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Gut microbiota profiling variated during colorectal cancer development in mouse
BACKGROUND: The imbalance of intestinal flora may promote the occurrence and development of colorectal cancer, changes of the intestinal flora during the development of colorectal cancer and the mechanism that promotes the colorectal cancer were discovered in this study. Deep sequencing of the micro...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9773433/ https://www.ncbi.nlm.nih.gov/pubmed/36550412 http://dx.doi.org/10.1186/s12864-022-09008-3 |
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author | Liu, Jingjing Dong, Wei Zhao, Jian Wu, Jing Xia, Jinqiang Xie, Shaofei Song, Xiaofeng |
author_facet | Liu, Jingjing Dong, Wei Zhao, Jian Wu, Jing Xia, Jinqiang Xie, Shaofei Song, Xiaofeng |
author_sort | Liu, Jingjing |
collection | PubMed |
description | BACKGROUND: The imbalance of intestinal flora may promote the occurrence and development of colorectal cancer, changes of the intestinal flora during the development of colorectal cancer and the mechanism that promotes the colorectal cancer were discovered in this study. Deep sequencing of the microbial 16 s ribosomal RNA gene was used to investigate alterations in feces samples of mice at the early inflammation stage and fully developed stage of colorectal cancer. RESULTS: According to PCoA analysis and ANOSIM test, we found the intestinal flora had significantly changed in mice with colorectal inflammation or colorectal cancer compared with healthy mice (p < 0.05). Using correlation analysis, we found that Muribaculaceae and Bacteroidaceae had strong excluding interactions. The functional changes of the gut microbiota include the up-regulation of the cancers pathway and the down-regulation of the replication and repair pathways. CONCLUSION: Our study found the intestinal flora of mice suffering from colorectal inflammation and colorectal cancer has changed significantly, especially the decrease of Muribaculaceae and the increase of Bacteroidaceae. We suppose that these two floras may play an important role in development of colorectal cancer. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12864-022-09008-3. |
format | Online Article Text |
id | pubmed-9773433 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-97734332022-12-23 Gut microbiota profiling variated during colorectal cancer development in mouse Liu, Jingjing Dong, Wei Zhao, Jian Wu, Jing Xia, Jinqiang Xie, Shaofei Song, Xiaofeng BMC Genomics Research BACKGROUND: The imbalance of intestinal flora may promote the occurrence and development of colorectal cancer, changes of the intestinal flora during the development of colorectal cancer and the mechanism that promotes the colorectal cancer were discovered in this study. Deep sequencing of the microbial 16 s ribosomal RNA gene was used to investigate alterations in feces samples of mice at the early inflammation stage and fully developed stage of colorectal cancer. RESULTS: According to PCoA analysis and ANOSIM test, we found the intestinal flora had significantly changed in mice with colorectal inflammation or colorectal cancer compared with healthy mice (p < 0.05). Using correlation analysis, we found that Muribaculaceae and Bacteroidaceae had strong excluding interactions. The functional changes of the gut microbiota include the up-regulation of the cancers pathway and the down-regulation of the replication and repair pathways. CONCLUSION: Our study found the intestinal flora of mice suffering from colorectal inflammation and colorectal cancer has changed significantly, especially the decrease of Muribaculaceae and the increase of Bacteroidaceae. We suppose that these two floras may play an important role in development of colorectal cancer. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12864-022-09008-3. BioMed Central 2022-12-22 /pmc/articles/PMC9773433/ /pubmed/36550412 http://dx.doi.org/10.1186/s12864-022-09008-3 Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated in a credit line to the data. |
spellingShingle | Research Liu, Jingjing Dong, Wei Zhao, Jian Wu, Jing Xia, Jinqiang Xie, Shaofei Song, Xiaofeng Gut microbiota profiling variated during colorectal cancer development in mouse |
title | Gut microbiota profiling variated during colorectal cancer development in mouse |
title_full | Gut microbiota profiling variated during colorectal cancer development in mouse |
title_fullStr | Gut microbiota profiling variated during colorectal cancer development in mouse |
title_full_unstemmed | Gut microbiota profiling variated during colorectal cancer development in mouse |
title_short | Gut microbiota profiling variated during colorectal cancer development in mouse |
title_sort | gut microbiota profiling variated during colorectal cancer development in mouse |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9773433/ https://www.ncbi.nlm.nih.gov/pubmed/36550412 http://dx.doi.org/10.1186/s12864-022-09008-3 |
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