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Diversity of bacterial communities in the plasmodia of myxomycetes

BACKGROUND: Myxomycetes are a group of eukaryotes belonging to Amoebozoa, which are characterized by a distinctive life cycle, including the plasmodium stage and fruit body stage. Plasmodia are all found to be associated with bacteria. However, the information about bacteria diversity and compositio...

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Autores principales: Li, Shu, Qi, Bao, Wang, Wan, Peng, Xueyan, Gontcharov, Andrey A, Liu, Bao, Wang, Qi, Li, Yu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9773492/
https://www.ncbi.nlm.nih.gov/pubmed/36544088
http://dx.doi.org/10.1186/s12866-022-02725-5
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author Li, Shu
Qi, Bao
Wang, Wan
Peng, Xueyan
Gontcharov, Andrey A
Liu, Bao
Wang, Qi
Li, Yu
author_facet Li, Shu
Qi, Bao
Wang, Wan
Peng, Xueyan
Gontcharov, Andrey A
Liu, Bao
Wang, Qi
Li, Yu
author_sort Li, Shu
collection PubMed
description BACKGROUND: Myxomycetes are a group of eukaryotes belonging to Amoebozoa, which are characterized by a distinctive life cycle, including the plasmodium stage and fruit body stage. Plasmodia are all found to be associated with bacteria. However, the information about bacteria diversity and composition in different plasmodia was limited. Therefore, this study aimed to investigate the bacterial diversity of plasmodia from different myxomycetes species and reveal the potential function of plasmodia-associated bacterial communities. RESULTS: The bacterial communities associated with the plasmodia of six myxomycetes (Didymium iridis, Didymium squamulosum, Diderma hemisphaericum, Lepidoderma tigrinum, Fuligo leviderma, and Physarum melleum) were identified by 16S rRNA amplicon sequencing. The six plasmodia harbored 38 to 52 bacterial operational taxonomic units (OTUs) that belonged to 7 phyla, 16 classes, 23 orders, 40 families, and 53 genera. The dominant phyla were Bacteroidetes, Firmicutes, and Proteobacteria. Most OTUs were shared among the six myxomycetes, while unique bacteria in each species only accounted for a tiny proportion of the total OTUs. CONCLUSIONS: Although each of the six myxomycetes plasmodia had different bacterial community compositions, a high similarity was observed in the plasmodia-associated bacterial communities' functional composition. The high enrichment for gram-negative (> 90%) and aerobic (> 99%) bacteria in plasmodia suggest that myxomycetes may positively recruit certain kinds of bacteria from the surrounding environment. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12866-022-02725-5.
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spelling pubmed-97734922022-12-23 Diversity of bacterial communities in the plasmodia of myxomycetes Li, Shu Qi, Bao Wang, Wan Peng, Xueyan Gontcharov, Andrey A Liu, Bao Wang, Qi Li, Yu BMC Microbiol Research BACKGROUND: Myxomycetes are a group of eukaryotes belonging to Amoebozoa, which are characterized by a distinctive life cycle, including the plasmodium stage and fruit body stage. Plasmodia are all found to be associated with bacteria. However, the information about bacteria diversity and composition in different plasmodia was limited. Therefore, this study aimed to investigate the bacterial diversity of plasmodia from different myxomycetes species and reveal the potential function of plasmodia-associated bacterial communities. RESULTS: The bacterial communities associated with the plasmodia of six myxomycetes (Didymium iridis, Didymium squamulosum, Diderma hemisphaericum, Lepidoderma tigrinum, Fuligo leviderma, and Physarum melleum) were identified by 16S rRNA amplicon sequencing. The six plasmodia harbored 38 to 52 bacterial operational taxonomic units (OTUs) that belonged to 7 phyla, 16 classes, 23 orders, 40 families, and 53 genera. The dominant phyla were Bacteroidetes, Firmicutes, and Proteobacteria. Most OTUs were shared among the six myxomycetes, while unique bacteria in each species only accounted for a tiny proportion of the total OTUs. CONCLUSIONS: Although each of the six myxomycetes plasmodia had different bacterial community compositions, a high similarity was observed in the plasmodia-associated bacterial communities' functional composition. The high enrichment for gram-negative (> 90%) and aerobic (> 99%) bacteria in plasmodia suggest that myxomycetes may positively recruit certain kinds of bacteria from the surrounding environment. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12866-022-02725-5. BioMed Central 2022-12-22 /pmc/articles/PMC9773492/ /pubmed/36544088 http://dx.doi.org/10.1186/s12866-022-02725-5 Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
spellingShingle Research
Li, Shu
Qi, Bao
Wang, Wan
Peng, Xueyan
Gontcharov, Andrey A
Liu, Bao
Wang, Qi
Li, Yu
Diversity of bacterial communities in the plasmodia of myxomycetes
title Diversity of bacterial communities in the plasmodia of myxomycetes
title_full Diversity of bacterial communities in the plasmodia of myxomycetes
title_fullStr Diversity of bacterial communities in the plasmodia of myxomycetes
title_full_unstemmed Diversity of bacterial communities in the plasmodia of myxomycetes
title_short Diversity of bacterial communities in the plasmodia of myxomycetes
title_sort diversity of bacterial communities in the plasmodia of myxomycetes
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9773492/
https://www.ncbi.nlm.nih.gov/pubmed/36544088
http://dx.doi.org/10.1186/s12866-022-02725-5
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