Cargando…

Analysis of Polyphenolic Compounds in Water-Based Extracts of Vicia faba L.: A Potential Innovative Source of Nutraceutical Ingredients

The water-based extract of broad bean hulls contains several bioactive molecules, including polyphenols well-known to exert antioxidant activity, which could justify its use in nutraceutical formulations. Hence, the current investigation aimed to establish the polyphenolic profile of water-based ext...

Descripción completa

Detalles Bibliográficos
Autores principales: Castaldo, Luigi, Izzo, Luana, Lombardi, Sonia, Gaspari, Anna, De Pascale, Stefania, Grosso, Michela, Ritieni, Alberto
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9774889/
https://www.ncbi.nlm.nih.gov/pubmed/36552661
http://dx.doi.org/10.3390/antiox11122453
_version_ 1784855509779611648
author Castaldo, Luigi
Izzo, Luana
Lombardi, Sonia
Gaspari, Anna
De Pascale, Stefania
Grosso, Michela
Ritieni, Alberto
author_facet Castaldo, Luigi
Izzo, Luana
Lombardi, Sonia
Gaspari, Anna
De Pascale, Stefania
Grosso, Michela
Ritieni, Alberto
author_sort Castaldo, Luigi
collection PubMed
description The water-based extract of broad bean hulls contains several bioactive molecules, including polyphenols well-known to exert antioxidant activity, which could justify its use in nutraceutical formulations. Hence, the current investigation aimed to establish the polyphenolic profile of water-based extracts from broad bean hulls through UHPLC–Q-Orbitrap HRMS analysis. The findings highlighted that p-coumaric acid, chlorogenic acid, and epicatechin were the most common compounds found in the tested extracts, being quantified at a mean concentration of 42.1, 32.6, and 31.2 mg/100 g, respectively. Moreover, broad bean hull extracts were encapsulated into a nutraceutical formulation, after which the antioxidant properties and the bioaccessibility of phenolic compounds during the simulated gastrointestinal (GI) process were investigated and compared with the digested non-encapsulated extract. The data highlighted that following the GI process, the capsules were able to preserve active compounds from the adverse effects of digestion, resulting in a greater antioxidant capacity and polyphenol bioaccessibility in the duodenal and colonic phases, compared with the non-encapsulated extract. Our results showed that the water extract from broad bean hulls may be considered a valuable source of natural polyphenolic compounds; in addition, the use of a gastric-resistant capsule could be a suitable alternative to transport these bioactive compounds to the target tissues.
format Online
Article
Text
id pubmed-9774889
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-97748892022-12-23 Analysis of Polyphenolic Compounds in Water-Based Extracts of Vicia faba L.: A Potential Innovative Source of Nutraceutical Ingredients Castaldo, Luigi Izzo, Luana Lombardi, Sonia Gaspari, Anna De Pascale, Stefania Grosso, Michela Ritieni, Alberto Antioxidants (Basel) Article The water-based extract of broad bean hulls contains several bioactive molecules, including polyphenols well-known to exert antioxidant activity, which could justify its use in nutraceutical formulations. Hence, the current investigation aimed to establish the polyphenolic profile of water-based extracts from broad bean hulls through UHPLC–Q-Orbitrap HRMS analysis. The findings highlighted that p-coumaric acid, chlorogenic acid, and epicatechin were the most common compounds found in the tested extracts, being quantified at a mean concentration of 42.1, 32.6, and 31.2 mg/100 g, respectively. Moreover, broad bean hull extracts were encapsulated into a nutraceutical formulation, after which the antioxidant properties and the bioaccessibility of phenolic compounds during the simulated gastrointestinal (GI) process were investigated and compared with the digested non-encapsulated extract. The data highlighted that following the GI process, the capsules were able to preserve active compounds from the adverse effects of digestion, resulting in a greater antioxidant capacity and polyphenol bioaccessibility in the duodenal and colonic phases, compared with the non-encapsulated extract. Our results showed that the water extract from broad bean hulls may be considered a valuable source of natural polyphenolic compounds; in addition, the use of a gastric-resistant capsule could be a suitable alternative to transport these bioactive compounds to the target tissues. MDPI 2022-12-12 /pmc/articles/PMC9774889/ /pubmed/36552661 http://dx.doi.org/10.3390/antiox11122453 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Castaldo, Luigi
Izzo, Luana
Lombardi, Sonia
Gaspari, Anna
De Pascale, Stefania
Grosso, Michela
Ritieni, Alberto
Analysis of Polyphenolic Compounds in Water-Based Extracts of Vicia faba L.: A Potential Innovative Source of Nutraceutical Ingredients
title Analysis of Polyphenolic Compounds in Water-Based Extracts of Vicia faba L.: A Potential Innovative Source of Nutraceutical Ingredients
title_full Analysis of Polyphenolic Compounds in Water-Based Extracts of Vicia faba L.: A Potential Innovative Source of Nutraceutical Ingredients
title_fullStr Analysis of Polyphenolic Compounds in Water-Based Extracts of Vicia faba L.: A Potential Innovative Source of Nutraceutical Ingredients
title_full_unstemmed Analysis of Polyphenolic Compounds in Water-Based Extracts of Vicia faba L.: A Potential Innovative Source of Nutraceutical Ingredients
title_short Analysis of Polyphenolic Compounds in Water-Based Extracts of Vicia faba L.: A Potential Innovative Source of Nutraceutical Ingredients
title_sort analysis of polyphenolic compounds in water-based extracts of vicia faba l.: a potential innovative source of nutraceutical ingredients
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9774889/
https://www.ncbi.nlm.nih.gov/pubmed/36552661
http://dx.doi.org/10.3390/antiox11122453
work_keys_str_mv AT castaldoluigi analysisofpolyphenoliccompoundsinwaterbasedextractsofviciafabalapotentialinnovativesourceofnutraceuticalingredients
AT izzoluana analysisofpolyphenoliccompoundsinwaterbasedextractsofviciafabalapotentialinnovativesourceofnutraceuticalingredients
AT lombardisonia analysisofpolyphenoliccompoundsinwaterbasedextractsofviciafabalapotentialinnovativesourceofnutraceuticalingredients
AT gasparianna analysisofpolyphenoliccompoundsinwaterbasedextractsofviciafabalapotentialinnovativesourceofnutraceuticalingredients
AT depascalestefania analysisofpolyphenoliccompoundsinwaterbasedextractsofviciafabalapotentialinnovativesourceofnutraceuticalingredients
AT grossomichela analysisofpolyphenoliccompoundsinwaterbasedextractsofviciafabalapotentialinnovativesourceofnutraceuticalingredients
AT ritienialberto analysisofpolyphenoliccompoundsinwaterbasedextractsofviciafabalapotentialinnovativesourceofnutraceuticalingredients