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Genotypic Distribution and the Epidemiology of Multidrug Resistant Tuberculosis in Upper Northern Thailand

The epidemiology and genotypes of multidrug-resistant tuberculosis (MDR-TB), a global public health threat, remain limited. The genotypic distribution and factors associated with MDR-TB in upper northern Thailand between 2015 and 2019 were investigated. The DNA sequencing of rpoB, katG, and inhA pro...

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Autores principales: Saikaew, Sukanya, Thongprachum, Aksara, Pongsararuk, Rodjana, Thanraka, Aungkana, Kunyanone, Naowarat, Chaiyasirinroje, Boonchai, Luangsook, Praphan, Butr-Indr, Bordin, Phunpae, Ponrut, Wattananandkul, Usanee
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9774302/
https://www.ncbi.nlm.nih.gov/pubmed/36551389
http://dx.doi.org/10.3390/antibiotics11121733
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author Saikaew, Sukanya
Thongprachum, Aksara
Pongsararuk, Rodjana
Thanraka, Aungkana
Kunyanone, Naowarat
Chaiyasirinroje, Boonchai
Luangsook, Praphan
Butr-Indr, Bordin
Phunpae, Ponrut
Wattananandkul, Usanee
author_facet Saikaew, Sukanya
Thongprachum, Aksara
Pongsararuk, Rodjana
Thanraka, Aungkana
Kunyanone, Naowarat
Chaiyasirinroje, Boonchai
Luangsook, Praphan
Butr-Indr, Bordin
Phunpae, Ponrut
Wattananandkul, Usanee
author_sort Saikaew, Sukanya
collection PubMed
description The epidemiology and genotypes of multidrug-resistant tuberculosis (MDR-TB), a global public health threat, remain limited. The genotypic distribution and factors associated with MDR-TB in upper northern Thailand between 2015 and 2019 were investigated. The DNA sequencing of rpoB, katG, and inhA promoter of 51 multidrug-resistant Mycobacterium tuberculosis isolates revealed nine patterns of the rpoB gene mutation distributed in seven provinces. The S531L mutation was the most common mutation in all provinces. The rpoB mutation in Chiang Rai, Chiang Mai, and Lampang was highly diverse compared to other areas. Here, the mutation profiles that have yet to be reported in northern Thailand (H526P, Q513P, and H526C) were detected in Chiang Rai province. The S315T katG mutation was the most common genotype associated with INH resistance, especially in Chiang Mai and Lampang. Further analysis of data from 110 TB patients (42 MDR-TB and 68 drug-susceptible TB) revealed that <60 years of age was a significant factor associated with MDR-TB (OR = 0.316, 95% CI 0.128–0.784, p = 0.011) and ≥60 years of age was a significant factor associated with the S315T katG-mutation (OR = 8.867, 95% CI 0.981–80.177, p = 0.047). This study highlighted the necessity for continuous surveillance and risk factor monitoring for effective control of MDR-TB.
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spelling pubmed-97743022022-12-23 Genotypic Distribution and the Epidemiology of Multidrug Resistant Tuberculosis in Upper Northern Thailand Saikaew, Sukanya Thongprachum, Aksara Pongsararuk, Rodjana Thanraka, Aungkana Kunyanone, Naowarat Chaiyasirinroje, Boonchai Luangsook, Praphan Butr-Indr, Bordin Phunpae, Ponrut Wattananandkul, Usanee Antibiotics (Basel) Article The epidemiology and genotypes of multidrug-resistant tuberculosis (MDR-TB), a global public health threat, remain limited. The genotypic distribution and factors associated with MDR-TB in upper northern Thailand between 2015 and 2019 were investigated. The DNA sequencing of rpoB, katG, and inhA promoter of 51 multidrug-resistant Mycobacterium tuberculosis isolates revealed nine patterns of the rpoB gene mutation distributed in seven provinces. The S531L mutation was the most common mutation in all provinces. The rpoB mutation in Chiang Rai, Chiang Mai, and Lampang was highly diverse compared to other areas. Here, the mutation profiles that have yet to be reported in northern Thailand (H526P, Q513P, and H526C) were detected in Chiang Rai province. The S315T katG mutation was the most common genotype associated with INH resistance, especially in Chiang Mai and Lampang. Further analysis of data from 110 TB patients (42 MDR-TB and 68 drug-susceptible TB) revealed that <60 years of age was a significant factor associated with MDR-TB (OR = 0.316, 95% CI 0.128–0.784, p = 0.011) and ≥60 years of age was a significant factor associated with the S315T katG-mutation (OR = 8.867, 95% CI 0.981–80.177, p = 0.047). This study highlighted the necessity for continuous surveillance and risk factor monitoring for effective control of MDR-TB. MDPI 2022-12-01 /pmc/articles/PMC9774302/ /pubmed/36551389 http://dx.doi.org/10.3390/antibiotics11121733 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Saikaew, Sukanya
Thongprachum, Aksara
Pongsararuk, Rodjana
Thanraka, Aungkana
Kunyanone, Naowarat
Chaiyasirinroje, Boonchai
Luangsook, Praphan
Butr-Indr, Bordin
Phunpae, Ponrut
Wattananandkul, Usanee
Genotypic Distribution and the Epidemiology of Multidrug Resistant Tuberculosis in Upper Northern Thailand
title Genotypic Distribution and the Epidemiology of Multidrug Resistant Tuberculosis in Upper Northern Thailand
title_full Genotypic Distribution and the Epidemiology of Multidrug Resistant Tuberculosis in Upper Northern Thailand
title_fullStr Genotypic Distribution and the Epidemiology of Multidrug Resistant Tuberculosis in Upper Northern Thailand
title_full_unstemmed Genotypic Distribution and the Epidemiology of Multidrug Resistant Tuberculosis in Upper Northern Thailand
title_short Genotypic Distribution and the Epidemiology of Multidrug Resistant Tuberculosis in Upper Northern Thailand
title_sort genotypic distribution and the epidemiology of multidrug resistant tuberculosis in upper northern thailand
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9774302/
https://www.ncbi.nlm.nih.gov/pubmed/36551389
http://dx.doi.org/10.3390/antibiotics11121733
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