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Site-Directed Spin Labeling EPR Spectroscopy for Determination of Albumin Structural Damage and Hypoalbuminemia in Critical COVID-19

The main factors in the COVID-19 pathology, which can initiate extensive structural changes at the cellular and molecular levels, are the generation of free radicals in abnormal amounts, and oxidative stress. Under “oxidative shock” conditions, the proteins undergo various modifications that affect...

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Autores principales: Georgieva, Ekaterina, Karamalakova, Yanka, Arabadzhiev, Georgi, Atanasov, Vasil, Kostandieva, Rositsa, Mitev, Mitko, Tsoneva, Vanya, Yovchev, Yovcho, Nikolova, Galina
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9774111/
https://www.ncbi.nlm.nih.gov/pubmed/36552520
http://dx.doi.org/10.3390/antiox11122311
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author Georgieva, Ekaterina
Karamalakova, Yanka
Arabadzhiev, Georgi
Atanasov, Vasil
Kostandieva, Rositsa
Mitev, Mitko
Tsoneva, Vanya
Yovchev, Yovcho
Nikolova, Galina
author_facet Georgieva, Ekaterina
Karamalakova, Yanka
Arabadzhiev, Georgi
Atanasov, Vasil
Kostandieva, Rositsa
Mitev, Mitko
Tsoneva, Vanya
Yovchev, Yovcho
Nikolova, Galina
author_sort Georgieva, Ekaterina
collection PubMed
description The main factors in the COVID-19 pathology, which can initiate extensive structural changes at the cellular and molecular levels, are the generation of free radicals in abnormal amounts, and oxidative stress. Under “oxidative shock” conditions, the proteins undergo various modifications that affect their function and activity, and as a result distribute malfunctioning protein derivatives in the body. Human serum albumin is a small globular protein characterized by a high overall binding capacity for neutral lipophilic and acidic dosage forms. The albumin concentration is crucial for the maintenance of plasma oncotic pressure, the transport of nutrients, amino acids, and drugs, the effectiveness of drug therapy, and the prevention of drug toxicity. Hypoalbuminemia and structural defects molecule in the protein suggest a risk of changed metabolism and increased plasma concentration of unbound drugs. Therefore, the albumin structural and functional changes accompanied by low protein levels can be a serious prerequisite for ineffective therapy, frequent complications, and high mortality in patients with SARS-CoV-2 infection. The current opinion aims the research community the application of Site-Directed Spin Labeling Electron Paramagnetic Resonance spectroscopy (SDSL-EPR) and 3-Maleimido-PROXYL radical in determining abnormalities of the albumin dynamics and protein concentrations in COVID-19 critical patients.
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spelling pubmed-97741112022-12-23 Site-Directed Spin Labeling EPR Spectroscopy for Determination of Albumin Structural Damage and Hypoalbuminemia in Critical COVID-19 Georgieva, Ekaterina Karamalakova, Yanka Arabadzhiev, Georgi Atanasov, Vasil Kostandieva, Rositsa Mitev, Mitko Tsoneva, Vanya Yovchev, Yovcho Nikolova, Galina Antioxidants (Basel) Opinion The main factors in the COVID-19 pathology, which can initiate extensive structural changes at the cellular and molecular levels, are the generation of free radicals in abnormal amounts, and oxidative stress. Under “oxidative shock” conditions, the proteins undergo various modifications that affect their function and activity, and as a result distribute malfunctioning protein derivatives in the body. Human serum albumin is a small globular protein characterized by a high overall binding capacity for neutral lipophilic and acidic dosage forms. The albumin concentration is crucial for the maintenance of plasma oncotic pressure, the transport of nutrients, amino acids, and drugs, the effectiveness of drug therapy, and the prevention of drug toxicity. Hypoalbuminemia and structural defects molecule in the protein suggest a risk of changed metabolism and increased plasma concentration of unbound drugs. Therefore, the albumin structural and functional changes accompanied by low protein levels can be a serious prerequisite for ineffective therapy, frequent complications, and high mortality in patients with SARS-CoV-2 infection. The current opinion aims the research community the application of Site-Directed Spin Labeling Electron Paramagnetic Resonance spectroscopy (SDSL-EPR) and 3-Maleimido-PROXYL radical in determining abnormalities of the albumin dynamics and protein concentrations in COVID-19 critical patients. MDPI 2022-11-22 /pmc/articles/PMC9774111/ /pubmed/36552520 http://dx.doi.org/10.3390/antiox11122311 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Opinion
Georgieva, Ekaterina
Karamalakova, Yanka
Arabadzhiev, Georgi
Atanasov, Vasil
Kostandieva, Rositsa
Mitev, Mitko
Tsoneva, Vanya
Yovchev, Yovcho
Nikolova, Galina
Site-Directed Spin Labeling EPR Spectroscopy for Determination of Albumin Structural Damage and Hypoalbuminemia in Critical COVID-19
title Site-Directed Spin Labeling EPR Spectroscopy for Determination of Albumin Structural Damage and Hypoalbuminemia in Critical COVID-19
title_full Site-Directed Spin Labeling EPR Spectroscopy for Determination of Albumin Structural Damage and Hypoalbuminemia in Critical COVID-19
title_fullStr Site-Directed Spin Labeling EPR Spectroscopy for Determination of Albumin Structural Damage and Hypoalbuminemia in Critical COVID-19
title_full_unstemmed Site-Directed Spin Labeling EPR Spectroscopy for Determination of Albumin Structural Damage and Hypoalbuminemia in Critical COVID-19
title_short Site-Directed Spin Labeling EPR Spectroscopy for Determination of Albumin Structural Damage and Hypoalbuminemia in Critical COVID-19
title_sort site-directed spin labeling epr spectroscopy for determination of albumin structural damage and hypoalbuminemia in critical covid-19
topic Opinion
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9774111/
https://www.ncbi.nlm.nih.gov/pubmed/36552520
http://dx.doi.org/10.3390/antiox11122311
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