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Single-cell transcriptome reveals core cell populations and androgen-RXFP2 axis involved in deer antler full regeneration
Deer antlers constitute a unique mammalian model for the study of both organ formation in postnatal life and annual full regeneration. Previous studies revealed that these events are achieved through the proliferation and differentiation of antlerogenic periosteum (AP) cells and pedicle periosteum (...
Autores principales: | , , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Springer Nature Singapore
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9772379/ https://www.ncbi.nlm.nih.gov/pubmed/36542206 http://dx.doi.org/10.1186/s13619-022-00153-4 |
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author | Ba, Hengxing Wang, Xin Wang, Datao Ren, Jing Wang, Zhen Sun, Hai-Xi Hu, Pengfei Zhang, Guokun Wang, Shengnan Ma, Chao Wang, Yusu Wang, Enpeng Chen, Liang Liu, Tianbin Gu, Ying Li, Chunyi |
author_facet | Ba, Hengxing Wang, Xin Wang, Datao Ren, Jing Wang, Zhen Sun, Hai-Xi Hu, Pengfei Zhang, Guokun Wang, Shengnan Ma, Chao Wang, Yusu Wang, Enpeng Chen, Liang Liu, Tianbin Gu, Ying Li, Chunyi |
author_sort | Ba, Hengxing |
collection | PubMed |
description | Deer antlers constitute a unique mammalian model for the study of both organ formation in postnatal life and annual full regeneration. Previous studies revealed that these events are achieved through the proliferation and differentiation of antlerogenic periosteum (AP) cells and pedicle periosteum (PP) cells, respectively. As the cells resident in the AP and the PP possess stem cell attributes, both antler generation and regeneration are stem cell-based processes. However, the cell composition of each tissue type and molecular events underlying antler development remain poorly characterized. Here, we took the approach of single-cell RNA sequencing (scRNA-Seq) and identified eight cell types (mainly THY1(+) cells, progenitor cells, and osteochondroblasts) and three core subclusters of the THY1(+) cells (SC2, SC3, and SC4). Endothelial and mural cells each are heterogeneous at transcriptional level. It was the proliferation of progenitor, mural, and endothelial cells in the activated antler-lineage-specific tissues that drove the rapid formation of the antler. We detected the differences in the initial differentiation process between antler generation and regeneration using pseudotime trajectory analysis. These may be due to the difference in the degree of stemness of the AP-THY1(+) and PP-THY1(+) cells. We further found that androgen-RXFP2 axis may be involved in triggering initial antler full regeneration. Fully deciphering the cell composition for these antler tissue types will open up new avenues for elucidating the mechanism underlying antler full renewal in specific and regenerative medicine in general. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s13619-022-00153-4. |
format | Online Article Text |
id | pubmed-9772379 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Springer Nature Singapore |
record_format | MEDLINE/PubMed |
spelling | pubmed-97723792023-01-17 Single-cell transcriptome reveals core cell populations and androgen-RXFP2 axis involved in deer antler full regeneration Ba, Hengxing Wang, Xin Wang, Datao Ren, Jing Wang, Zhen Sun, Hai-Xi Hu, Pengfei Zhang, Guokun Wang, Shengnan Ma, Chao Wang, Yusu Wang, Enpeng Chen, Liang Liu, Tianbin Gu, Ying Li, Chunyi Cell Regen Research Article Deer antlers constitute a unique mammalian model for the study of both organ formation in postnatal life and annual full regeneration. Previous studies revealed that these events are achieved through the proliferation and differentiation of antlerogenic periosteum (AP) cells and pedicle periosteum (PP) cells, respectively. As the cells resident in the AP and the PP possess stem cell attributes, both antler generation and regeneration are stem cell-based processes. However, the cell composition of each tissue type and molecular events underlying antler development remain poorly characterized. Here, we took the approach of single-cell RNA sequencing (scRNA-Seq) and identified eight cell types (mainly THY1(+) cells, progenitor cells, and osteochondroblasts) and three core subclusters of the THY1(+) cells (SC2, SC3, and SC4). Endothelial and mural cells each are heterogeneous at transcriptional level. It was the proliferation of progenitor, mural, and endothelial cells in the activated antler-lineage-specific tissues that drove the rapid formation of the antler. We detected the differences in the initial differentiation process between antler generation and regeneration using pseudotime trajectory analysis. These may be due to the difference in the degree of stemness of the AP-THY1(+) and PP-THY1(+) cells. We further found that androgen-RXFP2 axis may be involved in triggering initial antler full regeneration. Fully deciphering the cell composition for these antler tissue types will open up new avenues for elucidating the mechanism underlying antler full renewal in specific and regenerative medicine in general. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s13619-022-00153-4. Springer Nature Singapore 2022-12-21 /pmc/articles/PMC9772379/ /pubmed/36542206 http://dx.doi.org/10.1186/s13619-022-00153-4 Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated in a credit line to the data. |
spellingShingle | Research Article Ba, Hengxing Wang, Xin Wang, Datao Ren, Jing Wang, Zhen Sun, Hai-Xi Hu, Pengfei Zhang, Guokun Wang, Shengnan Ma, Chao Wang, Yusu Wang, Enpeng Chen, Liang Liu, Tianbin Gu, Ying Li, Chunyi Single-cell transcriptome reveals core cell populations and androgen-RXFP2 axis involved in deer antler full regeneration |
title | Single-cell transcriptome reveals core cell populations and androgen-RXFP2 axis involved in deer antler full regeneration |
title_full | Single-cell transcriptome reveals core cell populations and androgen-RXFP2 axis involved in deer antler full regeneration |
title_fullStr | Single-cell transcriptome reveals core cell populations and androgen-RXFP2 axis involved in deer antler full regeneration |
title_full_unstemmed | Single-cell transcriptome reveals core cell populations and androgen-RXFP2 axis involved in deer antler full regeneration |
title_short | Single-cell transcriptome reveals core cell populations and androgen-RXFP2 axis involved in deer antler full regeneration |
title_sort | single-cell transcriptome reveals core cell populations and androgen-rxfp2 axis involved in deer antler full regeneration |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9772379/ https://www.ncbi.nlm.nih.gov/pubmed/36542206 http://dx.doi.org/10.1186/s13619-022-00153-4 |
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