Mycorrhizal Colonization Modulates the Essential Oil Profile and Enzymatic and Non-Enzymatic Antioxidants to Mitigate the Adverse Effects of Water Deficit in Salvia subg. Perovskia

SIMPLE SUMMARY: We explored the modulating effect of arbuscular mycorrhizal fungi symbiosis on the water deficit stress symptoms in two medicinal and aromatic plants—Salvia abrotanoides and S. yangii. The essential oil content and composition, along with the enzymatic and non-enzymatic antioxidants, were studied to discover that the mycorrhizal fungi inoculation alleviated the deleterious impacts of water stress and influenced significantly the composition and content of essential oils. These effects may be explained by improved phosphorus uptake, chlorophyll biosynthesis, relative water content, and enzymatic and non-enzymatic antioxidant activities. Taken together, these findings highlighted the role of symbiosis with mycorrhizal fungi in increasing the tolerance to water deficit stress in medicinal and aromatic plants. ABSTRACT: Among traditional Iranian herbs, Perovskia species (a subgenus of Salvia), while being valued ornamentals, are also studied for numerous potential pharmacological and therapeutic aspects. The current study was conducted to assess the effectiveness of two species of arbuscular mycorrhizal fungi (AMF), Funneliformis mosseae and Rhizophagus intraradices, separately and in combination, in terms of the essential oil content and compositions along with the enzymatic and non-enzymatic antioxidants in Salvia abrotanoides and S. yangii in response to three levels of irrigation, including 100% FC as well-watered, 75% FC (moderate irrigation deficit), and 50% FC (severe irrigation deficit). In both species, essential oil content, enzyme antioxidant activities, total phenolics, and flavonoids were increased significantly with the severity of stress; this increase was more pronounced in mycorrhizal inoculated herbs. Furthermore, leaf phosphorus concentration, relative water content, chlorophylls a and b, and total carotenoids decreased in parallel with reducing soil moisture; albeit, AMF inoculation improved the stress symptoms under increasing severity of water restriction compared with their control conditions. In addition, the percentage of root colonization was positively correlated with the relative water content (RWC) and leaf phosphorus concentration. Taking into account the essential oil groups, AMF colonization elevated some essential oil components, such as oxygenated monoterpenes, 1,8-cineol, camphor, and borneol, whereas the main sesquiterpenes, including E-β-caryophyllene and α-humulene, remarkably decreased. Taken together, these findings highlighted the role of symbiosis with AMFs in increasing the tolerance of water deficit stress in S. abrotanoides and S. yangii and improving their essential oil composition.