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Metagenomic next-generation sequencing shotgun for the diagnosis of infection in connective tissue diseases: A retrospective study
OBJECTIVE: Patients with connective tissue diseases (CTDs) are at high risk of infection due to various reasons. The purpose of the study was to investigate the infection diagnosis value of metagenomic next-generation sequencing (mNGS) shotgun in CTDs to guide the use of anti-infective therapy more...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9772835/ https://www.ncbi.nlm.nih.gov/pubmed/36569204 http://dx.doi.org/10.3389/fcimb.2022.865637 |
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author | Wang, Huyan Shi, Xiaowei Yang, Huanhuan Du, Yan Xue, Jing |
author_facet | Wang, Huyan Shi, Xiaowei Yang, Huanhuan Du, Yan Xue, Jing |
author_sort | Wang, Huyan |
collection | PubMed |
description | OBJECTIVE: Patients with connective tissue diseases (CTDs) are at high risk of infection due to various reasons. The purpose of the study was to investigate the infection diagnosis value of metagenomic next-generation sequencing (mNGS) shotgun in CTDs to guide the use of anti-infective therapy more quickly and accurately. METHODS: In this retrospective study, a total of 103 patients with CTDs admitted with suspected infection between December 2018 and September 2021 were assessed using mNGS as well as conventional microbiological tests (CMT). RESULTS: Among these 103 patients, 65 were confirmed to have an infection (Group I) and 38 had no infection (Group II). mNGS reached a sensitivity of 92.31% in diagnosing pathogens in Group I. Moreover, mNGS showed good performance in identifying mixed infection. In all infection types, lung infection was the most common. mNGS also played an important role in detecting Pneumocystis jirovecii, which was associated with low CD4+ T-cell counts inextricably. CONCLUSION: mNGS is a useful tool with outstanding diagnostic potential in identifying pathogens in patients with CTDs and conduce to provide guidance in clinical practice. |
format | Online Article Text |
id | pubmed-9772835 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-97728352022-12-23 Metagenomic next-generation sequencing shotgun for the diagnosis of infection in connective tissue diseases: A retrospective study Wang, Huyan Shi, Xiaowei Yang, Huanhuan Du, Yan Xue, Jing Front Cell Infect Microbiol Cellular and Infection Microbiology OBJECTIVE: Patients with connective tissue diseases (CTDs) are at high risk of infection due to various reasons. The purpose of the study was to investigate the infection diagnosis value of metagenomic next-generation sequencing (mNGS) shotgun in CTDs to guide the use of anti-infective therapy more quickly and accurately. METHODS: In this retrospective study, a total of 103 patients with CTDs admitted with suspected infection between December 2018 and September 2021 were assessed using mNGS as well as conventional microbiological tests (CMT). RESULTS: Among these 103 patients, 65 were confirmed to have an infection (Group I) and 38 had no infection (Group II). mNGS reached a sensitivity of 92.31% in diagnosing pathogens in Group I. Moreover, mNGS showed good performance in identifying mixed infection. In all infection types, lung infection was the most common. mNGS also played an important role in detecting Pneumocystis jirovecii, which was associated with low CD4+ T-cell counts inextricably. CONCLUSION: mNGS is a useful tool with outstanding diagnostic potential in identifying pathogens in patients with CTDs and conduce to provide guidance in clinical practice. Frontiers Media S.A. 2022-12-08 /pmc/articles/PMC9772835/ /pubmed/36569204 http://dx.doi.org/10.3389/fcimb.2022.865637 Text en Copyright © 2022 Wang, Shi, Yang, Du and Xue https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Cellular and Infection Microbiology Wang, Huyan Shi, Xiaowei Yang, Huanhuan Du, Yan Xue, Jing Metagenomic next-generation sequencing shotgun for the diagnosis of infection in connective tissue diseases: A retrospective study |
title | Metagenomic next-generation sequencing shotgun for the diagnosis of infection in connective tissue diseases: A retrospective study |
title_full | Metagenomic next-generation sequencing shotgun for the diagnosis of infection in connective tissue diseases: A retrospective study |
title_fullStr | Metagenomic next-generation sequencing shotgun for the diagnosis of infection in connective tissue diseases: A retrospective study |
title_full_unstemmed | Metagenomic next-generation sequencing shotgun for the diagnosis of infection in connective tissue diseases: A retrospective study |
title_short | Metagenomic next-generation sequencing shotgun for the diagnosis of infection in connective tissue diseases: A retrospective study |
title_sort | metagenomic next-generation sequencing shotgun for the diagnosis of infection in connective tissue diseases: a retrospective study |
topic | Cellular and Infection Microbiology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9772835/ https://www.ncbi.nlm.nih.gov/pubmed/36569204 http://dx.doi.org/10.3389/fcimb.2022.865637 |
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