Genome-Wide Identification and Phylogenetic Analysis of TRP Gene Family Members in Saurian

SIMPLE SUMMARY: A total of 251 putative TRPs from saurian are divided into 2 groups, belonging to 6 TRPs subfamilies, excluding the TRPN subfamily. The most conserved proteins of TRP box 1 are located in motif 1, and those of TRP box 2 are located in motif 10. The TRPA and TRPV in saurian tend to be one cluster, as a sister cluster with TRPC, and the TRPM is a root of group I. TRPM, TRPV, and TRPP are clustered into two clades, and TRPP is organized into TRP PKD1-like and PKD2-like. Segmental duplications mainly occur in the TRPM subfamily, and the tandem duplications only occur in the TRPV subfamily. Fifteen sites were under positive selection for TRPA1 and TRPV2 genes. The branch model revealed that positive selection fit the data better than the null model for the genes TRPC5 and TRPV3. ABSTRACT: The transient receptor potential plays a critical role in the sensory nervous systems of vertebrates in response to various mechanisms and stimuli, such as environmental temperature. We studied the physiological adaptive evolution of the TRP gene in the saurian family and performed a comprehensive analysis to identify the evolution of the thermo-TRPs channels. All 251 putative TRPs were divided into 6 subfamilies, except TRPN, from the 8 saurian genomes. Multiple characteristics of these genes were analyzed. The results showed that the most conserved proteins of TRP box 1 were located in motif 1, and those of TRP box 2 were located in motif 10. The TRPA and TRPV in saurian tend to be one cluster, as a sister cluster with TRPC, and the TRPM is the root of group I. The TRPM, TRPV, and TRPP were clustered into two clades, and TRPP were organized into TRP PKD1-like and PKD2-like. Segmental duplications mainly occurred in the TRPM subfamily, and tandem duplications only occurred in the TRPV subfamily. There were 15 sites to be under positive selection for TRPA1 and TRPV2 genes. In summary, gene structure, chromosomal location, gene duplication, synteny analysis, and selective pressure at the molecular level provided some new evidence for genetic adaptation to the environment. This result provides a basis for identifying and classifying TRP genes and contributes to further elucidating their potential function in thermal sensors.