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Anti-Biofilm Effect of Bacteriophages and Antibiotics against Uropathogenic Escherichia coli

Escherichia coli is a common cause of biofilm-associated urinary tract infections. Bacteria inside the biofilm are more resistant to antibiotics. Six E. coli strains isolated from patients with urinary tract infections were screened for biofilm-forming capability and antimicrobial susceptibility. Tw...

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Autores principales: Mukane, Laima, Racenis, Karlis, Rezevska, Dace, Petersons, Aivars, Kroica, Juta
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9774793/
https://www.ncbi.nlm.nih.gov/pubmed/36551363
http://dx.doi.org/10.3390/antibiotics11121706
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author Mukane, Laima
Racenis, Karlis
Rezevska, Dace
Petersons, Aivars
Kroica, Juta
author_facet Mukane, Laima
Racenis, Karlis
Rezevska, Dace
Petersons, Aivars
Kroica, Juta
author_sort Mukane, Laima
collection PubMed
description Escherichia coli is a common cause of biofilm-associated urinary tract infections. Bacteria inside the biofilm are more resistant to antibiotics. Six E. coli strains isolated from patients with urinary tract infections were screened for biofilm-forming capability and antimicrobial susceptibility. Two of the most significant biofilm-producing strains were selected for minimal inhibitory concentration and minimal biofilm eradication concentration in vitro testing using amoxicillin–clavulanic acid, ciprofloxacin, and three commercial bacteriophage cocktails (Pyobacteriophag, Ses, and Intesti). In case of a low phage effect, an adaptation procedure was performed. Although the biofilms formed by strain 021UR were resistant to amoxicillin–clavulanic acid and ciprofloxacin, the three phage cocktails were able to reduce biofilm formation. In contrast, phages did not affect the 01206UR strain against planktonic and biofilm-forming cells. After Pyobacteriophag adaptation, the effect improved, and, regardless of the concentration, the adapted phage cocktail could destroy both planktonic cells and the biofilm of strain 01206UR. Bacteriophages capable of killing bacteria in biofilms can be used as an alternative to antibiotics. However, each case should be considered individually due to the lack of clinical trials for phage therapy. Antimicrobial and phage susceptibility should be determined in biofilm models before treatment to achieve the desired anti-biofilm effect.
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spelling pubmed-97747932022-12-23 Anti-Biofilm Effect of Bacteriophages and Antibiotics against Uropathogenic Escherichia coli Mukane, Laima Racenis, Karlis Rezevska, Dace Petersons, Aivars Kroica, Juta Antibiotics (Basel) Article Escherichia coli is a common cause of biofilm-associated urinary tract infections. Bacteria inside the biofilm are more resistant to antibiotics. Six E. coli strains isolated from patients with urinary tract infections were screened for biofilm-forming capability and antimicrobial susceptibility. Two of the most significant biofilm-producing strains were selected for minimal inhibitory concentration and minimal biofilm eradication concentration in vitro testing using amoxicillin–clavulanic acid, ciprofloxacin, and three commercial bacteriophage cocktails (Pyobacteriophag, Ses, and Intesti). In case of a low phage effect, an adaptation procedure was performed. Although the biofilms formed by strain 021UR were resistant to amoxicillin–clavulanic acid and ciprofloxacin, the three phage cocktails were able to reduce biofilm formation. In contrast, phages did not affect the 01206UR strain against planktonic and biofilm-forming cells. After Pyobacteriophag adaptation, the effect improved, and, regardless of the concentration, the adapted phage cocktail could destroy both planktonic cells and the biofilm of strain 01206UR. Bacteriophages capable of killing bacteria in biofilms can be used as an alternative to antibiotics. However, each case should be considered individually due to the lack of clinical trials for phage therapy. Antimicrobial and phage susceptibility should be determined in biofilm models before treatment to achieve the desired anti-biofilm effect. MDPI 2022-11-26 /pmc/articles/PMC9774793/ /pubmed/36551363 http://dx.doi.org/10.3390/antibiotics11121706 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Mukane, Laima
Racenis, Karlis
Rezevska, Dace
Petersons, Aivars
Kroica, Juta
Anti-Biofilm Effect of Bacteriophages and Antibiotics against Uropathogenic Escherichia coli
title Anti-Biofilm Effect of Bacteriophages and Antibiotics against Uropathogenic Escherichia coli
title_full Anti-Biofilm Effect of Bacteriophages and Antibiotics against Uropathogenic Escherichia coli
title_fullStr Anti-Biofilm Effect of Bacteriophages and Antibiotics against Uropathogenic Escherichia coli
title_full_unstemmed Anti-Biofilm Effect of Bacteriophages and Antibiotics against Uropathogenic Escherichia coli
title_short Anti-Biofilm Effect of Bacteriophages and Antibiotics against Uropathogenic Escherichia coli
title_sort anti-biofilm effect of bacteriophages and antibiotics against uropathogenic escherichia coli
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9774793/
https://www.ncbi.nlm.nih.gov/pubmed/36551363
http://dx.doi.org/10.3390/antibiotics11121706
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