Cargando…
GPX2 Gene Affects Feed Efficiency of Pigs by Inhibiting Fat Deposition and Promoting Muscle Development
SIMPLE SUMMARY: Glutathione peroxidase 2 (GPX2) gene expression differs between individuals with different GPX2 polymorphism. Individuals with high GPX2 expression had thinning 100 kg backfat (BF) thickness, no change on average daily gain (AG) and lower feed conversion ratio (FCR) and residual feed...
Autores principales: | , , , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2022
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9774625/ https://www.ncbi.nlm.nih.gov/pubmed/36552449 http://dx.doi.org/10.3390/ani12243528 |
_version_ | 1784855455220105216 |
---|---|
author | Pu, Lei Luo, Yunyan Wen, Zuochen Dai, Yuxin Zheng, Chunting Zhu, Xueli Qin, Lei Zhang, Chunguang Liang, Hong Zhang, Jianbin Guo, Liang Wang, Lixian |
author_facet | Pu, Lei Luo, Yunyan Wen, Zuochen Dai, Yuxin Zheng, Chunting Zhu, Xueli Qin, Lei Zhang, Chunguang Liang, Hong Zhang, Jianbin Guo, Liang Wang, Lixian |
author_sort | Pu, Lei |
collection | PubMed |
description | SIMPLE SUMMARY: Glutathione peroxidase 2 (GPX2) gene expression differs between individuals with different GPX2 polymorphism. Individuals with high GPX2 expression had thinning 100 kg backfat (BF) thickness, no change on average daily gain (AG) and lower feed conversion ratio (FCR) and residual feed intake (RFI) in Duroc pigs. The reason for this result could be that increasing GPX2 could inhibit the adipocyte proliferation and differentiation, promoting the lipid degradation. Meanwhile, GPX2 may promote muscle cells’ proliferation and myogenic differentiation. Overall, our fundings indicate that GPX2 can transfer more nutrients to less fat deposition direction, providing the material basis for animal weight gain. ABSTRACT: GPX2 has been recognized as a potential candidate gene for feed efficiency in pigs. This article aimed to elucidate polymorphism of GPX2 associated with feed efficiency and its related molecular mechanism. In this study, seven single nucleotide polymorphisms (SNP) of GPX2 were found among 383 Duroc pigs. In addition, seven SNPs and ALGA0043483 (PorcineSNP60 BeadChip data in 600 Duroc pigs), which are near the GPX2 gene, were identified in one haplotypes block. Furthermore, associated studies showed that the genotype of GPX2 has significant association with weaning weight and 100 kg BF in Duroc pigs. In addition, the AG had no effect when the backfat became thinner, and the FCR and RFI traits had a tendency to decrease in the G3 + TT combination genotype, accompanied by an increase of GPX2 expression in backfat and muscle tissues. At the cellular level, the adipocyte proliferation and ability of adipogenic differentiation were reduced, and the lipid degradation increased in 3T3-L1 when there was overexpression of GPX2. In contrast, overexpression of the GPX2 gene can promote the muscle cell proliferation and myogenic differentiation in C2C12 cells. In other words, GPX2 has the effect of reducing fat deposition and promoting muscle development, and it is a candidate gene for backfat and feed efficiency. |
format | Online Article Text |
id | pubmed-9774625 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-97746252022-12-23 GPX2 Gene Affects Feed Efficiency of Pigs by Inhibiting Fat Deposition and Promoting Muscle Development Pu, Lei Luo, Yunyan Wen, Zuochen Dai, Yuxin Zheng, Chunting Zhu, Xueli Qin, Lei Zhang, Chunguang Liang, Hong Zhang, Jianbin Guo, Liang Wang, Lixian Animals (Basel) Article SIMPLE SUMMARY: Glutathione peroxidase 2 (GPX2) gene expression differs between individuals with different GPX2 polymorphism. Individuals with high GPX2 expression had thinning 100 kg backfat (BF) thickness, no change on average daily gain (AG) and lower feed conversion ratio (FCR) and residual feed intake (RFI) in Duroc pigs. The reason for this result could be that increasing GPX2 could inhibit the adipocyte proliferation and differentiation, promoting the lipid degradation. Meanwhile, GPX2 may promote muscle cells’ proliferation and myogenic differentiation. Overall, our fundings indicate that GPX2 can transfer more nutrients to less fat deposition direction, providing the material basis for animal weight gain. ABSTRACT: GPX2 has been recognized as a potential candidate gene for feed efficiency in pigs. This article aimed to elucidate polymorphism of GPX2 associated with feed efficiency and its related molecular mechanism. In this study, seven single nucleotide polymorphisms (SNP) of GPX2 were found among 383 Duroc pigs. In addition, seven SNPs and ALGA0043483 (PorcineSNP60 BeadChip data in 600 Duroc pigs), which are near the GPX2 gene, were identified in one haplotypes block. Furthermore, associated studies showed that the genotype of GPX2 has significant association with weaning weight and 100 kg BF in Duroc pigs. In addition, the AG had no effect when the backfat became thinner, and the FCR and RFI traits had a tendency to decrease in the G3 + TT combination genotype, accompanied by an increase of GPX2 expression in backfat and muscle tissues. At the cellular level, the adipocyte proliferation and ability of adipogenic differentiation were reduced, and the lipid degradation increased in 3T3-L1 when there was overexpression of GPX2. In contrast, overexpression of the GPX2 gene can promote the muscle cell proliferation and myogenic differentiation in C2C12 cells. In other words, GPX2 has the effect of reducing fat deposition and promoting muscle development, and it is a candidate gene for backfat and feed efficiency. MDPI 2022-12-14 /pmc/articles/PMC9774625/ /pubmed/36552449 http://dx.doi.org/10.3390/ani12243528 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Pu, Lei Luo, Yunyan Wen, Zuochen Dai, Yuxin Zheng, Chunting Zhu, Xueli Qin, Lei Zhang, Chunguang Liang, Hong Zhang, Jianbin Guo, Liang Wang, Lixian GPX2 Gene Affects Feed Efficiency of Pigs by Inhibiting Fat Deposition and Promoting Muscle Development |
title | GPX2 Gene Affects Feed Efficiency of Pigs by Inhibiting Fat Deposition and Promoting Muscle Development |
title_full | GPX2 Gene Affects Feed Efficiency of Pigs by Inhibiting Fat Deposition and Promoting Muscle Development |
title_fullStr | GPX2 Gene Affects Feed Efficiency of Pigs by Inhibiting Fat Deposition and Promoting Muscle Development |
title_full_unstemmed | GPX2 Gene Affects Feed Efficiency of Pigs by Inhibiting Fat Deposition and Promoting Muscle Development |
title_short | GPX2 Gene Affects Feed Efficiency of Pigs by Inhibiting Fat Deposition and Promoting Muscle Development |
title_sort | gpx2 gene affects feed efficiency of pigs by inhibiting fat deposition and promoting muscle development |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9774625/ https://www.ncbi.nlm.nih.gov/pubmed/36552449 http://dx.doi.org/10.3390/ani12243528 |
work_keys_str_mv | AT pulei gpx2geneaffectsfeedefficiencyofpigsbyinhibitingfatdepositionandpromotingmuscledevelopment AT luoyunyan gpx2geneaffectsfeedefficiencyofpigsbyinhibitingfatdepositionandpromotingmuscledevelopment AT wenzuochen gpx2geneaffectsfeedefficiencyofpigsbyinhibitingfatdepositionandpromotingmuscledevelopment AT daiyuxin gpx2geneaffectsfeedefficiencyofpigsbyinhibitingfatdepositionandpromotingmuscledevelopment AT zhengchunting gpx2geneaffectsfeedefficiencyofpigsbyinhibitingfatdepositionandpromotingmuscledevelopment AT zhuxueli gpx2geneaffectsfeedefficiencyofpigsbyinhibitingfatdepositionandpromotingmuscledevelopment AT qinlei gpx2geneaffectsfeedefficiencyofpigsbyinhibitingfatdepositionandpromotingmuscledevelopment AT zhangchunguang gpx2geneaffectsfeedefficiencyofpigsbyinhibitingfatdepositionandpromotingmuscledevelopment AT lianghong gpx2geneaffectsfeedefficiencyofpigsbyinhibitingfatdepositionandpromotingmuscledevelopment AT zhangjianbin gpx2geneaffectsfeedefficiencyofpigsbyinhibitingfatdepositionandpromotingmuscledevelopment AT guoliang gpx2geneaffectsfeedefficiencyofpigsbyinhibitingfatdepositionandpromotingmuscledevelopment AT wanglixian gpx2geneaffectsfeedefficiencyofpigsbyinhibitingfatdepositionandpromotingmuscledevelopment |