Hydrogel Derived from Glucomannan-Chitosan to Improve the Survival of Lactobacillus acidophilus FNCC 0051 in Simulated Gastrointestinal Fluid

The probiotic encapsulating hydrogel derived from porang (Amorphophallus oncophyllus) glucomannan and chitosan was investigated with regard to its encapsulation efficiency, physical properties, prebiotic activity, and survival under simulated gastrointestinal conditions. The hydrogel's encapsulation efficiency was improved by varying the number of the Lactobacillus acidophilus FNCC 0051, which also served to increase the diameter (2-3 mm), polydispersity index (1.23–1.65), positive zeta potential, whiteness, and brightness of the hydrogel. Moreover, the hydrogel's prebiotic activity score was higher than that of inulin after 24 h of incubation, reflecting its role as a cell encapsulant, particularly when it comes to maintaining cells during exposure to simulated gastrointestinal fluid. The cell viability increased from 86% to 100% when immersed in intestinal juice, which is comparable to the increase achieved using alginate and konjac glucomannan hydrogels. Future animal studies are required to determine the cell viability in actual gastrointestinal conditions and assess the health effects of the hydrogel.