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Antioxidant and Chemopreventive Activity of Protein Hydrolysates from Raw and Germinated Flour of Legumes with Commercial Interest in Colorectal Cancer

Legumes are a highly nutritious source of plant protein, fiber, minerals and vitamins. However, they also contain several bioactive compounds with significant potential benefits for human health. The objectives of this study were to evaluate the antioxidant, antitumor and chemopreventive activity of...

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Autores principales: Fuel, Marco, Mesas, Cristina, Martínez, Rosario, Ortiz, Raúl, Quiñonero, Francisco, Bermúdez, Francisco, Gutiérrez, Natalia, Torres, Ana M., Kapravelou, Garyfallia, Lozano, Aída, Perazzoli, Gloria, Prados, Jose, Porres, Jesús M., Melguizo, Consolación
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9774143/
https://www.ncbi.nlm.nih.gov/pubmed/36552629
http://dx.doi.org/10.3390/antiox11122421
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author Fuel, Marco
Mesas, Cristina
Martínez, Rosario
Ortiz, Raúl
Quiñonero, Francisco
Bermúdez, Francisco
Gutiérrez, Natalia
Torres, Ana M.
Kapravelou, Garyfallia
Lozano, Aída
Perazzoli, Gloria
Prados, Jose
Porres, Jesús M.
Melguizo, Consolación
author_facet Fuel, Marco
Mesas, Cristina
Martínez, Rosario
Ortiz, Raúl
Quiñonero, Francisco
Bermúdez, Francisco
Gutiérrez, Natalia
Torres, Ana M.
Kapravelou, Garyfallia
Lozano, Aída
Perazzoli, Gloria
Prados, Jose
Porres, Jesús M.
Melguizo, Consolación
author_sort Fuel, Marco
collection PubMed
description Legumes are a highly nutritious source of plant protein, fiber, minerals and vitamins. However, they also contain several bioactive compounds with significant potential benefits for human health. The objectives of this study were to evaluate the antioxidant, antitumor and chemopreventive activity of functional extracts from legumes using raw and germinated flours of six legume species of commercial interest. The methodology carried out consisted on the development of protein hydrolysates, assessment of their antioxidant capacity and in vitro tests on T84, HCT15 and SW480 colorectal cancer (CRC) cell lines. Our results showed a high antitumor activity of protein hydrolysate from M. sativa. Likewise, when combined with 5-Fluorouracile (5-Fu), there was a synergistic effect using extract concentrations from 50 to 175 µg/mL and 5-Fu concentrations from 1.5 to 5 µM. Similarly, the induction effect on detoxifying enzymes by the extracts of M. sativa, germinated V. faba Baraca × LVzt1 and V. narbonensis, which produced a higher induction rate than the positive control sulforaphane (10 µM), should be highlighted. Therefore, incorporating these enzymes into the diet could provide nutritional effects, as well as play an effective role in cancer chemoprevention and therapy.
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spelling pubmed-97741432022-12-23 Antioxidant and Chemopreventive Activity of Protein Hydrolysates from Raw and Germinated Flour of Legumes with Commercial Interest in Colorectal Cancer Fuel, Marco Mesas, Cristina Martínez, Rosario Ortiz, Raúl Quiñonero, Francisco Bermúdez, Francisco Gutiérrez, Natalia Torres, Ana M. Kapravelou, Garyfallia Lozano, Aída Perazzoli, Gloria Prados, Jose Porres, Jesús M. Melguizo, Consolación Antioxidants (Basel) Article Legumes are a highly nutritious source of plant protein, fiber, minerals and vitamins. However, they also contain several bioactive compounds with significant potential benefits for human health. The objectives of this study were to evaluate the antioxidant, antitumor and chemopreventive activity of functional extracts from legumes using raw and germinated flours of six legume species of commercial interest. The methodology carried out consisted on the development of protein hydrolysates, assessment of their antioxidant capacity and in vitro tests on T84, HCT15 and SW480 colorectal cancer (CRC) cell lines. Our results showed a high antitumor activity of protein hydrolysate from M. sativa. Likewise, when combined with 5-Fluorouracile (5-Fu), there was a synergistic effect using extract concentrations from 50 to 175 µg/mL and 5-Fu concentrations from 1.5 to 5 µM. Similarly, the induction effect on detoxifying enzymes by the extracts of M. sativa, germinated V. faba Baraca × LVzt1 and V. narbonensis, which produced a higher induction rate than the positive control sulforaphane (10 µM), should be highlighted. Therefore, incorporating these enzymes into the diet could provide nutritional effects, as well as play an effective role in cancer chemoprevention and therapy. MDPI 2022-12-08 /pmc/articles/PMC9774143/ /pubmed/36552629 http://dx.doi.org/10.3390/antiox11122421 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Fuel, Marco
Mesas, Cristina
Martínez, Rosario
Ortiz, Raúl
Quiñonero, Francisco
Bermúdez, Francisco
Gutiérrez, Natalia
Torres, Ana M.
Kapravelou, Garyfallia
Lozano, Aída
Perazzoli, Gloria
Prados, Jose
Porres, Jesús M.
Melguizo, Consolación
Antioxidant and Chemopreventive Activity of Protein Hydrolysates from Raw and Germinated Flour of Legumes with Commercial Interest in Colorectal Cancer
title Antioxidant and Chemopreventive Activity of Protein Hydrolysates from Raw and Germinated Flour of Legumes with Commercial Interest in Colorectal Cancer
title_full Antioxidant and Chemopreventive Activity of Protein Hydrolysates from Raw and Germinated Flour of Legumes with Commercial Interest in Colorectal Cancer
title_fullStr Antioxidant and Chemopreventive Activity of Protein Hydrolysates from Raw and Germinated Flour of Legumes with Commercial Interest in Colorectal Cancer
title_full_unstemmed Antioxidant and Chemopreventive Activity of Protein Hydrolysates from Raw and Germinated Flour of Legumes with Commercial Interest in Colorectal Cancer
title_short Antioxidant and Chemopreventive Activity of Protein Hydrolysates from Raw and Germinated Flour of Legumes with Commercial Interest in Colorectal Cancer
title_sort antioxidant and chemopreventive activity of protein hydrolysates from raw and germinated flour of legumes with commercial interest in colorectal cancer
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9774143/
https://www.ncbi.nlm.nih.gov/pubmed/36552629
http://dx.doi.org/10.3390/antiox11122421
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