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Interorgan Metabolism of Ganglioside Is Altered in Type 2 Diabetes

GM3 is implicated in cell signaling, inflammation and insulin resistance. The intestinal mucosa metabolizes ganglioside and provides gangliosides for uptake by peripheral tissues. Gangliosides downregulate acute and chronic inflammatory signals. It is likely that transport of intestinal derived gang...

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Autores principales: Rivas Serna, Irma Magaly, Beveridge, Michelle, Wilke, Michaelann, Ryan, Edmond A., Clandinin, Michael Thomas, Mazurak, Vera Christine
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9775016/
https://www.ncbi.nlm.nih.gov/pubmed/36551897
http://dx.doi.org/10.3390/biomedicines10123141
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author Rivas Serna, Irma Magaly
Beveridge, Michelle
Wilke, Michaelann
Ryan, Edmond A.
Clandinin, Michael Thomas
Mazurak, Vera Christine
author_facet Rivas Serna, Irma Magaly
Beveridge, Michelle
Wilke, Michaelann
Ryan, Edmond A.
Clandinin, Michael Thomas
Mazurak, Vera Christine
author_sort Rivas Serna, Irma Magaly
collection PubMed
description GM3 is implicated in cell signaling, inflammation and insulin resistance. The intestinal mucosa metabolizes ganglioside and provides gangliosides for uptake by peripheral tissues. Gangliosides downregulate acute and chronic inflammatory signals. It is likely that transport of intestinal derived gangliosides to other tissues impact the same signals characteristic of inflammatory change in other chronic conditions such as Type 2 Diabetes (T2DM). The postprandial ceramide composition of GM3 and other gangliosides in plasma and chylomicrons has not been examined in T2DM. The present study assessed if diet or T2DM alters ganglioside components in plasma and chylomicrons secreted from the intestinal mucosa after a meal. GD1, GD3, and GM3 content of chylomicrons and plasma was determined by LC/triple quad MS in non-diabetic (control) and T2DM individuals in the fasting and postprandial state after 2 days of consuming a low or high fat diet in a randomized blinded crossover design. Diet fat level did not alter baseline plasma or chylomicron ganglioside levels. Four hours after the test meal, plasma monounsaturated GD3 was 75% higher, plasma saturated GD3 was 140% higher and plasma polyunsaturated GM3 30% lower in diabetic subjects compared to control subjects. At 4 h, chylomicron GD1 was 50% lower in T2DM compared to controls. The proportion of d34:1 in GD3 was more abundant and d36:1 in GD1 less abundant in T2DM compared to control subjects at 4 h. The present study indicates that T2DM alters ceramide composition of ganglioside available for uptake by peripheral tissues.
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spelling pubmed-97750162022-12-23 Interorgan Metabolism of Ganglioside Is Altered in Type 2 Diabetes Rivas Serna, Irma Magaly Beveridge, Michelle Wilke, Michaelann Ryan, Edmond A. Clandinin, Michael Thomas Mazurak, Vera Christine Biomedicines Article GM3 is implicated in cell signaling, inflammation and insulin resistance. The intestinal mucosa metabolizes ganglioside and provides gangliosides for uptake by peripheral tissues. Gangliosides downregulate acute and chronic inflammatory signals. It is likely that transport of intestinal derived gangliosides to other tissues impact the same signals characteristic of inflammatory change in other chronic conditions such as Type 2 Diabetes (T2DM). The postprandial ceramide composition of GM3 and other gangliosides in plasma and chylomicrons has not been examined in T2DM. The present study assessed if diet or T2DM alters ganglioside components in plasma and chylomicrons secreted from the intestinal mucosa after a meal. GD1, GD3, and GM3 content of chylomicrons and plasma was determined by LC/triple quad MS in non-diabetic (control) and T2DM individuals in the fasting and postprandial state after 2 days of consuming a low or high fat diet in a randomized blinded crossover design. Diet fat level did not alter baseline plasma or chylomicron ganglioside levels. Four hours after the test meal, plasma monounsaturated GD3 was 75% higher, plasma saturated GD3 was 140% higher and plasma polyunsaturated GM3 30% lower in diabetic subjects compared to control subjects. At 4 h, chylomicron GD1 was 50% lower in T2DM compared to controls. The proportion of d34:1 in GD3 was more abundant and d36:1 in GD1 less abundant in T2DM compared to control subjects at 4 h. The present study indicates that T2DM alters ceramide composition of ganglioside available for uptake by peripheral tissues. MDPI 2022-12-06 /pmc/articles/PMC9775016/ /pubmed/36551897 http://dx.doi.org/10.3390/biomedicines10123141 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Rivas Serna, Irma Magaly
Beveridge, Michelle
Wilke, Michaelann
Ryan, Edmond A.
Clandinin, Michael Thomas
Mazurak, Vera Christine
Interorgan Metabolism of Ganglioside Is Altered in Type 2 Diabetes
title Interorgan Metabolism of Ganglioside Is Altered in Type 2 Diabetes
title_full Interorgan Metabolism of Ganglioside Is Altered in Type 2 Diabetes
title_fullStr Interorgan Metabolism of Ganglioside Is Altered in Type 2 Diabetes
title_full_unstemmed Interorgan Metabolism of Ganglioside Is Altered in Type 2 Diabetes
title_short Interorgan Metabolism of Ganglioside Is Altered in Type 2 Diabetes
title_sort interorgan metabolism of ganglioside is altered in type 2 diabetes
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9775016/
https://www.ncbi.nlm.nih.gov/pubmed/36551897
http://dx.doi.org/10.3390/biomedicines10123141
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