Narahari, Tanya
Dixon, Christopher
Fobel, Ryan
Ndjakani, Yassa D.
Lubula, Leopold
Magazani, Alain
Muyembe, Jean Jacques
Lay, Yvonne
Pukuta, Elizabeth
Waku-Kouomou, Diane
Hao, Lijuan
Kayembe, Jolie Kasongo
Fobel, Christian
Dahmer, Joshua
Lee, Adam
Ho, Man
Valenzuela, Jose Gilberto Camacho
Rackus, Darius G.
Shih, Roger
Seale, Brendon
Chang, Ann
Paluku, Gilson
Rota, Paul A.
Wheeler, Aaron R.
Scobie, Heather M.
Use of a rapid digital microfluidics-powered immunoassay for assessing measles and rubella infection and immunity in outbreak settings in the Democratic Republic of the Congo
title Use of a rapid digital microfluidics-powered immunoassay for assessing measles and rubella infection and immunity in outbreak settings in the Democratic Republic of the Congo
title_full Use of a rapid digital microfluidics-powered immunoassay for assessing measles and rubella infection and immunity in outbreak settings in the Democratic Republic of the Congo
title_fullStr Use of a rapid digital microfluidics-powered immunoassay for assessing measles and rubella infection and immunity in outbreak settings in the Democratic Republic of the Congo
title_full_unstemmed Use of a rapid digital microfluidics-powered immunoassay for assessing measles and rubella infection and immunity in outbreak settings in the Democratic Republic of the Congo
title_short Use of a rapid digital microfluidics-powered immunoassay for assessing measles and rubella infection and immunity in outbreak settings in the Democratic Republic of the Congo
title_sort use of a rapid digital microfluidics-powered immunoassay for assessing measles and rubella infection and immunity in outbreak settings in the democratic republic of the congo
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9770344/
https://www.ncbi.nlm.nih.gov/pubmed/36542608
http://dx.doi.org/10.1371/journal.pone.0278749
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