A cell-based bioluminescence reporter assay of human Sonic Hedgehog protein autoprocessing to identify inhibitors and activators

The Sonic Hedgehog (SHh) precursor protein undergoes biosynthetic autoprocessing to cleave off and covalently attach cholesterol to the SHh signaling ligand, a vital morphogen and oncogenic effector protein. Autoprocessing is self-catalyzed by SHhC, the SHh precursor’s C-terminal enzymatic domain. A...

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Detalles Bibliográficos
Autores principales: Ciulla, Daniel A., Dranchak, Patricia, Pezzullo, John L., Mancusi, Rebecca A., Psaras, Alexandra Maria, Rai, Ganesha, Giner, José-Luis, Inglese, James, Callahan, Brian P.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Society for Biochemistry and Molecular Biology 2022
Materias:
Methods and Resources
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9772569/
https://www.ncbi.nlm.nih.gov/pubmed/36400200
http://dx.doi.org/10.1016/j.jbc.2022.102705

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