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Emergence of Colistin-Resistant Acinetobacter junii in China

The increasing number of multidrug-resistant Gram-negative bacteria presents a serious threat to global health. However, colistin-resistant Acinetobacter junii has rarely been reported. We identified a colistin-resistant A. junii clinical isolate, AJ6079, in blood. The colony of AJ6079 presented a d...

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Autores principales: Wang, Zhiren, Fan, Xuanyang, Wang, Shuyi, Li, Shuguang, Gao, Yue, Wang, Hui, Li, Henan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9774529/
https://www.ncbi.nlm.nih.gov/pubmed/36551350
http://dx.doi.org/10.3390/antibiotics11121693
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author Wang, Zhiren
Fan, Xuanyang
Wang, Shuyi
Li, Shuguang
Gao, Yue
Wang, Hui
Li, Henan
author_facet Wang, Zhiren
Fan, Xuanyang
Wang, Shuyi
Li, Shuguang
Gao, Yue
Wang, Hui
Li, Henan
author_sort Wang, Zhiren
collection PubMed
description The increasing number of multidrug-resistant Gram-negative bacteria presents a serious threat to global health. However, colistin-resistant Acinetobacter junii has rarely been reported. We identified a colistin-resistant A. junii clinical isolate, AJ6079, in blood. The colony of AJ6079 presented a dry phenotype, and it was difficult to form a bacterial suspension, whilst transmission electron microscopy revealed that AJ6079 possessed a thick outer membrane. The phenotypic and genomic comparisons were conducted with one colistin-susceptible A. junii, which had the same antibiotic susceptibility profile except for colistin, and had the same KL25 capsule biosynthesis locus. The AJ6079 exhibited a slower growth rate, indicating that colistin-resistant A. junii possesses a higher fitness cost. The genome of AJ6079 had a G+C content of 38.7% and contained one 3,362,966 bp circular chromosome with no plasmid or mobile colistin resistance (mcr) gene. Comparative genomic analysis revealed that the AJ6079 contained several previously unreported point mutations in colistin-resistance-related genes involving amino acid substitutions in PmrB (N5K, G147C), LpxA (I107F, H131Y), and LpxD (F20I, K263R), which might be correlated with colistin resistance in A. junii. Further research is needed for verification as the genetic background was not exactly the same between the two isolates.
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spelling pubmed-97745292022-12-23 Emergence of Colistin-Resistant Acinetobacter junii in China Wang, Zhiren Fan, Xuanyang Wang, Shuyi Li, Shuguang Gao, Yue Wang, Hui Li, Henan Antibiotics (Basel) Article The increasing number of multidrug-resistant Gram-negative bacteria presents a serious threat to global health. However, colistin-resistant Acinetobacter junii has rarely been reported. We identified a colistin-resistant A. junii clinical isolate, AJ6079, in blood. The colony of AJ6079 presented a dry phenotype, and it was difficult to form a bacterial suspension, whilst transmission electron microscopy revealed that AJ6079 possessed a thick outer membrane. The phenotypic and genomic comparisons were conducted with one colistin-susceptible A. junii, which had the same antibiotic susceptibility profile except for colistin, and had the same KL25 capsule biosynthesis locus. The AJ6079 exhibited a slower growth rate, indicating that colistin-resistant A. junii possesses a higher fitness cost. The genome of AJ6079 had a G+C content of 38.7% and contained one 3,362,966 bp circular chromosome with no plasmid or mobile colistin resistance (mcr) gene. Comparative genomic analysis revealed that the AJ6079 contained several previously unreported point mutations in colistin-resistance-related genes involving amino acid substitutions in PmrB (N5K, G147C), LpxA (I107F, H131Y), and LpxD (F20I, K263R), which might be correlated with colistin resistance in A. junii. Further research is needed for verification as the genetic background was not exactly the same between the two isolates. MDPI 2022-11-24 /pmc/articles/PMC9774529/ /pubmed/36551350 http://dx.doi.org/10.3390/antibiotics11121693 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Wang, Zhiren
Fan, Xuanyang
Wang, Shuyi
Li, Shuguang
Gao, Yue
Wang, Hui
Li, Henan
Emergence of Colistin-Resistant Acinetobacter junii in China
title Emergence of Colistin-Resistant Acinetobacter junii in China
title_full Emergence of Colistin-Resistant Acinetobacter junii in China
title_fullStr Emergence of Colistin-Resistant Acinetobacter junii in China
title_full_unstemmed Emergence of Colistin-Resistant Acinetobacter junii in China
title_short Emergence of Colistin-Resistant Acinetobacter junii in China
title_sort emergence of colistin-resistant acinetobacter junii in china
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9774529/
https://www.ncbi.nlm.nih.gov/pubmed/36551350
http://dx.doi.org/10.3390/antibiotics11121693
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