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Maqui Berry and Ginseng Extracts Reduce Cigarette Smoke-Induced Cell Injury in a 3D Bone Co-Culture Model
Cigarette smoking-induced oxidative stress has harmful effects on bone metabolism. Maqui berry extract (MBE) and ginseng extract (GE) are two naturally occurring antioxidants that have been shown to reduce oxidative stress. By using an osteoblast and osteoclast three-dimensional co-culture system, w...
Autores principales: | , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9774157/ https://www.ncbi.nlm.nih.gov/pubmed/36552669 http://dx.doi.org/10.3390/antiox11122460 |
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author | Guo, Huizhi Weng, Weidong Zhang, Shuncong Rinderknecht, Helen Braun, Bianca Breinbauer, Regina Gupta, Purva Kumar, Ashok Ehnert, Sabrina Histing, Tina Nussler, Andreas K. Aspera-Werz, Romina H. |
author_facet | Guo, Huizhi Weng, Weidong Zhang, Shuncong Rinderknecht, Helen Braun, Bianca Breinbauer, Regina Gupta, Purva Kumar, Ashok Ehnert, Sabrina Histing, Tina Nussler, Andreas K. Aspera-Werz, Romina H. |
author_sort | Guo, Huizhi |
collection | PubMed |
description | Cigarette smoking-induced oxidative stress has harmful effects on bone metabolism. Maqui berry extract (MBE) and ginseng extract (GE) are two naturally occurring antioxidants that have been shown to reduce oxidative stress. By using an osteoblast and osteoclast three-dimensional co-culture system, we investigated the effects of MBE and GE on bone cells exposed to cigarette smoke extract (CSE). The cell viability and function of the co-culture system were measured on day 14. Markers of bone cell differentiation and oxidative stress were evaluated at gene and protein levels on day 7. The results showed that exposure to CSE induced osteoporotic-like alterations in the co-culture system, while 1.5 µg/mL MBE and 50 µg/mL GE improved CSE-impaired osteoblast function and decreased CSE-induced osteoclast function. The molecular mechanism of MBE and GE in preventing CSE-induced bone cell damage is linked with the inhibition of the NF-κB signaling pathway and the activation of the Nrf2 signaling pathway. Therefore, MBE and GE can reduce CSE-induced detrimental effects on bone cells and, thus, prevent smoking-induced alterations in bone cell homeostasis. These two antioxidants are thus suitable supplements to support bone regeneration in smokers. |
format | Online Article Text |
id | pubmed-9774157 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-97741572022-12-23 Maqui Berry and Ginseng Extracts Reduce Cigarette Smoke-Induced Cell Injury in a 3D Bone Co-Culture Model Guo, Huizhi Weng, Weidong Zhang, Shuncong Rinderknecht, Helen Braun, Bianca Breinbauer, Regina Gupta, Purva Kumar, Ashok Ehnert, Sabrina Histing, Tina Nussler, Andreas K. Aspera-Werz, Romina H. Antioxidants (Basel) Article Cigarette smoking-induced oxidative stress has harmful effects on bone metabolism. Maqui berry extract (MBE) and ginseng extract (GE) are two naturally occurring antioxidants that have been shown to reduce oxidative stress. By using an osteoblast and osteoclast three-dimensional co-culture system, we investigated the effects of MBE and GE on bone cells exposed to cigarette smoke extract (CSE). The cell viability and function of the co-culture system were measured on day 14. Markers of bone cell differentiation and oxidative stress were evaluated at gene and protein levels on day 7. The results showed that exposure to CSE induced osteoporotic-like alterations in the co-culture system, while 1.5 µg/mL MBE and 50 µg/mL GE improved CSE-impaired osteoblast function and decreased CSE-induced osteoclast function. The molecular mechanism of MBE and GE in preventing CSE-induced bone cell damage is linked with the inhibition of the NF-κB signaling pathway and the activation of the Nrf2 signaling pathway. Therefore, MBE and GE can reduce CSE-induced detrimental effects on bone cells and, thus, prevent smoking-induced alterations in bone cell homeostasis. These two antioxidants are thus suitable supplements to support bone regeneration in smokers. MDPI 2022-12-14 /pmc/articles/PMC9774157/ /pubmed/36552669 http://dx.doi.org/10.3390/antiox11122460 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Guo, Huizhi Weng, Weidong Zhang, Shuncong Rinderknecht, Helen Braun, Bianca Breinbauer, Regina Gupta, Purva Kumar, Ashok Ehnert, Sabrina Histing, Tina Nussler, Andreas K. Aspera-Werz, Romina H. Maqui Berry and Ginseng Extracts Reduce Cigarette Smoke-Induced Cell Injury in a 3D Bone Co-Culture Model |
title | Maqui Berry and Ginseng Extracts Reduce Cigarette Smoke-Induced Cell Injury in a 3D Bone Co-Culture Model |
title_full | Maqui Berry and Ginseng Extracts Reduce Cigarette Smoke-Induced Cell Injury in a 3D Bone Co-Culture Model |
title_fullStr | Maqui Berry and Ginseng Extracts Reduce Cigarette Smoke-Induced Cell Injury in a 3D Bone Co-Culture Model |
title_full_unstemmed | Maqui Berry and Ginseng Extracts Reduce Cigarette Smoke-Induced Cell Injury in a 3D Bone Co-Culture Model |
title_short | Maqui Berry and Ginseng Extracts Reduce Cigarette Smoke-Induced Cell Injury in a 3D Bone Co-Culture Model |
title_sort | maqui berry and ginseng extracts reduce cigarette smoke-induced cell injury in a 3d bone co-culture model |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9774157/ https://www.ncbi.nlm.nih.gov/pubmed/36552669 http://dx.doi.org/10.3390/antiox11122460 |
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