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Antioxidant and Chemopreventive Activity of Protein Hydrolysates from Raw and Germinated Flour of Legumes with Commercial Interest in Colorectal Cancer
Legumes are a highly nutritious source of plant protein, fiber, minerals and vitamins. However, they also contain several bioactive compounds with significant potential benefits for human health. The objectives of this study were to evaluate the antioxidant, antitumor and chemopreventive activity of...
Autores principales: | , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9774143/ https://www.ncbi.nlm.nih.gov/pubmed/36552629 http://dx.doi.org/10.3390/antiox11122421 |
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author | Fuel, Marco Mesas, Cristina Martínez, Rosario Ortiz, Raúl Quiñonero, Francisco Bermúdez, Francisco Gutiérrez, Natalia Torres, Ana M. Kapravelou, Garyfallia Lozano, Aída Perazzoli, Gloria Prados, Jose Porres, Jesús M. Melguizo, Consolación |
author_facet | Fuel, Marco Mesas, Cristina Martínez, Rosario Ortiz, Raúl Quiñonero, Francisco Bermúdez, Francisco Gutiérrez, Natalia Torres, Ana M. Kapravelou, Garyfallia Lozano, Aída Perazzoli, Gloria Prados, Jose Porres, Jesús M. Melguizo, Consolación |
author_sort | Fuel, Marco |
collection | PubMed |
description | Legumes are a highly nutritious source of plant protein, fiber, minerals and vitamins. However, they also contain several bioactive compounds with significant potential benefits for human health. The objectives of this study were to evaluate the antioxidant, antitumor and chemopreventive activity of functional extracts from legumes using raw and germinated flours of six legume species of commercial interest. The methodology carried out consisted on the development of protein hydrolysates, assessment of their antioxidant capacity and in vitro tests on T84, HCT15 and SW480 colorectal cancer (CRC) cell lines. Our results showed a high antitumor activity of protein hydrolysate from M. sativa. Likewise, when combined with 5-Fluorouracile (5-Fu), there was a synergistic effect using extract concentrations from 50 to 175 µg/mL and 5-Fu concentrations from 1.5 to 5 µM. Similarly, the induction effect on detoxifying enzymes by the extracts of M. sativa, germinated V. faba Baraca × LVzt1 and V. narbonensis, which produced a higher induction rate than the positive control sulforaphane (10 µM), should be highlighted. Therefore, incorporating these enzymes into the diet could provide nutritional effects, as well as play an effective role in cancer chemoprevention and therapy. |
format | Online Article Text |
id | pubmed-9774143 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-97741432022-12-23 Antioxidant and Chemopreventive Activity of Protein Hydrolysates from Raw and Germinated Flour of Legumes with Commercial Interest in Colorectal Cancer Fuel, Marco Mesas, Cristina Martínez, Rosario Ortiz, Raúl Quiñonero, Francisco Bermúdez, Francisco Gutiérrez, Natalia Torres, Ana M. Kapravelou, Garyfallia Lozano, Aída Perazzoli, Gloria Prados, Jose Porres, Jesús M. Melguizo, Consolación Antioxidants (Basel) Article Legumes are a highly nutritious source of plant protein, fiber, minerals and vitamins. However, they also contain several bioactive compounds with significant potential benefits for human health. The objectives of this study were to evaluate the antioxidant, antitumor and chemopreventive activity of functional extracts from legumes using raw and germinated flours of six legume species of commercial interest. The methodology carried out consisted on the development of protein hydrolysates, assessment of their antioxidant capacity and in vitro tests on T84, HCT15 and SW480 colorectal cancer (CRC) cell lines. Our results showed a high antitumor activity of protein hydrolysate from M. sativa. Likewise, when combined with 5-Fluorouracile (5-Fu), there was a synergistic effect using extract concentrations from 50 to 175 µg/mL and 5-Fu concentrations from 1.5 to 5 µM. Similarly, the induction effect on detoxifying enzymes by the extracts of M. sativa, germinated V. faba Baraca × LVzt1 and V. narbonensis, which produced a higher induction rate than the positive control sulforaphane (10 µM), should be highlighted. Therefore, incorporating these enzymes into the diet could provide nutritional effects, as well as play an effective role in cancer chemoprevention and therapy. MDPI 2022-12-08 /pmc/articles/PMC9774143/ /pubmed/36552629 http://dx.doi.org/10.3390/antiox11122421 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Fuel, Marco Mesas, Cristina Martínez, Rosario Ortiz, Raúl Quiñonero, Francisco Bermúdez, Francisco Gutiérrez, Natalia Torres, Ana M. Kapravelou, Garyfallia Lozano, Aída Perazzoli, Gloria Prados, Jose Porres, Jesús M. Melguizo, Consolación Antioxidant and Chemopreventive Activity of Protein Hydrolysates from Raw and Germinated Flour of Legumes with Commercial Interest in Colorectal Cancer |
title | Antioxidant and Chemopreventive Activity of Protein Hydrolysates from Raw and Germinated Flour of Legumes with Commercial Interest in Colorectal Cancer |
title_full | Antioxidant and Chemopreventive Activity of Protein Hydrolysates from Raw and Germinated Flour of Legumes with Commercial Interest in Colorectal Cancer |
title_fullStr | Antioxidant and Chemopreventive Activity of Protein Hydrolysates from Raw and Germinated Flour of Legumes with Commercial Interest in Colorectal Cancer |
title_full_unstemmed | Antioxidant and Chemopreventive Activity of Protein Hydrolysates from Raw and Germinated Flour of Legumes with Commercial Interest in Colorectal Cancer |
title_short | Antioxidant and Chemopreventive Activity of Protein Hydrolysates from Raw and Germinated Flour of Legumes with Commercial Interest in Colorectal Cancer |
title_sort | antioxidant and chemopreventive activity of protein hydrolysates from raw and germinated flour of legumes with commercial interest in colorectal cancer |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9774143/ https://www.ncbi.nlm.nih.gov/pubmed/36552629 http://dx.doi.org/10.3390/antiox11122421 |
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