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New Insights into the Cellular Toxicity of Carbon Quantum Dots to Escherichia coli

In this study, the cytotoxicity and toxic mechanism of carbon quantum dots (CQDs) to E. coli were evaluated in vitro. The synthetic CQDs were extremely small in size (~2.08 nm) and displayed strong fluorescence. The results demonstrated that CQDs showed good biocompatibility with E. coli within a sh...

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Autores principales: Qiang, Shirong, Zhang, Li, Li, Zhengbin, Liang, Jianjun, Li, Ping, Song, Jiayu, Guo, Kunling, Wang, Zihuan, Fan, Qiaohui
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9774514/
https://www.ncbi.nlm.nih.gov/pubmed/36552683
http://dx.doi.org/10.3390/antiox11122475
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author Qiang, Shirong
Zhang, Li
Li, Zhengbin
Liang, Jianjun
Li, Ping
Song, Jiayu
Guo, Kunling
Wang, Zihuan
Fan, Qiaohui
author_facet Qiang, Shirong
Zhang, Li
Li, Zhengbin
Liang, Jianjun
Li, Ping
Song, Jiayu
Guo, Kunling
Wang, Zihuan
Fan, Qiaohui
author_sort Qiang, Shirong
collection PubMed
description In this study, the cytotoxicity and toxic mechanism of carbon quantum dots (CQDs) to E. coli were evaluated in vitro. The synthetic CQDs were extremely small in size (~2.08 nm) and displayed strong fluorescence. The results demonstrated that CQDs showed good biocompatibility with E. coli within a short culture time. However, when the exposure time exceeded 24 h, the toxicity of CQDs became apparent, and the contents of reactive oxygen species, lactate dehydrogenase, and the crystal violet absorption rate increased significantly. To further explore the cytotoxic mechanism, approaches including confocal laser scanning microscopy, scanning electron microscopy, and biological transmission electron microscopy combined with zeta potential tests, osmotic pressure measurement, and comet assays were performed. On the one hand, the CQDs altered the surface charges of cells and induced lipid peroxidation by adhesion on the surface of E. coli, leading to an increase in the permeability of the cell wall. On the other hand, when the concentration of CQDs reached 200 µg/mL, the osmotic pressure of the extracellular environment was significantly reduced. These are the main factors that lead to cell edema and death. Finally, the comet assays confirmed that CQDs could induce DNA damage, which could inhibit the proliferation of E. coli.
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spelling pubmed-97745142022-12-23 New Insights into the Cellular Toxicity of Carbon Quantum Dots to Escherichia coli Qiang, Shirong Zhang, Li Li, Zhengbin Liang, Jianjun Li, Ping Song, Jiayu Guo, Kunling Wang, Zihuan Fan, Qiaohui Antioxidants (Basel) Article In this study, the cytotoxicity and toxic mechanism of carbon quantum dots (CQDs) to E. coli were evaluated in vitro. The synthetic CQDs were extremely small in size (~2.08 nm) and displayed strong fluorescence. The results demonstrated that CQDs showed good biocompatibility with E. coli within a short culture time. However, when the exposure time exceeded 24 h, the toxicity of CQDs became apparent, and the contents of reactive oxygen species, lactate dehydrogenase, and the crystal violet absorption rate increased significantly. To further explore the cytotoxic mechanism, approaches including confocal laser scanning microscopy, scanning electron microscopy, and biological transmission electron microscopy combined with zeta potential tests, osmotic pressure measurement, and comet assays were performed. On the one hand, the CQDs altered the surface charges of cells and induced lipid peroxidation by adhesion on the surface of E. coli, leading to an increase in the permeability of the cell wall. On the other hand, when the concentration of CQDs reached 200 µg/mL, the osmotic pressure of the extracellular environment was significantly reduced. These are the main factors that lead to cell edema and death. Finally, the comet assays confirmed that CQDs could induce DNA damage, which could inhibit the proliferation of E. coli. MDPI 2022-12-16 /pmc/articles/PMC9774514/ /pubmed/36552683 http://dx.doi.org/10.3390/antiox11122475 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Qiang, Shirong
Zhang, Li
Li, Zhengbin
Liang, Jianjun
Li, Ping
Song, Jiayu
Guo, Kunling
Wang, Zihuan
Fan, Qiaohui
New Insights into the Cellular Toxicity of Carbon Quantum Dots to Escherichia coli
title New Insights into the Cellular Toxicity of Carbon Quantum Dots to Escherichia coli
title_full New Insights into the Cellular Toxicity of Carbon Quantum Dots to Escherichia coli
title_fullStr New Insights into the Cellular Toxicity of Carbon Quantum Dots to Escherichia coli
title_full_unstemmed New Insights into the Cellular Toxicity of Carbon Quantum Dots to Escherichia coli
title_short New Insights into the Cellular Toxicity of Carbon Quantum Dots to Escherichia coli
title_sort new insights into the cellular toxicity of carbon quantum dots to escherichia coli
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9774514/
https://www.ncbi.nlm.nih.gov/pubmed/36552683
http://dx.doi.org/10.3390/antiox11122475
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