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Three-Dimensional Bioprinting with Alginate by Freeform Reversible Embedding of Suspended Hydrogels with Tunable Physical Properties and Cell Proliferation

Extrusion-based three-dimensional (3D) bioprinting is an emerging technology that allows for rapid bio-fabrication of scaffolds with live cells. Alginate is a soft biomaterial that has been studied extensively as a bio-ink to support cell growth in 3D constructs. However, native alginate is a bio-in...

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Autores principales: Zhu, Yuanjia, Stark, Charles J., Madira, Sarah, Ethiraj, Sidarth, Venkatesh, Akshay, Anilkumar, Shreya, Jung, Jinsuh, Lee, Seunghyun, Wu, Catherine A., Walsh, Sabrina K., Stankovich, Gabriel A., Woo, Yi-Ping Joseph
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9774270/
https://www.ncbi.nlm.nih.gov/pubmed/36551013
http://dx.doi.org/10.3390/bioengineering9120807
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author Zhu, Yuanjia
Stark, Charles J.
Madira, Sarah
Ethiraj, Sidarth
Venkatesh, Akshay
Anilkumar, Shreya
Jung, Jinsuh
Lee, Seunghyun
Wu, Catherine A.
Walsh, Sabrina K.
Stankovich, Gabriel A.
Woo, Yi-Ping Joseph
author_facet Zhu, Yuanjia
Stark, Charles J.
Madira, Sarah
Ethiraj, Sidarth
Venkatesh, Akshay
Anilkumar, Shreya
Jung, Jinsuh
Lee, Seunghyun
Wu, Catherine A.
Walsh, Sabrina K.
Stankovich, Gabriel A.
Woo, Yi-Ping Joseph
author_sort Zhu, Yuanjia
collection PubMed
description Extrusion-based three-dimensional (3D) bioprinting is an emerging technology that allows for rapid bio-fabrication of scaffolds with live cells. Alginate is a soft biomaterial that has been studied extensively as a bio-ink to support cell growth in 3D constructs. However, native alginate is a bio-inert material that requires modifications to allow for cell adhesion and cell growth. Cells grown in modified alginates with the RGD (arginine-glycine-aspartate) motif, a naturally existing tripeptide sequence that is crucial to cell adhesion and proliferation, demonstrate enhanced cell adhesion, spreading, and differentiation. Recently, the bioprinting technique using freeform reversible embedding of suspended hydrogels (FRESH) has revolutionized 3D bioprinting, enabling the use of soft bio-inks that would otherwise collapse in air. However, the printability of RGD-modified alginates using the FRESH technique has not been evaluated. The associated physical properties and bioactivity of 3D bio-printed alginates after RGD modification remains unclear. In this study, we characterized the physical properties, printability, and cellular proliferation of native and RGD-modified alginate after extrusion-based 3D bioprinting in FRESH. We demonstrated tunable physical properties of native and RGD-modified alginates after FRESH 3D bioprinting. Sodium alginate with RGD modification, especially at a high concentration, was associated with greatly improved cell viability and integrin clustering, which further enhanced cell proliferation.
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spelling pubmed-97742702022-12-23 Three-Dimensional Bioprinting with Alginate by Freeform Reversible Embedding of Suspended Hydrogels with Tunable Physical Properties and Cell Proliferation Zhu, Yuanjia Stark, Charles J. Madira, Sarah Ethiraj, Sidarth Venkatesh, Akshay Anilkumar, Shreya Jung, Jinsuh Lee, Seunghyun Wu, Catherine A. Walsh, Sabrina K. Stankovich, Gabriel A. Woo, Yi-Ping Joseph Bioengineering (Basel) Article Extrusion-based three-dimensional (3D) bioprinting is an emerging technology that allows for rapid bio-fabrication of scaffolds with live cells. Alginate is a soft biomaterial that has been studied extensively as a bio-ink to support cell growth in 3D constructs. However, native alginate is a bio-inert material that requires modifications to allow for cell adhesion and cell growth. Cells grown in modified alginates with the RGD (arginine-glycine-aspartate) motif, a naturally existing tripeptide sequence that is crucial to cell adhesion and proliferation, demonstrate enhanced cell adhesion, spreading, and differentiation. Recently, the bioprinting technique using freeform reversible embedding of suspended hydrogels (FRESH) has revolutionized 3D bioprinting, enabling the use of soft bio-inks that would otherwise collapse in air. However, the printability of RGD-modified alginates using the FRESH technique has not been evaluated. The associated physical properties and bioactivity of 3D bio-printed alginates after RGD modification remains unclear. In this study, we characterized the physical properties, printability, and cellular proliferation of native and RGD-modified alginate after extrusion-based 3D bioprinting in FRESH. We demonstrated tunable physical properties of native and RGD-modified alginates after FRESH 3D bioprinting. Sodium alginate with RGD modification, especially at a high concentration, was associated with greatly improved cell viability and integrin clustering, which further enhanced cell proliferation. MDPI 2022-12-15 /pmc/articles/PMC9774270/ /pubmed/36551013 http://dx.doi.org/10.3390/bioengineering9120807 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Zhu, Yuanjia
Stark, Charles J.
Madira, Sarah
Ethiraj, Sidarth
Venkatesh, Akshay
Anilkumar, Shreya
Jung, Jinsuh
Lee, Seunghyun
Wu, Catherine A.
Walsh, Sabrina K.
Stankovich, Gabriel A.
Woo, Yi-Ping Joseph
Three-Dimensional Bioprinting with Alginate by Freeform Reversible Embedding of Suspended Hydrogels with Tunable Physical Properties and Cell Proliferation
title Three-Dimensional Bioprinting with Alginate by Freeform Reversible Embedding of Suspended Hydrogels with Tunable Physical Properties and Cell Proliferation
title_full Three-Dimensional Bioprinting with Alginate by Freeform Reversible Embedding of Suspended Hydrogels with Tunable Physical Properties and Cell Proliferation
title_fullStr Three-Dimensional Bioprinting with Alginate by Freeform Reversible Embedding of Suspended Hydrogels with Tunable Physical Properties and Cell Proliferation
title_full_unstemmed Three-Dimensional Bioprinting with Alginate by Freeform Reversible Embedding of Suspended Hydrogels with Tunable Physical Properties and Cell Proliferation
title_short Three-Dimensional Bioprinting with Alginate by Freeform Reversible Embedding of Suspended Hydrogels with Tunable Physical Properties and Cell Proliferation
title_sort three-dimensional bioprinting with alginate by freeform reversible embedding of suspended hydrogels with tunable physical properties and cell proliferation
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9774270/
https://www.ncbi.nlm.nih.gov/pubmed/36551013
http://dx.doi.org/10.3390/bioengineering9120807
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